化工进展 ›› 2023, Vol. 42 ›› Issue (10): 5390-5398.DOI: 10.16085/j.issn.1000-6613.2022-2032

• 生物与医药化工 • 上一篇    下一篇

pH响应性磁性介孔纳米载药系统的制备

刘慧慧(), 史笑飞, 王倩男, 刘锦博, 张静()   

  1. 郑州大学化工学院,河南 郑州 450001
  • 收稿日期:2022-11-01 修回日期:2023-02-01 出版日期:2023-10-15 发布日期:2023-11-11
  • 通讯作者: 张静
  • 作者简介:刘慧慧(1998—),女,硕士研究生,研究方向为磁性纳米粒子载药。E-mail:hui15981979015@163.com

Preparation of pH responsive magnetic mesoporous nanoparticle drug loading system

LIU Huihui(), SHI Xiaofei, WANG Qiannan, LIU Jinbo, ZHANG Jing()   

  1. College of Chemical Engineering, Zhengzhou University, Zhengzhou 450001, Henan, China
  • Received:2022-11-01 Revised:2023-02-01 Online:2023-10-15 Published:2023-11-11
  • Contact: ZHANG Jing

摘要:

研究开发了一种基于磁性介孔二氧化硅和聚多巴胺(PDA)混合体的智能给药平台,可用于pH响应性给药。通过共沉淀法制备Fe3O4,以十六烷基三甲基溴化铵(CTAB)为模板,硅酸四乙酯(TEOS)为硅源,通过Stober法获得了磁性纳米材料Fe3O4@mSiO2。负载抗癌药物阿霉素(DOX)后,通过碱性条件下多巴胺的氧化自聚合,在其表面形成pH敏感的PDA涂层,得到DOX/Fe3O4@mSiO2@PDA磁性纳米载药系统。通过透射电子显微镜、X射线衍射、傅里叶红外光谱仪、比表面积测试和振动样品磁强计等技术手段对材料进行表征。结果表明Fe3O4@mSiO2的形貌为球形,拥有良好介孔结构和高达619.16m2/g的比表面积;VSM表明载体Fe3O4@mSiO2@PDA具有良好的磁性。当载药过程中DOX浓度为0.5mg/mL、温度为37℃,反应36h后,载药率为51.69%,包封率为82.70%。释药曲线表明纳米载药系统具有pH响应性和药物缓释的特点;噻唑蓝比色法(MTT法)细胞毒性实验表明Fe3O4@mSiO2@PDA生物相容性良好,DOX/Fe3O4@mSiO2@PDA有良好的抗肿瘤效果。该纳米载药系统在药物靶向递送中具有潜在的应用前景。

关键词: 四氧化三铁, 磁性纳米材料, 介孔, pH响应性, 药物递送, 阿霉素

Abstract:

We developed an intelligent drug delivery platform based on the mixture of magnetic mesoporous silica and polydopamine (PDA), which can be used for pH responsive drug delivery. Magnetic cores Fe3O4 was prepared by co-precipitation method. The magnetic nanomaterial Fe3O4@mSiO2 was obtained by the Stober method using cetyltrimethylammonium bromide (CTAB) as a template and tetraethyl silicate (TEOS) as a silicon source. Anticancer drug doxorubicin (DOX) was used as the model drug to be encapsulated into Fe3O4@mSiO2. The surface of the carrier was modified with pH-sensitive PDA coating by oxidation self-polymerization of dopamine at alkaline pH to obtain the magnetic nano drug loading system DOX/Fe3O4@mSiO2@PDA. The materials were characterized by Transmission electron microscope, X-ray diffraction, Fourier Transform infrared spectrometer, Brunauer Emmett Teller and vibration sample magnetometer. We found that Fe3O4@mSiO2 was spherical with good mesoporous structure and a specific surface area of up to 619.16m2/g. VSM indicated that the carrier Fe3O4@mSiO2@PDA had good magnetic properties. During the drug loading process, when DOX concentration was 0.5mg/mL, temperature was 37℃, and reaction time was 36h, the drug loading rate and encapsulation rate were up to 51.69% and 82.70%, respectively. The drug release curve showed that the nano drug delivery system had the characteristics of pH responsiveness and slow drug release. Thiazole blue colorimetric assay (MTT) cytotoxicity showed that Fe3O4@mSiO2@PDA had good biocompatibility, and DOX/Fe3O4@mSiO2@PDA had obvious inhibitory effect on cancer cells. The nano drug delivery system has a potential application prospect in targeted drug delivery.

Key words: ferric oxide, magnetic nanomaterials, mesoporous, pH responsiveness, drug delivery, doxorubicin (DOX)

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