Abstract: Essential oils refer to the active components from natural products. The selective extraction and separation of essential oils is of great importance. In this paper, the extraction and separation of essential oils using supercritical fluids was reviewed. The opitmal methods of extraction by using pure supercritical fluid, addtion of co-solvent and the combination of supercritical extraction with other separation technologies such as distillation, adsorption, molecular distillation, double retrograde vaporization, together with the application were also discussed.