细菌纤维素合成与鉴定研究综述

doi:10.16085/j.issn.1000-6613.2020-0384

摘要/Abstract

摘要：

细菌纤维素（BC）因其独特性能被广泛应用于医药、食品等领域，目前其高产量菌株筛选、合成成本降低及合成途径改良等成为研究热点。本文依据国内外文献并结合团队研究成果对BC合成与鉴定的相关研究进行综述。首先对BC合成菌筛选及碳源利用的研究进行了分析，总结了降低BC合成成本的研究思路。其次对鉴定菌株合成产物的方法进行了归纳，总结了不同方法的特点。然后结合本团队筛选出的BC生产菌XJL-06-4 BC合成酶基因分析结果，综述了BC合成途径、合成酶存在形式以及基因水平调控作用，为BC在分子水平上通过改变合成途径提高产量提供新思路。最后，总结BC微生物发酵生产存在的问题，多角度提出解决方案。

关键词: 细菌纤维素, 碳源利用, 产物鉴定, 合成途径, 合成酶

Abstract:

Bacterial cellulose (BC) has been widely used in medicine, food and other fields because of its unique properties. Based on the domestic and foreign literature and team research results, this paper reviews the related research on BC synthesis and identification. Firstly, the screening of BC synthetic bacteria and the utilization of carbon source were analyzed, and the research ideas of reducing BC synthesis cost were summarized. Secondly, the methods to identify the synthetic products of strains were summarized, and the characteristics of different methods were summarized. Then combined with the team selected BC production bacteria XJL-06-4BC synthesis The results of enzyme gene analysis reviewed the BC synthesis pathway, the form of synthetase and the regulation of gene level, a new idea for BC to increase yield by changing the synthesis pathway at the molecular level. Finally, summarize the problems existing in BC microbial fermentation production, and put forward solutions from many angles.

Key words: bacterial cellulose, carbon source utilization, product identification, synthetic pathway, synthase

中图分类号:

赵鑫, 熊健力, 任叶琳, 杨家鑫, 李伟, 韩雪容. 细菌纤维素合成与鉴定研究综述[J]. 化工进展, 2020, 39(S2): 262-268.

Xin ZHAO, Jianli XIONG, Yelin REN, Jiaxin YANG, Wei LI, Xuerong HAN. Synthesis and identification of bacterial cellulose[J]. Chemical Industry and Engineering Progress, 2020, 39(S2): 262-268.

图/表 5

图1 细菌纤维素膜的SEM图

图2 细菌纤维素的FTIR谱图

图3 6株菌产膜的XRD图

图4 NEDO-01和ATCC23769合成产物的DD/MAS 13C-NMR光谱图

表1 菌株XJL-06-4纤维素合成酶的主要信息

名称	起始位点	结束位点	主要作用
bcsQ	2321	3403	细胞定位、细胞间黏附
bcsZ	19944	21065	调控纤维素的合成

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