Chemical Industry and Engineering Progress ›› 2023, Vol. 42 ›› Issue (6): 3272-3280.DOI: 10.16085/j.issn.1000-6613.2022-1461

• Resources and environmental engineering • Previous Articles     Next Articles

Effects of hydroxylamine on the activity and kinetic parameters of nitrifying bacteria

ZENG Tianxu1,2,3(), ZHANG Yongxian4, YAN Yuan1,2,3, LIU Hong1,2,3, MA Jiao1,2,3, DANG Hongzhong1,2,3, WU Xinbo1,2,3, LI Weiwei1,2,3,4, CHEN Yongzhi1,2,3()   

  1. 1.Key Laboratory of Yellow River Water Environment in Gansu Province, Lanzhou Jiaotong University, Lanzhou 730070, Gansu, China
    2.School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, Gansu, China
    3.Technical Center of Sewage Treatment Industry in Gansu Province, Lanzhou 730070, Gansu, China
    4.Gansu Research Institute of Light Industry Company, Limited, Lanzhou 730000, Gansu, China
  • Received:2022-08-04 Revised:2022-09-28 Online:2023-06-29 Published:2023-06-25
  • Contact: CHEN Yongzhi

羟胺对硝化菌活性及其动力学参数的影响

曾天续1,2,3(), 张永显4, 严渊1,2,3, 刘宏1,2,3, 马娇1,2,3, 党鸿钟1,2,3, 吴新波1,2,3, 李维维1,2,3,4, 陈永志1,2,3()   

  1. 1.兰州交通大学甘肃省黄河水环境重点实验室,甘肃 兰州 730070
    2.兰州交通大学环境与市政工程学院,甘肃 兰州 730070
    3.甘肃省污水处理行业技术中心,甘肃 兰州 730070
    4.甘肃省轻工研究院有限责任公司,甘肃 兰州 730000
  • 通讯作者: 陈永志
  • 作者简介:曾天续(1995—),女,硕士研究生,研究方向为污(废)水处理理论与技术。E-mail:1961434363@qq.com
  • 基金资助:
    甘肃省科技计划(20JR2RA002);甘肃省黄河水环境重点实验室开放基金(21YRWEK006);甘肃省高等学校产业支撑计划(2020C-38);兰州交通大学天佑创新团队(TY2020XX);兰州市科技计划(2021-1-180);甘肃省青年科技基金计划(20JR5RA075)

Abstract:

The effects of hydroxylamine (NH2OH) on the activity and kinetic parameters of ammonia oxidizing bacteria (AOB) and the recovery of AOB activity after inhibition were investigated using SBR to treat synthetic domestic sewage. Results of batch test showed that when the concentration of NH2OH was 4.5mg/L, the activity of AOB showed the highest level, the activity of nitrite oxidizing bacteria (NOB) decreased, and the oxidation rate of NO2--N (QmaxNOB) decreased to 8.00mg/(L·h). The half-saturation constant of NO2--N (KNO2NOB) increased to 7.77mg/L, indicating that the inhibition type of NH2OH on NOB was mixed inhibition. In the long-term experiments, R1 and R2 were operated in anoxic/aerobic, and R3 was operated in aerobic/anoxic mode four times alternately per day. NH2OH was added into R2 and R3 reactors and not in R1. The results showed that, there was no nitrite accumulation in R1. When the concentration of NH2OH was 4.5mg/L in R2, the activity of AOB was (6.04±0.4)mgN/(gMLVSS·h) on the 6th day. When the concentration of NH2OH was 10mg/L, the activities of AOB and NOB decreased to (0.16±0.1)mgN/(gMLVSS·h) and (0.15±0.1)mgN/(gMLVSS·h), respectively. The activities of AOB recovered on the 5th day under high strength aeration without adding NH2OH. When the concentration of NH2OH was 4.5mg/L in R3, the ratio of NO2--N/NH4+-N in effluent was 1.2 on the 7th day. High-throughput sequencing of 16S rRNA showed that the relative abundance in R2 and R3 of AOB and NOB were 6.6%, 1.3%, and 3.0%, 1.9% when the concentration of NH2OH was 4.5mg/L. Ellin6067 and Nitrosomonas belonged to AOB, and Unidentified Nitro spiraceae belonged to NOB.

Key words: hydroxylamine (NH2OH), Ammonia oxidizing bacteria (AOB), nitrite oxidizing bacteria (NOB), partial nitrification, AOB activity recovery, kinetic parameter

摘要:

采用序批式间歇反应器(SBR)处理模拟生活污水,研究羟胺(NH2OH)对硝化菌活性、动力学参数及其对氨氧化菌(AOB)抑制后,AOB活性恢复的影响。批次试验结果表明,当NH2OH浓度为4.5mg/L时,AOB活性最大,亚硝酸盐氧化菌(NOB)活性减小,并且NO2--N氧化速率(QmaxNOB)减小至8.00mg/(L·h),NO2--N半饱和常数(KNO2NOB)增大至7.77mg/L,表明NH2OH对NOB的抑制类型为混合抑制。长期试验R1、R2以缺氧/好氧模式运行,R3以好氧/缺氧交替4次运行,R1不投加NH2OH,R2、R3投加NH2OH。结果表明,R1中全程无亚硝积累。在R2中投加4.5mg/L NH2OH,AOB活性为(6.04±0.4)mgN/(gMLVSS·h)。当NH2OH浓度为10mg/L时,AOB和NOB的活性分别下降至(0.16±0.1)mgN/(gMLVSS·h)和(0.15±0.1)mgN/(gMLVSS·h),之后在高曝气且不投加NH2OH的条件下,AOB活性在第5天恢复。在R3中投加4.5mg/L NH2OH,第7天出水NO2--N/NH4+-N为1.2。16S rRNA高通量测序表明,添加4.5mg/L NH2OH后,R2和R3中AOB、NOB的相对丰度分别为6.6%、1.3%和3.0%、1.9%,AOB菌属为Ellin6067和Nitrosomonas,NOB菌属为Unidentified Nitrospiraceae

关键词: 羟胺, 氨氧化菌, 亚硝酸盐氧化菌, 短程硝化, AOB活性恢复, 动力学参数

CLC Number: 

京ICP备12046843号-2;京公网安备 11010102001994号
Copyright © Chemical Industry and Engineering Progress, All Rights Reserved.
E-mail: hgjz@cip.com.cn
Powered by Beijing Magtech Co. Ltd