微藻厌氧消化产甲烷：潜能、瓶颈及解决方案

doi:10.16085/j.issn.1000-6613.2018-0567

化工进展 ›› 2018, Vol. 37 ›› Issue (11): 4237-4249.DOI: 10.16085/j.issn.1000-6613.2018-0567

微藻厌氧消化产甲烷：潜能、瓶颈及解决方案

吴宇涵^1,2, 张晓然³, 胡沅胜¹

1 北京建筑大学环境与能源工程学院, 中荷未来污水处理技术研发中心, 北京 100044;
2 北控水务(中国)投资有限公司, 北京 100102;
3 北京建筑大学环境与能源工程学院, 城市雨水系统与水环境教育部重点实验室, 北京 102616

收稿日期:2018-03-21 修回日期:2018-06-17 出版日期:2018-11-05 发布日期:2018-11-05
通讯作者: 胡沅胜,副教授,硕士生导师,研究方向为污水生物处理技术、微藻生物技术、人工湿地技术。E-mail:huyuansheng@bucea.edu.cn。
作者简介:吴宇涵(1993-),女,硕士研究生,研究方向为污水处理技术、微藻生物技术。E-mail:wuyuhan129@126.com
基金资助:
国家自然科学基金（51308024）、北京建筑大学博士启动基金（ZF14047）、北京市教委青年拔尖人才培育计划（21147517010）及北京市教委科技计划一般项目（SQKM201710016016）。

Anaerobic digestion of microalgae:potential, bottleneck and solution

WU Yuhan^1,2, ZHANG Xiaoran³, HU Yuansheng¹

1 Sino-Dutch R & D Centre for Future Wastewater Treatment Technologies, School of Environment and Energy Engineering, Beijing University of Civil Engineering & Architecture, Beijing 100044, China;
2 Beijing Enterprises Water Group(China) Investment Limited, Beijing 100102, China;
3 Key Laboratory of Urban Stormwater System and Water Environment, Ministry of Education, School of Environment and Energy Engineering, Beijing University of Civil Engineering and Architecture, Beijing 102616, China

Received:2018-03-21 Revised:2018-06-17 Online:2018-11-05 Published:2018-11-05

摘要/Abstract

摘要： 厌氧消化产甲烷是实现微藻生物质能生产的重要方式，但存在消化不彻底、甲烷转化率偏低等问题。本文从理论产甲烷潜力入手，揭示了各类微藻生物质均具有良好的产甲烷潜力，普遍高于活性污泥等典型生物质，与富含能量的厨余垃圾相当。然而大部分微藻生物质的甲烷转化率都低于50%，使其实际甲烷产率处于甚至低于活性污泥的水平。微藻破壁困难和C/N比低是其甲烷转化率低的主要原因。文章从预处理和共消化两方面总结归纳了强化微藻厌氧消化的各种方法。其中低温热处理是目前最具经济技术可行性的预处理方法。与高含碳基质共消化是解决C/N比低的有效手段，但其作用需在高有机负荷下才能显现。剩余污泥不宜单独作为微藻的共消化基质。最后建议进一步探究预处理与共消化的协同作用以及重点考察连续运行工况下微藻厌氧消化的实际效果。

关键词: 微藻, 厌氧消化, 预处理, 共消化

Abstract: Anaerobic digestion of microalgae for methane productions is an important way to realize microalgal biofuels production, but there are problems such as incomplete digestion and low methane conversion ratio. By calculating the theoretical methane potential, it is revealed that all types of microalgal biomass have great methane production potential, which is generally higher than typical biomass, such as activated sludge, and is equivalent to energy-rich kitchen waste. However, the methane conversion ratio of most microalgal biomass is below 50%, making their actual methane yield even lower than that of activated sludge. Difficulty in breaking the cell wall and low C/N ratio of microalgal biomass are the main reasons for the low methane conversion ratio. Various methods for enhancing anaerobic digestion of microalgae are summarized from aspects of pretreatment and co-digestion. Among them, low-temperature thermal treatment is currently the most economically and technically feasible pretreatment method. Co-digestion with carbon-rich substrates is an effective way to solve the low C/N ratio problem, but its effect appears only at high organic loading rates. Excess sludge alone is not a suitable co-digestion substrative for microalgae. Finally, it is suggested to further explore the synergistic effect of pretreatment and co-digestion and to focus on the practical performance of anaerobic digestion of microalgae under continuous operating conditions.

