非粮原料发酵富马酸的合成途径和人工合成菌株研究进展

doi:10.16085/j.issn.1000-6613.2017-1821

化工进展 ›› 2018, Vol. 37 ›› Issue (08): 3113-3118.DOI: 10.16085/j.issn.1000-6613.2017-1821

非粮原料发酵富马酸的合成途径和人工合成菌株研究进展

林海龙¹, 崔佳琦², 闻建平²

1 国投生物科技投资有限公司, 北京 100034;
2 天津大学化工学院, 天津 300350

收稿日期:2017-08-31 修回日期:2017-11-10 出版日期:2018-08-05 发布日期:2018-08-05
通讯作者: 闻建平,教授,研究方向为生物反应工程。
作者简介:林海龙(1976-),男,博士,高级工程师。
基金资助:
国家高技术研究发展计划项目（2011AA02A206）。

Synthetic pathway and synthetic strain utilized the non-grain raw material to ferment fumaric acid: a review

LIN Hailong¹, CUI Jiaqi², WEN Jianping²

1 Guotou Biotechnology Investment Co., Ltd., Beijing 100034, China;
2 School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China

Received:2017-08-31 Revised:2017-11-10 Online:2018-08-05 Published:2018-08-05

摘要/Abstract

摘要： 富马酸（fumaric acid）是重要的十二种平台化合物之一及精细化工产品。近年来，传统富马酸合成工艺使用和发展受到严重制约，因而寻找低耗、高效以及环保型富马酸生产新工艺已成为研究趋势。本文介绍了微生物发酵产富马酸菌株种类，包括细菌、酵母菌、真菌。其中，真菌中的R.arrhizus是目前富马酸转化率和产量最高菌种，R.oryzae是目前富马酸生产强度最高的菌种。详细分析了以葡萄糖、木糖为底物微生物利用非粮原料发酵产富马酸的合成途径，讨论了R.oryzae耐受非粮原料水解液中糠醛胁迫的机制，重点论述了人工合成菌株发酵产富马酸的前景及存在问题。提出今后的工作重点在于全面解析非粮原料水解液中各种抑制剂对微生物发酵产富马酸全部酶类的抑制机制。同时，利用人工设计以及重构富马酸人工合成菌株，以研究合成菌株对外界环境的应答机制。

关键词: 富马酸, 非粮原料, 合成途径, 人工合成菌株

Abstract: Fumaric acid,as one of the twelve platform chemicals, is an important fine chemical product. However,the development of fumaric acid had been seriously restricted due to the imperfection of the traditional synthetic technology. Therefore, developing a new technology with high efficiency and low energy would become the new research trend in fumaric acid synthesis. In this review, the fermentation strains of fumaric acid (such as bacteria,yeasts and fungi) had been firstly introduced. Among these microbiological fermentation strains,the R. arrhizus has the highest conversion rate and yield strain of fumaric acid; and the R. oryzae is the most powerful strain of fumaric acid. Meanwhile, the synthesis pathway of fumaric acid using different non-grain materials (i.e.,glucose and xylose) and the tolerance mechanism of the R. oryzae under the furfural hydrolysate stress were illustrated clearly. Specially, the prospects and problems of fumaric acid synthesis using the synthetic strain had also been discussed in detail. Based on the above analysis, the comprehensive analysis of the inhibition mechanism of non-grain materials on fumaric acid microbiological synthesis (especially for all synthesis enzymes) will become a key work in the future. And the strain reconstruction of artificial designing would be also very important in fumaric acid synthesis for exploring the response mechanism of synthetic strain to external environment.

Key words: fumaric acid, non-grain raw material, synthetic pathway, synthetic strain

中图分类号:

Q939.97

林海龙, 崔佳琦, 闻建平. 非粮原料发酵富马酸的合成途径和人工合成菌株研究进展[J]. 化工进展, 2018, 37(08): 3113-3118.

LIN Hailong, CUI Jiaqi, WEN Jianping. Synthetic pathway and synthetic strain utilized the non-grain raw material to ferment fumaric acid: a review[J]. Chemical Industry and Engineering Progress, 2018, 37(08): 3113-3118.

