A novel anaerobic hydrolysis acidification-partial denitrification anaerobic ammonia oxidation process for advanced nitrogen removal from simulated domestic and nitrate-containing wastewater

doi:10.16085/j.issn.1000-6613.2021-1775

Abstract

Abstract:

A new anaerobic hydrolytic acidification (AnHA)-partial denitrification anaerobic ammonia oxidation (PD/A) process was constructed to realize the simultaneous and efficient treatment of simulated domestic sewage with low carbon nitrogen ratio and low concentration nitrate wastewater. By controlling the influent NO₃^--N/NH₄⁺-N=1.2 and COD/TN=2.36, and adjusting the subsection influent ratio of domestic sewage to 3∶7 and AnHA reactor HRT=3.2h, AnHA-PD/A system had achieved 94.78% TN removal, and the corresponding effluent TN concentration was only 5.47mg/L, which was far lower than the level A discharge standard of urban sewage treatment plants in China. During the stable operation, PD-Anammox process was the most important nitrogen removal process in the AnHA-PD/A system, whose contribution rate to TN removal was as high as 95.87%. The results of gas chromatography showed that acetic acid, as the main organic component of AnHA effluent (43.65%), that is, high-quality carbon source supply, greatly promoted the supply process of NO₂^--N in the PD/A system. Microbial high-throughput sequencing showed that Commonas and Omatilinea, as the hydrolysis and acidification bacteria with the highest relative abundance in the AnHA system, played an important role in the degradation and acid production of macromolecular organics, with corresponding abundances of 2.97% and 3.74% respectively, and the main dominant functional bacteria in the PD/A system were CandidatusBrocadia and Thauera, with corresponding abundances of 2.93% and 7.37% respectively.

Key words: anaerobic hydrolysis acidification (AnHA), partial denitrification anaerobic ammonia oxidation (PD/A), segmented water inflow, NO₃^--N/NH₄⁺-N

摘要：

构建新型厌氧水解酸化（AnHA）-短程反硝化厌氧氨氧化（PD/A）工艺，实现了低碳氮比模拟生活污水和低浓度硝酸盐模拟废水的同步和高效处理。通过控制进水NO₃^--N/NH₄⁺-N=1.2、COD/TN=2.36，调控生活污水分段进水比为3∶7，AnHA反应器HRT=3.2h，AnHA-PD/A系统实现了94.78% TN去除，相应出水TN浓度仅为5.47mg/L，远低于我国城镇污水处理厂一级A排放标准。稳定运行期间，PD-Anammox过程作为AnHA-PD/A系统内最为主要的氮素去除过程，其对系统TN去除贡献率高达95.87%。气相色谱结果表明，乙酸作为AnHA出水主要有机成分（43.65%），即优质碳源供给极大地促进了PD/A系统内NO₂^--N供给过程。微生物高通量测序表明，Commamonas和Omatilinea作为AnHA系统内相对丰度最高的水解和酸化菌属，在大分子有机物的降解产酸过程中发挥重要作用，相应丰度分别为2.97%和3.74%；PD/A系统内主要优势功能菌属为Candidatus Brocadia和Thauera，相应丰度分别为2.93%和7.37%。

关键词: 厌氧水解酸化, 短程反硝化厌氧氨氧化, 分段进水, NO₃^--N/NH₄⁺-N

CLC Number:

X703

WANG Chaochao, WU Yiling, CHEN Jiaqiao, CAI Tianning, LIU Wenru, LI Xiang, WU Peng. A novel anaerobic hydrolysis acidification-partial denitrification anaerobic ammonia oxidation process for advanced nitrogen removal from simulated domestic and nitrate-containing wastewater[J]. Chemical Industry and Engineering Progress, 2022, 41(7): 3890-3899.

王超超, 吴翼伶, 陈嘉巧, 蔡天宁, 刘文如, 李祥, 吴鹏. 新型厌氧水解酸化-短程反硝化厌氧氨氧化工艺同步处理生活污水和含硝酸盐模拟废水[J]. 化工进展, 2022, 41(7): 3890-3899.

Figures/Tables 7

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运行特征	NH₄⁺-N/mg·L^-1	COD/mg·L^-1	NO₃^--N/mg·L^-1	Q₁/Q₂	生活污水分段进水比（a∶b）	AnHA 反应器HRT/h	PD/A 反应器HRT/h
阶段Ⅰ-1（1~30天）	50	260	50	1.0	10∶0	0	6
阶段Ⅰ-2（31~62天）	50	260	60	1.2	10∶0	0	6
阶段Ⅱ-1（63~90天）	50	260	60	1.2	5∶5	4.5	6
阶段Ⅱ-2（91~132天）	50	260	60	1.2	3∶7	3.2	6
阶段Ⅱ-3（133~170天）	50	260	60	1.2	1∶9	2.5	6

运行特征	NH₄⁺-N/mg·L^-1	COD/mg·L^-1	NO₃^--N/mg·L^-1	Q₁/Q₂	生活污水分段进水比（a∶b）	AnHA 反应器HRT/h	PD/A 反应器HRT/h
阶段Ⅰ-1（1~30天）	50	260	50	1.0	10∶0	0	6
阶段Ⅰ-2（31~62天）	50	260	60	1.2	10∶0	0	6
阶段Ⅱ-1（63~90天）	50	260	60	1.2	5∶5	4.5	6
阶段Ⅱ-2（91~132天）	50	260	60	1.2	3∶7	3.2	6
阶段Ⅱ-3（133~170天）	50	260	60	1.2	1∶9	2.5	6

模拟生活污水				含硝酸盐模拟废水
COD/mg·L^-1	NH₄⁺-N/mg·L^-1	NO₂^--N/mg·L^-1		NO₃^--N/mg·L^-1
260	50	0~0.3		50~60

模拟生活污水				含硝酸盐模拟废水
COD/mg·L^-1	NH₄⁺-N/mg·L^-1	NO₂^--N/mg·L^-1		NO₃^--N/mg·L^-1
260	50	0~0.3		50~60

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