Key words: microalgae, anaerobic digestion, pretreatment, co-digestion

中图分类号:

X703

吴宇涵, 张晓然, 胡沅胜. 微藻厌氧消化产甲烷：潜能、瓶颈及解决方案[J]. 化工进展, 2018, 37(11): 4237-4249.

WU Yuhan, ZHANG Xiaoran, HU Yuansheng. Anaerobic digestion of microalgae:potential, bottleneck and solution[J]. Chemical Industry and Engineering Progress, 2018, 37(11): 4237-4249.

参考文献

[1] DEBOWSKI M, ZIELINSKI M, GRALA A, et al. Algae biomass as an alternative substrate in biogas production technologies-review[J]. Renewable & Sustainable Energy Reviews, 2013, 27:596-604.
[2] U.S.DOE. National algal biofuels technology roadmap[R]. U.S. Department of Energy, Office of Energy Efficiency and Renewable Energy, Biomass Program. DOE/EE-0332. 2010.
[3] MEHRABADI A, CRAGGS R, FARID M M. Wastewater treatment high rate algal ponds (WWT HRAP) for low-cost biofuel production[J]. Bioresource Technology, 2015, 184:202-214.
[4] SIALVE B, BERNET N, BERNARD O. Anaerobic digestion of microalgae as a necessary step to make microalgal biodiesel sustainable[J]. Biotechnology Advances, 2009, 27(4):409-416.
[5] BOHUTSKYI P, BOUWER E. Biogas production from algae and cyanobacteria through anaerobic digestion:a review, analysis, and research needs[M]//Advanced Biofuels and Bioproducts. New York:Springer, 2013:873-975.
[6] MENDEZ L, MAHDY A, BALLESTEROS M, et al. Chlorella vulgaris, vs cyanobacterial biomasses:comparison in terms of biomass productivity and biogas yield[J]. Energy Conversion & Management, 2015, 92:137-142.
[7] POLAKOVICOVA G, KUSNIR P, NAGYOVA S, et al. Process integration of algae production and anaerobic digestion[C]//15th PRES Conference Process Integration, Modelling and Optimisation for Energy Saving and Pollution Reduction. 2012:1129-1134.
[8] CAPORGNO M P, TALEB A, OLKIEWICZ M, et al. Microalgae cultivation in urban wastewater:nutrient removal and biomass production for biodiesel and methane[J]. Algal Research, 2015, 10:232-239.
[9] MAHDY A, MENDEZ L, TOMÁSPEJÓ E, et al. Influence of enzymatic hydrolysis on the biochemical methane potential of Chlorella vulgaris and Scenedesmus sp.[J]. Journal of Chemical Technology & Biotechnology, 2016, 91(5):1299-1305.
[10] SCHWEDE S, REHMAN Z U, GERBER M, et al. Effects of thermal pretreatment on anaerobic digestion of Nannochloropsis salina, biomass[J]. Bioresource Technology, 2013, 143(6):505-511.
[11] BOHUTSKYI P, CHOW S, KETTER B, et al. Prospects for methane production and nutrient recycling from lipid extracted residues and whole Nannochloropsis salina using anaerobic digestion[J]. Applied Energy, 2015, 154:718-731.
[12] DARLEY W M. Biochemical composition. in:the biology of diatoms[M], (Ed.) D. Werner. Berkeley and Los Angeles, California:University of California:Press, 1997:198-217.
[13] ZHAO B S, MA J W, ZHAO Q B, et al. Efficient anaerobic digestion of whole microalgae and lipid-extracted microalgae residues for methane energy production[J]. Bioresource Technology, 2014, 161(11):423-430.
[14] ZAMALLOA C, BOON N, VERSTRAETE W. Anaerobic digestibility of Scenedesmus obliquus, and Phaeodactylum tricornutum, under mesophilic and thermophilic conditions[J]. Applied Energy, 2012, 92(2):733-738.
[15] PASSOS F, HOM-DIAZ A, BLANQUEZ P, et al. Improving biogas production from microalgae by enzymatic pretreatment[J]. Bioresource Technology, 2016, 199:347-351.
[16] DEBOWSKI M, ZIELIN′SKI M, KISIELEWSKA M, et al. Efficiency of methane fermentation of waste microalgae biomass (WMAB) collected in processes of reclamation of eutrophicated water reservoirs[J]. Environmental Earth Sciences, 2016, 75(6):1-12.
[17] GONZALEZFERNANDEZ C, SIALVE B, MOLINUEVOSALCES B. Anaerobic digestion of microalgal biomass:challenges, opportunities and research needs[J]. Bioresource Technology, 2015, 198:896.
