Rapid start-up and microbial community characteristics of a micro-aerobic methane-producing EGSBBR system

doi:10.16085/j.issn.1000-6613.2020-0295

Abstract

Abstract:

To achieve rapid start-up of methanogenesis of high-concentration organic wastewater, an EGSBBR system was used to treat influent COD concentration of 2000mg/L. Methanation start-up was realized after 34 days operation through gradually shortening hydraulic retention time and increasing ascending flow velocity of the reaction zone, and the corresponding COD removal efficiency increased from 67.5% to 94.2% with the influent organic loading rate up to 1.0kgCOD/(m³·d) and the maximum methane yield of the system being 582mL/d. The methane production was correlated with the change of effluent pH and VFA concentration, and the concentrations of polysaccharide and protein in sludge EPS increased significantly. High-throughput sequencing revealed that Methanothrix was the main abundant genus, indicating that methane was mainly formed by the decarboxylation of acetic acid under microaerobic conditions. This study offers a novel reference process for rapidly achieving methanogenesis of high-strength organic wastewater treatment.

Key words: expanded granular sludge blanket biofilm reactor (EGSBBR), start-up, methanogenesis, EPS, microbial community

摘要：

为实现高有机废水产甲烷快速启动，本文采用微氧膨胀颗粒污泥床生物膜反应器（EGSBBR）处理2000mg/L化学需氧量（COD）有机废水，通过逐步缩短水力停留时间和增大反应区上升流速的方式，经过34天运行系统产甲烷成功启动，COD去除率从67.5%升高至94.2%，有机负荷达1.0kgCOD/(m³·d)，此时系统最大甲烷产量为582mL/d。产气量与出水pH和出水VFA浓度变化具有相关性，且污泥胞外聚合物（EPS）中的多糖、蛋白质浓度显著增加。高通量测序结果证实Methanothrix为系统产甲烷主要优势菌属，表明微氧条件甲烷产生主要通过乙酸脱羧途径形成。该研究为高浓度有机废水处理实现快速产甲烷提供了一种可借鉴的新工艺。

关键词: 微氧膨胀颗粒污泥床生物膜反应器, 启动, 产甲烷, 胞外聚合物, 微生物群落

CLC Number:

X703

Daling GE, Xin ZHOU, Rudy Koubode RONEL, Zeyang YIN, Wei ZHANG. Rapid start-up and microbial community characteristics of a micro-aerobic methane-producing EGSBBR system[J]. Chemical Industry and Engineering Progress, 2020, 39(12): 5203-5210.

葛大令, 周鑫, RONELRudy Koubode, 阴泽阳, 张伟. 微氧EGSBBR产甲烷系统快速启动与微生物群落特性[J]. 化工进展, 2020, 39(12): 5203-5210.

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