参考文献

[1] ENGEL C A, STRAATHOF A J, ZIJLMANS T W, et al. Fumaric acid production by fermentation[J]. Applied Microbiology and Biotechnology, 2008, 78(3):379-389.
[2] SAUER M, PORRO D, MATTANOVICH D, et al. Microbial production of organic acids:expanding the markets[J]. Trends in Biotechnology, 2008, 26(2):100-108.
[3] AIKATERINI P, NIKOLAOS A, MARIA P, et al. Biotechnological production of fumaric acid:the effect of morphology of Rhizopus arrhizus NRRL 2582[J]. Fermentation, 2017, 3(3):33.
[4] ICHIKAWA S, ⅡNO T, SATO S, et al. Improvement of production rate and yield of fumaric acid from maleic acid by heat treatment of Pseudomonas alcaligenes strain XD-1[J]. Biochemical Engineering Journal, 2003, 13(1):7-13.
[5] ZHANG K, YU C, YANG S T. Effects of soybean meal hydrolysate as the nitrogen source on seed culture morphology and fumaric acid production by Rhizopus oryzae[J]. Process Biochemistry, 2015, 50(2):173-179.
[6] DING Y Y, LI S, DOU C, et al.Production of fumaric acid by Rhizopus oryzae:role of carbon-nitrogen ratio[J]. Applied Biochemistry and Biotechnology, 2011, 164(8):1461-1467.
[7] BAEYENS J, KANG Q, APPELS L, et al. Challenges and opportunities in improving the production of bio-ethanol[J]. Progress in Energy and Combustion science, 2015, 47:60-88.
[8] KNAUF M, MONIRUZZAMAN M. Lignocellulosic biomass processing:a perspective[J]. International Sugar Journal, 2004, 106(1263):147-150.
[9] WANG H T, PAN L J, LIN X J, et al. Study on the fermentation of fumaric acid by Rhizopus oryzae[J]. Research Journal of Biotechnology, 2014, 9(11):62-71.
[10] WATKINS D W, JENKINS J M, GRAYSON K J, et al. Construction and in vivo assembly of a catalytically proficient and hyperthermostable de novo enzyme[J]. Nature Communicatioin, 2017, 25(1):358.
[11] LIAO W, LIU Y, FREAR C, et al. A new approach of pellet formation of a filamentous fungus Rhizopus oryzae[J]. Bioresource Technology, 2007,98(18):3415-3423.
[12] TAMAKAWA H, IKUSHIMA S, YOSHIDA S, et al. Efficient production of L-lactic acid from xylose by a recombinant Candida utilisstrain[J]. Journal of Bioscience and Bioengineering, 2012, 113(1):73-75.
[13] BAI D M, ZHAO X M, LI X G, et al. Strain improvement of Rhizopus oryzae for over-production of L(+)-lactic acid and metabolic flux analysis of mutants[J]. Biochemical Engineering Journal, 2004, 18(1):41-48.
[14] PETRUCCIOLI M, ANGIANI E, FEDERICI F, et al. Semi-continuous fumaric acid production by Rhizopus arrhizus immobilized in polyurethane sponge[J]. Process Biochemistry, 1996, 31(5):463-469.
[15] CARTA F, SOCCOL C, RAMOS L, et al. Production of fumaric acid by fermentation of enzymatic hydrolysates derived from cassava bagasse[J]. Bioresource Technology, 1999, 68(1):23-28.
[16] 王月皎,夏乾竣,李迅,等. 解糖热纤维菌木糖苷酶的分离纯化及其酶学性质[J]. 化工进展,2017,36(3):1041-1046. WANG Y J, XIA Q J, LI X, et al. Purification and characterization of a thermostable β-xylosidase from Caldicellulosiruptor saccharolyticus[J]. Chemical Industry and Engineering Progress, 2017, 36(3):1041-1046.
[17] PRONK J T, STEENSMA H, VAN-DIJKEN J P. Pyruvate metabolism in Saccharomyces cerevisiae[J]. Yeast, 1996, 12(16):1607-1633.
[18] ADEBOYE P T, BETTIGA M, OLSSON L, et al. The chemical nature of phenolic compounds determines their toxicity and induces distinct physiological responses in Saccharomyces cerevisiae in lignocellulose hydrolysates[J]. Applied Microbiology and Biotechnology, 2014, 4(1):46-52.
