关键词: 木质纤维素, 预处理, 酶解, 组成结构, 分析表征

Abstract: A complex chemical composition and recalcitrant heterogenic architecture of lignocellulosic biomass feedstock makes analysis and detection difficult. Meanwhile the situation is aggravated due to substrate diversity from different species pretreated by different methods. According to extensive literatures，modern analysis technique characterizing the composition-structure of native plant cell wall and its change after pretreatment，as well as conformation of substrate to enzyme has become an increasingly important tool for elucidating mechanisms of lignocellulose-to-sugar conversion. In this paper，several common analytic techniques（SEM，FT-IR，XRD，NMR） characterizing changes of various lignocellulosic cell wall architectures modified by different pretreatments were reviewed. Then recent emerging techniques in this field （AFM，in-suit AFM，multi-scale visualization，correlative imagining in real time） were discussed. Then conclusions based on the present progress of characterization technique applied in dissecting lignocellulosic cell wall architectures were made. Future work in improving lignocellulose-to-sugar conversion was suggested，which gave a new insight to enhance the efficiency and reduce the cost of pretreatment and enzymatic hydrolysis for lignocellulosic biorefinery.

Key words: lignocelluloses, pretreatment, enzymatic hydrolysis, composition and structure, analytical characterization