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Screening and preliminary evaluation of oceanic microalgae for bioenergy production

WANG Nengfei 1,2,SUN Yunfei 2,3,WANG Shuchun2,YI Dan2,XU Bin2,WANG Fengqin3,ZANG Jiaye2   

  1. 1College of Life Sciences,Ocean University of China,Qingdao 266100,Shandong,China;2First Institute of Oceanography,SOA,Qingdao 266061,Shandong,China;3College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,Henan,China
  • Online:2013-10-05 Published:2013-10-05

海洋能源微藻的筛选及初步评价

王能飞1,2,孙云飞2,3,王树春2,衣 丹2,许 宾2,王风芹3,臧家业2   

  1. 1中国海洋大学海洋生命学院,山东 青岛 266100;2国家海洋局第一海洋研究所,山东 青岛 266061; 3河南农业大学生命科学学院,河南 郑州 450002

Abstract: The water samples were collected from south part of the Yellow Sea. Two hundred and forty microalgae were isolated and purified from the above samples using a capillary method. Among them,eight fast-growing algae strains were cultured in large scale. The lyophilized powder of the above algae cultures were prepared for the analysis of starch,cellulose,soluble sugar and total fat. One algae strain named Navicula sp. NB-2 yielded high dry biomass and total fat (413.3 mg/g dry biomass),and therefore was selected as the initial strain for biodiesel production in future. Another stain named Navicula sp.NB-3 had fast-growing rate,high cellulose content (92.6 mg/g dry biomass) and high soluble sugar amount (223 mg/g dry biomass). Moreover,Navicula sp.NB-3 yielded high bioethanol amount (77.5 mg/g dry biomass) and had potential for bioethanol production.

Key words: energy microalgae, cellulose, soluble polysaccharides, bioethanol

摘要: 从黄海、南海部分海域采集水样,分离纯化了240株微藻,对其进行了分类与鉴定,从其中挑选8株生长较快的藻种进行扩大培养。经离心,冷冻干燥后得到藻粉,进而对藻种的淀粉、纤维素、总脂及可溶性糖含量进行了测量,以期筛选到目标能源微藻藻种。藻株Navicula sp.TW-2的油脂含量达到41.3%,生长速度快,生物量也较大,可以作为制备生物柴油的出发藻种。藻株Nannochloropsis sp.NB-3的纤维素含量达到9.26%,可溶性糖含量达到22.3%,生物量大,经过预处理发酵后生物乙醇产率可达7.75%,是制备生物燃料乙醇的优良藻株。

关键词: 能源微藻, 纤维素, 可溶性多糖, 生物乙醇

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