[19] FAZELI N S, BRANDBERG T, LENNARTSSON P R, et al. Inhibitor tolerance:a comparison between Rhizopus sp. and Saccharomyces cerevisiae[J]. BioResources, 2013, 8(4):5524-5535.
[20] OSMANI S A, SCRUTTON M C. The sub-cellular localisation and regulatory properties of pyruvate carboxylase from Rhizopus arrhizus[J]. European Journal of Biochemistry, 1985,147(1):119-128.
[21] 刘维喜, 付晶, 章博, 等. 微生物木糖代谢途径改造制备生物基化学品[J]. 生物工程学报, 2013, 29(8):1161-1172. LIU W X, FU J, ZHANG B, et al. Engineering of the xylose metabolic pathway for microbial production of bio-based chemicals[J]. Chinese Journalof Biotechnology, 2013, 29(8):1161-1172.
[22] CHEN X L, WU J, SONG W, et al. Fumaric acid production by Torulopsis glabrata:engineering the urea cycle and the purine nucleotide cycle[J]. Biotechnology and Bioengineering, 2015, 112(1):156-167.
[23] PAN X R, LIU H, WEN J P, et al. Omics-based approaches reveal phospholipids remodeling of Rhizopus oryzae responding to furfural stress for fumaric acid-production from xylose[J]. Bioresource Technology, 2016, 222:24-32.
[24] CASINI A, STORCH M, BALDWIN G S, et al. Bricks and blueprints:methods and standards for DNA assembly[J]. Nature Reviews Molecular Cell Biology, 2015, 16(9):568-576.
[25] CAO Y L, RYSER M D, PAYNE S, et al. Collective space-sensing coordinates pattern scaling in engineered bacteria[J]. Cell, 2016, 165(3):620-630.
[26] YAMAICHI Y, DORR T. Transposon insertion site sequencing for synthetic lethal screening[J]. Methods in Molecular Biology, 2017, 1624:39-49.
[27] HAGEMANN M, HESS W R. Systems and synthetic biology for the biotechnological application of cyanobacteria[J]. Current Opinion in Biotechnology, 2017, 49:94-99.
[28] LI S Y, ZHAO G P, WANG J. C-brick:a new standard for assembly of biological parts using Cpf1[J]. ACS Synthetic Biology, 2016, 5(12). 1383-1388.
[29] KOSURI S, CHURCH G M. Large-scale de novo DNA synthesis:technologies and applications[J]. Nat. Methods, 2014, 11(5):499-507.
[30] XU G Q, ZOU W, CHEN X L, et al. Fumaric acid production in Saccharomyces cerevisiae by in silico aided metabolic engineering[J]. PLoS One, 2012, 7(12):e52086.
[31] XU G, LIU L, CHEN J, et al. Reconstruction of cytosolic fumaric acid biosynthetic pathways in Saccharomyces cerevisiae[J]. Microbial Cell Factory, 2012, 11(1):24-31.
[32] XU G Q, CHEN X L, LIU L M, et al. Fumaric acid production in Saccharomyces cerevisiae by simultaneous use of oxidative and reductive routes[J]. Bioresource Technology, 2013, 148(8):91-96.
[33] SONG C W, KIM D I, LEE S Y, et al. Metabolic engineering of Escherichia coli for the production of fumaric acid[J]. Biotechnology and Bioengineering, 2013, 110(7):2025-2034.
[34] LI N, ZHANG B, WANG Z W, et al. Engineering Escherichia coli for fumaric acid production from glycerol[J]. Bioresource Technology, 2014, 174:81-87.
[35] WEI L, QI H S, WEN J P, et al. Engineering Scheffersomyces stipitis for fumaric acid production from xylose[J]. Bioresource Technology, 2015, 187:246-254.

非粮原料发酵富马酸的合成途径和人工合成菌株研究进展

Synthetic pathway and synthetic strain utilized the non-grain raw material to ferment fumaric acid: a review

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价

[1]	赵鑫, 熊健力, 任叶琳, 杨家鑫, 李伟, 韩雪容. 细菌纤维素合成与鉴定研究综述[J]. 化工进展, 2020, 39(S2): 262-268.
[2]	于新磊, 毛雨丰, 张晓霞, 陆凌雪, 王智文, 陈涛. 生物法生产3-羟基丙酸研究进展[J]. 化工进展, 2018, 37(11): 4427-4436.
[3]	张昆，高振，李霜，陈健，付永前，黄和，韦萍. 米根霉在5L发酵罐种子培养过程中的形态控制 [J]. 化工进展, 2008, 27(12): 1967-.