化工进展 ›› 2019, Vol. 38 ›› Issue (11): 5074-5083.DOI: 10.16085/j.issn.1000-6613.2019-0131
收稿日期:
2019-01-21
出版日期:
2019-11-05
发布日期:
2019-11-05
通讯作者:
高文惠
作者简介:
唐录华(1993—),女,硕士研究生,研究方向为食品安全与检测。E-mail:基金资助:
Luhua TANG(),Sinan QIN,Lin GAO,Wenhui GAO()
Received:
2019-01-21
Online:
2019-11-05
Published:
2019-11-05
Contact:
Wenhui GAO
摘要:
通过自组装技术,分别以金硫键(Au—S)和氢键在羧基化多壁碳纳米管(CMWCNTs)和纳米金(AuNPs)修饰的电极表面成功组装功能单体对巯基苯胺(p-ATP)和模板分子己烯雌酚(DES),在含有p-ATP、DES、氯金酸和电聚合介质四丁基高氯酸铵的聚合液中采用电聚合的方法在组装电极表面形成聚合物膜,并用50%乙醇-0.1mol/L硫酸水溶液(1∶1,体积比)洗脱模板分子,成功制备了用于检测己烯雌酚的分子印迹电化学传感器。采用循环伏安法和差分脉冲伏安法研究印迹传感器的印迹效果和分析性能,并将该传感器应用于食品中己烯雌酚的快速检测。在最佳条件下,己烯雌酚的线性范围为1.0×10-9~1.0×10-5mol/L,检出限为3.3×10-10mol/L,样品加标平均回收率为83.46%~98.21%,相对标准偏差(RSD)在1.01%~3.74%之间(n=5)。该传感器操作简单、检测快速灵敏、成本低、抗干扰能力强、稳定性好,有重要的应用价值。
中图分类号:
唐录华,秦思楠,高林,高文惠. 基于对巯基苯胺膜的高灵敏己烯雌酚印迹传感器的制备及应用[J]. 化工进展, 2019, 38(11): 5074-5083.
Luhua TANG,Sinan QIN,Lin GAO,Wenhui GAO. Preparation and application of sensitive diethylstilbestrol imprinting sensor based on p-aminothiophenol film[J]. Chemical Industry and Engineering Progress, 2019, 38(11): 5074-5083.
样品 | 浓度/mol·L-1 | 回收率/% | 相对标准偏差/% | |||||
---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 平均值 | |||
纯牛奶 | 1.0×10-8 | 82.41 | 84.13 | 85.79 | 83.78 | 81.20 | 83.46 | 2.09 |
纯牛奶 | 1.0×10-6 | 91.15 | 97.33 | 99.20 | 95.64 | 98.83 | 96.43 | 3.39 |
猪肉 | 1.0×10-8 | 85.50 | 86.74 | 84.82 | 85.75 | 82.58 | 85.08 | 1.83 |
猪肉 | 1.0×10-6 | 99.93 | 96.67 | 98.32 | 97.84 | 97.51 | 98.05 | 1.23 |
鲜虾 | 1.0×10-8 | 83.34 | 82.71 | 84.47 | 81.63 | 85.19 | 83.47 | 1.69 |
鲜虾 | 1.0×10-6 | 90.60 | 97.86 | 98.92 | 98.27 | 99.40 | 97.01 | 3.74 |
鸡蛋 | 1.0×10-8 | 86.97 | 85.70 | 83.53 | 82.14 | 84.94 | 84.65 | 2.21 |
鸡蛋 | 1.0×10-6 | 98.82 | 96.64 | 97.94 | 99.17 | 98.51 | 98.21 | 1.01 |
表1 样品加标回收率和精密度(n=5)
样品 | 浓度/mol·L-1 | 回收率/% | 相对标准偏差/% | |||||
---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 平均值 | |||
纯牛奶 | 1.0×10-8 | 82.41 | 84.13 | 85.79 | 83.78 | 81.20 | 83.46 | 2.09 |
纯牛奶 | 1.0×10-6 | 91.15 | 97.33 | 99.20 | 95.64 | 98.83 | 96.43 | 3.39 |
猪肉 | 1.0×10-8 | 85.50 | 86.74 | 84.82 | 85.75 | 82.58 | 85.08 | 1.83 |
猪肉 | 1.0×10-6 | 99.93 | 96.67 | 98.32 | 97.84 | 97.51 | 98.05 | 1.23 |
鲜虾 | 1.0×10-8 | 83.34 | 82.71 | 84.47 | 81.63 | 85.19 | 83.47 | 1.69 |
鲜虾 | 1.0×10-6 | 90.60 | 97.86 | 98.92 | 98.27 | 99.40 | 97.01 | 3.74 |
鸡蛋 | 1.0×10-8 | 86.97 | 85.70 | 83.53 | 82.14 | 84.94 | 84.65 | 2.21 |
鸡蛋 | 1.0×10-6 | 98.82 | 96.64 | 97.94 | 99.17 | 98.51 | 98.21 | 1.01 |
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