Abstract: Cellobiose was often found in cellulose hydrolysates. In this study，the ability to use cellobiose to produce succinic acid by A.succinogenes NJ113 and using cellobiose prepared from sugarcane bagasse cellulose hydrolysates as a carbon source to produce succinic acid were investigated. A final succinic acid concentration of 23.51 g/L with a yield of 67.17% was achieved from an initial cellobiose concentration of 35 g/L via batch fermentation. In batch fermentation with 18 g/L of cellobiose and 17 g/L of other sugars from sugarcane bagasse cellulose hydrolysates，a succinic acid concentration of 20.00 g/L was obtained，with a yield of 64.73%. This study suggested that A. succinogenes had a strong ability to convert cellobiose into succinic acid and cellobiose from cellulose hydrolysate could be a potential carbon source for economical and efficient succinic acid production by A. succinogenes.

Key words: succinic acid, fermentation, cellobiose, cellulose hydrolysate, Actinobacillus succinogenes NJ113

摘要： 纤维素水解液中通常含有纤维二糖。本文考察了Actinobacillus succinogenes NJ113利用纤维二糖厌氧发酵生产丁二酸的能力，并利用蔗渣纤维素制备纤维二糖作为碳源用于厌氧发酵生产丁二酸。3 L发酵罐厌氧发酵结果显示：以35 g/L纤维二糖作为碳源发酵制备丁二酸，其产量为23.51 g/L，产率达到67.17%；用含有18 g/L纤维二糖和17 g/L其它糖类的蔗渣纤维素水解液作为碳源发酵制备丁二酸，丁二酸的产量和产率分别为20.00 g/L和64.73%。因此，Actinobacillus succinogenes NJ113具有较强的利用纤维二糖生产丁二酸的能力，而且利用废弃的纤维素制备纤维二糖作为碳源高效、经济地发酵制备丁二酸具有可行性。

关键词: 丁二酸, 发酵, 纤维二糖, 纤维素水解液, Actinobacillus succinogenes NJ113