Synthetic pathway and synthetic strain utilized the non-grain raw material to ferment fumaric acid: a review

doi:10.16085/j.issn.1000-6613.2017-1821

Abstract

Abstract: Fumaric acid,as one of the twelve platform chemicals, is an important fine chemical product. However,the development of fumaric acid had been seriously restricted due to the imperfection of the traditional synthetic technology. Therefore, developing a new technology with high efficiency and low energy would become the new research trend in fumaric acid synthesis. In this review, the fermentation strains of fumaric acid (such as bacteria,yeasts and fungi) had been firstly introduced. Among these microbiological fermentation strains,the R. arrhizus has the highest conversion rate and yield strain of fumaric acid; and the R. oryzae is the most powerful strain of fumaric acid. Meanwhile, the synthesis pathway of fumaric acid using different non-grain materials (i.e.,glucose and xylose) and the tolerance mechanism of the R. oryzae under the furfural hydrolysate stress were illustrated clearly. Specially, the prospects and problems of fumaric acid synthesis using the synthetic strain had also been discussed in detail. Based on the above analysis, the comprehensive analysis of the inhibition mechanism of non-grain materials on fumaric acid microbiological synthesis (especially for all synthesis enzymes) will become a key work in the future. And the strain reconstruction of artificial designing would be also very important in fumaric acid synthesis for exploring the response mechanism of synthetic strain to external environment.

Key words: fumaric acid, non-grain raw material, synthetic pathway, synthetic strain

摘要： 富马酸（fumaric acid）是重要的十二种平台化合物之一及精细化工产品。近年来，传统富马酸合成工艺使用和发展受到严重制约，因而寻找低耗、高效以及环保型富马酸生产新工艺已成为研究趋势。本文介绍了微生物发酵产富马酸菌株种类，包括细菌、酵母菌、真菌。其中，真菌中的R.arrhizus是目前富马酸转化率和产量最高菌种，R.oryzae是目前富马酸生产强度最高的菌种。详细分析了以葡萄糖、木糖为底物微生物利用非粮原料发酵产富马酸的合成途径，讨论了R.oryzae耐受非粮原料水解液中糠醛胁迫的机制，重点论述了人工合成菌株发酵产富马酸的前景及存在问题。提出今后的工作重点在于全面解析非粮原料水解液中各种抑制剂对微生物发酵产富马酸全部酶类的抑制机制。同时，利用人工设计以及重构富马酸人工合成菌株，以研究合成菌株对外界环境的应答机制。

关键词: 富马酸, 非粮原料, 合成途径, 人工合成菌株

CLC Number:

Q939.97

LIN Hailong, CUI Jiaqi, WEN Jianping. Synthetic pathway and synthetic strain utilized the non-grain raw material to ferment fumaric acid: a review[J]. Chemical Industry and Engineering Progress, 2018, 37(08): 3113-3118.

林海龙, 崔佳琦, 闻建平. 非粮原料发酵富马酸的合成途径和人工合成菌株研究进展[J]. 化工进展, 2018, 37(08): 3113-3118.

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