关键词: 生物技术, 蛋白, 溶解性, 融合标签, 重组蛋白质, 可溶性表达, 纯化

Abstract: Fusion tags were effective tools for large-scale production of soluble proteins. Application of fusion tags in soluble expression has become a priority of research. This paper mainly introduced the most frequently used fusion tags，as well as the possible mechanism of their ability to enhance solubility. Five models have been employed to explain the role of fusion tags in protein folding from different aspects. Most powerful fusion tags，such as MBP and NusA，act as a general molecular chaperone to assist passenger protein folding into native conformation. Furthermore，it also provides strategies to remove fusion tags and the efficiency of proteases. At last，it elaborates their prospects and challenges faced in large-scale production of protein. This review serves as an excellent reference for those working on protein fusion tags. In view of the limitation of existing tags，our laboratory is developing a novel multifunctional fusion tag. Present study has showed that it can greatly improve soluble expression level，and further study is conducted on the purification methods and mechanism of it.

Key words: biotechnology, protein, solubility, fusion tag, recombinant protein, solubility, purification