[18] KINNUNEN V, CRAGGS R, RINTALA J. Influence of temperature and pretreatments on the anaerobic digestion of wastewater grown microalgae in a laboratory-scale accumulating-volume reactor[J]. Water Research, 2014, 57(5):247-257.
[19] ARCILA J S, BUITRÓN G. Microalgae-bacteria aggregates:effect of the hydraulic retention time on the municipal wastewater treatment, biomass settleability and methane potential[J]. Journal of Chemical Technology & Biotechnology, 2016, 91(11):2862-2870.
[20] HERNÁNDEZ D, RIAÑO B, COCA M, et al. Treatment of agro-industrial wastewater using microalgae-bacteria consortium combined with anaerobic digestion of the produced biomass[J]. Bioresource Technology, 2013, 135(2):598.
[21] ALZATE M E, MUÑOZ R, ROGALLA F, et al. Biochemical methane potential of microalgae biomass after lipid extraction[J]. Chemical Engineering Journal, 2014, 243(5):405-410.
[22] YAN L, GAO M T, HUA D L, et al. One-stage and two-stage anaerobic digestion of lipid-extracted algae[J]. Annals of Microbiology, 2015, 65(3):1465-1471.
[23] BROWNE J D, ALLEN E, MURPHY J D, et al. Evaluation of the biomethane potential from multiple waste streams for a proposed community scale anaerobic digester[J]. Environmental Technology, 2013, 34(13-16):2027.
[24] YUAN X, SHI X, ZHANG D, et al. Biogas production and microcystin biodegradation in anaerobic digestion of blue algae[J]. Energy & Environmental Science, 2011, 4(4):1511-1515.
[25] PASSOS F, FERRER I. Microalgae conversion to biogas:thermal pretreatment contribution on net energy production[J]. Environmental Science & Technology, 2014, 48(12):7171.
[26] SANTOS-BALLARDO D U, ROSSI S, REYES-MORENO C, et al. Microalgae potential as a biogas source:current status, restraints and future trends[J]. Reviews in Environmental Science & Bio/technology, 2016, 15(2):243-264.
[27] MENDEZ L, MAHDY A, TIMMERS R A, et al. Enhancing methane production of Chlorella vulgaris via thermochemical pretreatments[J]. Bioresource Technology, 2013, 149(4):136-141.
[28] GRAHAM L E, WILCOX L W. Algae[M]. Upper Saddle River, NJ:Prentice-Hall, 2000:10.
[29] GOLUEKE C G, OSWALD W J, GOTAAS H B. Anaerobic digestion of algae[J]. Applied Microbiology, 1957, 5(1):47.
[30] SÁNCHEZ HERNÁNDEZ E P, CÓRDOBA L T. Anaerobic digestion of Chlorella vulgaris for energy production[J]. Resources Conservation & Recycling, 1993, 9(1/2):127-132.
[31] RAS M, LARDON L, BRUNO S, et al. Experimental study on a coupled process of production and anaerobic digestion of Chlorella vulgaris[J]. Bioresource Technology, 2011, 102(1):200.
[32] MUSSGNUG J H, KLASSEN V, SCHLÜTER A, et al. Microalgae as substrates for fermentative biogas production in a combined biorefinery concept[J]. Journal of Biotechnology, 2010, 150(1):51-56.
[33] MONTINGELLI M E, TEDESCO S, OLABI A G. Biogas production from algal biomass:a review[J]. Renewable & Sustainable Energy Reviews, 2015, 43:961-972.
[34] ZHONG W, CHI L, LUO Y, et al. Enhanced methane production from Taihu Lake blue algae by anaerobic co-digestion with corn straw in continuous feed digesters[J]. Bioresource Technology, 2013, 134(2):264.
[35] KINNUNEN H V, KOSKINEN P E, RINTALA J. Mesophilic and thermophilic anaerobic laboratory-scale digestion of Nannochloropsis microalga residues[J]. Bioresource Technology, 2014, 155(2):314-322.
[36] HERRMANN C, KALITA N, WALL D, et al. Optimised biogas production from microalgae through co-digestion with carbon-rich co-substrates[J]. Bioresource Technology, 2016, 214:328-337.
[37] PASSOS F, GARCÍA J, FERRER I. Impact of low temperature pretreatment on the anaerobic digestion of microalgal biomass[J]. Bioresource Technology, 2013, 138(2):79-86.
[38] BOHUTSKYI P, BETENBAUGH M J, BOUWER E J. The effects of alternative pretreatment strategies on anaerobic digestion and methane production from different algal strains[J]. Bioresource Technology, 2014, 155(10):366.
