木质素对蔗渣酶水解的影响

doi:10.16085/j.issn.1000-6613.2016.12.047

化工进展 ›› 2016, Vol. 35 ›› Issue (12): 4072-4075.DOI: 10.16085/j.issn.1000-6613.2016.12.047

木质素对蔗渣酶水解的影响

肖芳¹, 覃益民¹, 刘幽燕^1,2

1 广西大学化学化工学院, 广西南宁 530004;
2 广西生物炼制重点实验室, 广西南宁 530004

收稿日期:2016-05-09 修回日期:2016-05-24 出版日期:2016-12-05 发布日期:2016-12-05
通讯作者: 覃益民,博士,教授。E-mail:qym6289@sina.com。
作者简介:肖芳(1989-),女,硕士研究生。E-mail:quitefang@sina.com。
基金资助:
国家自然科学基金（21276053）及广西生物炼制重点实验室培育基地开放课题项目。

The effect of lignin in bagasse enzyme hydrolysis

XIAO Fang¹, QIN Yimin¹, LIU Youyan^1,2

1 School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China;
2 Guangxi Key Laboratory of Biorefinery, Nanning 530004, Guangxi, China

Received:2016-05-09 Revised:2016-05-24 Online:2016-12-05 Published:2016-12-05

摘要/Abstract

摘要： 采用机械活化方法对蔗渣进行处理，研究在原料可及性变化时木质素对蔗渣酶解的影响，用牛血清蛋白（BSA）预先使原料中木质素吸附饱和的方法来测定木质素对酶的吸附情况、用X-射线衍射和扫描电镜来测定蔗渣结晶结构、表面形态来表征原料可及性，从而分析影响的可能机制。结果表明木质素对蔗渣酶水解的影响与蔗渣可及性有关，原料可及性越高，影响越小。当蔗渣可及性相对较低时，此时木质素影响蔗渣酶水解转化率的两种方式（木质素与纤维素、半纤维素组成的致密结构限制纤维素对酶的可及性及木质素对纤维素酶的无效吸附作用）同时存在。当原料的可及性增加到一定程度后（如机械活化2h的蔗渣），木质素对纤维素酶的吸附作用几乎消除，木质素对蔗渣酶水解的影响主要表现为对酶的可及性的限制。

关键词: 生物质, 木质素, 水解, 纤维素可及性, 机械活化

Abstract: The mechanical activation method was employed to process bagasse. The effect of lignin on enzymatic hydrolysis was studied effect when the raw material accessibility changed. The X-ray diffraction and scanning electron microscopy(SEM)were used to determine the crystal structure and surface morphology of bagasse,to characterize the raw material accessibility,and analyze the influence of the possible mechanisms. The results showed that the effect of lignin on bagasse enzyme hydrolysis was related to bagasse accessibility. The higher raw material availability was,the smaller the effect was. When the accessibility of bagasse was relatively low,lignin affected the rate of enzymatic hydrolysis of bagasse in two ways (limiting cellulose accessibility and unproductive binding of the enzymes) simultaneously. When the availability of raw material increased to a certain level (for example,2 hours by mechanical activation of bagasse) and lignin adsorption of cellulose was virtually eliminated. The main effect of Lignin on bagasse enzymatic hydrolysis was limiting enzyme accessibility.

Key words: biomass, lignin, hydrolysis, cellulose accessibility, mechanical activation

中图分类号:

TQ353.4⁺23

肖芳, 覃益民, 刘幽燕. 木质素对蔗渣酶水解的影响[J]. 化工进展, 2016, 35(12): 4072-4075.

XIAO Fang, QIN Yimin, LIU Youyan. The effect of lignin in bagasse enzyme hydrolysis[J]. Chemical Industry and Engineering Progree, 2016, 35(12): 4072-4075.

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木质素对蔗渣酶水解的影响

The effect of lignin in bagasse enzyme hydrolysis

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