[39] XUE B, LANT P A, JENSEN P D, et al. Enhanced methane production from algal digestion using free nitrous acid pre-treatment[J]. Renewable Energy, 2016, 88:383-390.
[40] PRAJAPATI S K, MALIK A, VIJAY V K, et al. Enhanced methane production from algal biomass through short duration enzymatic pretreatment and codigestion with carbon rich waste[J]. RSC Advances, 2015, 5(82):67175-67183.
[41] MAHDY A, MENDEZ L, BALLESTEROS M, et al. Enhanced methane production of Chlorella vulgaris, and Chlamydomonas reinhardtii by hydrolytic enzymes addition[J]. Energy Conversion & Management, 2014, 85(85):551-557.
[42] CARRÈRE H, DUMAS C, BATTIMELLI A, et al. Pretreatment methods to improve sludge anaerobic degradability:a review[J]. Journal of Hazardous Materials, 2010, 183(1-3):1-15.
[43] ALZATE M E, MUÑOZ R, ROGALLA F, et al. Biochemical methane potential of microalgae:influence of substrate to inoculum ratio, biomass concentration and pretreatment[J]. Bioresource Technology, 2012, 123(123):488.
[44] LIU C Z, WANG F, STILES A R, et al. Ionic liquids for biofuel production:opportunities and challenges[J]. Applied Energy, 2012, 92(4):406-414.
[45] ZHOU N, ZHANG Y M, GONG X W, et al. Ionic liquids-based hydrolysis of Chlorella biomass for fermentable sugars[J]. Bioresource Technology, 2012, 118(8):512-517.
[46] JANKOWSKA E, SAHU A K, OLESKOWICZ-POPIEL P. Biogas from microalgae:review on microalgae's cultivation, harvesting and pretreatment for anaerobic digestion[J]. Renewable & Sustainable Energy Reviews, 2016, 75:692-709.
[47] RODRIGUEZ C, ALASWAD A, MOONEY J, et al. Pre-treatment techniques used for anaerobic digestion of algae[J]. Fuel Processing Technology, 2015, 138:765-779.
[48] MARKOU G, ANGELIDAKI I, GEORGAKAKIS D. Carbohydrate- enriched cyanobacterial biomass as feedstock for bio-methane production through anaerobic digestion[J]. Fuel, 2013, 111(9):872-879.
[49] KLASSEN V, BLIFERNEZKLASSEN O, HOEKZEMA Y, et al. A novel one-stage cultivation/fermentation strategy for improved biogas production with microalgal biomass[J]. Journal of Biotechnology, 2015, 215:44-51.
[50] FERNÁNDEZ-RODRÍGUEZ M J, RINCÓN B, FERMOSO F G, et al. Assessment of two-phase olive mill solid waste and microalgae co-digestion to improve methane production and process kinetics[J]. Bioresource Technology, 2014, 157(4):263-269.
[51] YEN H-W, BRUNE D. Anaerobic co-digestion of algal sludge and waste paper to produce methane[J]. Bioresource Technology, 2007, 98(1):130-134.
[52] ZHEN G, LU X, KOBAYASHI T, et al. Anaerobic co-digestion on improving methane production from mixed microalgae (Scenedesmus sp. Chlorella sp.) and food waste:kinetic modeling and synergistic impact evaluation[J]. Chemical Engineering Journal, 2016, 299:332-341.
[53] SOLÉ-BUNDÓ M, ESKICIOGLU C, GARFÍ M, et al. Anaerobic co-digestion of microalgal biomass and wheat straw with and without thermo-alkaline pretreatment[J]. Bioresource Technology, 2017, 237:89-98.
[54] WANG M, SAHU A K, RUSTEN B, et al. Anaerobic co-digestion of microalgae Chlorella sp. and waste activated sludge[J]. Bioresource Technology, 2013, 142(8):585.
[55] WANG M, PARK C. Investigation of anaerobic digestion of Chlorella, sp. and Micractinium, sp. grown in high-nitrogen wastewater and their co-digestion with waste activated sludge[J]. Biomass & Bioenergy, 2015, 80:30-37.
[56] CAPORGNO M P, TROBAJO R, CAIOLA N, et al. Biogas production from sewage sludge and microalgae co-digestion under mesophilic and thermophilic conditions[J]. Renewable Energy, 2015, 75:374-380.
[57] MAHDY A, MENDEZ L, BALLESTEROS M, et al. Algaculture integration in conventional wastewater treatment plants:anaerobic digestion comparison of primary and secondary sludge with microalgae biomass[J]. Bioresource Technology, 2015, 184:236-244.

微藻厌氧消化产甲烷：潜能、瓶颈及解决方案

Anaerobic digestion of microalgae:potential, bottleneck and solution

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李由, 吴越, 钟禹, 林琦璇, 任俊莉. 酸性熔盐水合物预处理麦秆高效制备木糖及其对酶解效率的影响[J]. 化工进展, 2023, 42(9): 4974-4983.
[2]	奚永兰, 王成成, 叶小梅, 刘洋, 贾昭炎, 曹春晖, 韩挺, 张应鹏, 田雨. 微纳米气泡在厌氧消化中的应用研究进展[J]. 化工进展, 2023, 42(8): 4414-4423.
[3]	庄捷, 薛锦辉, 赵斌成, 张文艺. 猪粪厌氧消化进程中重金属与腐殖质的有机结合机制[J]. 化工进展, 2023, 42(6): 3281-3291.
[4]	张乐乐, 钱运东, 朱华曈, 冯思龙, 杨秀娜, 于颖, 杨强, 卢浩. 加氢原料煤焦油脱水除盐预处理工艺优化限值[J]. 化工进展, 2023, 42(5): 2298-2305.
[5]	孟晓山, 汤子健, 陈琳, 呼和涛力, 周政忠. 厌氧消化系统酸化预警及调控技术研究进展[J]. 化工进展, 2023, 42(3): 1595-1605.
[6]	祝佳欣, 朱雯喆, 徐俊, 谢靖, 王文标, 谢丽. 基于导电材料强化抗生素胁迫厌氧消化的研究进展[J]. 化工进展, 2023, 42(2): 1008-1019.
[7]	秦振芳, 廖日红, 马伟芳. 吸收-微藻法固定燃气电厂低浓度CO₂同步产油技术研究进展[J]. 化工进展, 2023, 42(1): 94-106.
[8]	刘亚利, 张宏伟, 康晓荣. 微塑料对污泥厌氧消化的影响和机理[J]. 化工进展, 2022, 41(9): 5037-5046.
[9]	韩明阳, 乔慧, 付佳铭, 马泽雯, 王妍, 欧阳嘉. 非水溶剂预处理木质纤维原料研究进展[J]. 化工进展, 2022, 41(8): 4086-4097.
[10]	郑小梅, 林茹晶, 周文静, 徐泠, 张洪宁, 张昕颖, 谢丽. 微生物电解池辅助CO₂甲烷化阴极材料的研究进展[J]. 化工进展, 2022, 41(5): 2476-2486.
[11]	邵明帅, 张超, 吴华南, 王宁, 陈钦冬, 徐期勇. 水热耦合厌氧消化技术处理餐厨垃圾沼渣沼液及工艺能耗分析[J]. 化工进展, 2022, 41(5): 2733-2742.
[12]	解先利, 刘云云, 余强, 张宇, 张荣清, 邱雨心. 低共熔溶剂预处理提高甘草渣酶解效果优化[J]. 化工进展, 2022, 41(3): 1349-1356.
[13]	李海涛, 汪东. 精对苯二甲酸生产废水处理与CO₂协同利用技术的实践与展望[J]. 化工进展, 2022, 41(3): 1132-1135.
[14]	阮敏, 孙宇桐, 黄忠良, 李辉, 张轩, 吴希锴, 赵成, 姚世蓉, 张拴保, 张巍, 黄兢. 污泥预处理-厌氧消化体系的能源经济性评价[J]. 化工进展, 2022, 41(3): 1503-1516.
[15]	王娜, 宋秀兰, 昝博韬. 复合菌群利用模拟APG协同FNA预处理剩余污泥水解液合成PHA[J]. 化工进展, 2022, 41(2): 1017-1024.