电纺直写制备聚己内酯支架形貌及精度

doi:10.16085/j.issn.1000-6613.2018-1623

摘要/Abstract

摘要：

作为一种新型的微纳制造技术，熔体直写电纺被广泛用于组织工程支架的可控制备,有序的纤维沉积是该领域应用的前提条件。对于支架成型精度的探究，本文使用生物可降解材料聚己内酯（PCL），采用自行设计的熔体电纺三维可控成型设备进行实验，考察了纤维间距对二维并行纤维沉积形貌及成型精度的影响，以及纺丝电压和网格大小对三维网格结构形貌及精度影响。结果表明，随着并行纤维设定距离的增大，纤维的沉积误差减小，并最终趋于平稳。对于三维网格结构，随电压的增加，最大沉积层数量先增大后减小，当纺丝电压为6kV时达到最大沉积层数15层。成型精度误差先减小后增大，当纺丝电压为7kV时，精度最高误差小于5%。随设定网格边长的增大，沉积层数不断增大。成型精度逐渐提高，当网格边长大于等于1.5mm时，沉积误差趋于稳定，并维持在5%左右。

关键词: 直写电纺, 聚己内酯, 形貌, 成型精度, 组织工程学

Abstract:

As a new micro-nano manufacturing technology, melt direct-write electrospinning is widely used for the controllable preparation of tissue engineering scaffolds, and ordered fiber deposition is a prerequisite for application in this field. For the exploration of stent forming precision, the biodegradable material polycaprolactone (PCL) was used, and the self-designed melt electrospinning three-dimensional controllable molding equipment was applied for the experiment. The effects of fiber spacing on the morphology and molding accuracy of two-dimensional parallel fiber deposition, as well as the influence of spinning voltage and mesh size on the shape and precision of three-dimensional grid structure were investigated. The results showed that with the increase of setting distance of parallel fibers, the deposition error of fibers decreased and eventually stabilized. For the three-dimensional grid structure, as the voltage increases, the maximum number of deposited layers first increased and then decreased. When the spinning voltage was 6 kV, the maximum number of deposited layers was 15. The molding accuracy error first reduced and then increased. When the spinning voltage was 7 kV, the highest precision error was less than 5%. As the length of the grid increased, the number of layers continuously increased and the molding accuracy was gradually improved. When the side length of the mesh was greater than or equal to 1.5 mm, the deposition error tended to be stable and was maintained at about 5%.

Key words: direct writing electrospinning, polycaprolactone, morphology, molding precision, tissue engineering

中图分类号:

雷文龙, 张莉彦, 李轶, 杨卫民, 李好义, 陈明军. 电纺直写制备聚己内酯支架形貌及精度[J]. 化工进展, 2019, 38(05): 2300-2306.

Wenlong LEI, Liyan ZHANG, Yi LI, Weimin YANG, Haoyi LI, Mingjun CHEN. Morphology and accuracy of polycaprolactone (PCL) scaffolds prepared by electrospinning direct writing[J]. Chemical Industry and Engineering Progress, 2019, 38(05): 2300-2306.

图/表 8

图1 熔体电纺三维可控成型设备

图2 不同设定距离下电纺直写纤维沉积形貌

图3 设定间距对沉积误差的影响

表1 不同电压下可沉积的最大层数

进料速度 /μm·s^-1	接收距离 /mm	纺丝电压 /kV	接收板移动速度 /mm·min^-1	最大层数 /层
80	8	4	550	10
80	8	5	800	10
80	8	6	950	15
80	8	7	1500	12
80	8	8	2000	10

图4 不同电压下三维网格沉积10层垂直形貌图与倾斜形貌

图5 不同电压条件下网格参数误差

图6 不同设定间距下最大层数时网格垂直形貌与倾斜形貌

图7 不同设定间距下三维网格参数误差

参考文献 17

1	王隆太 . 先进制造技术[M]. 2版. 北京：机械工业出版社, 2017: 106.
	WANG Longtai . Advanced manufacturing technology.[M] 2nd Ed. Beijing：Mechanical Industry Press , 2017：106.
2	B-S KIM , MOONEY D J . Development of biocompatible synthetic extracellular matrices for tissue engineering[J]. Trends in Biotechnology, 1998, 16 (5): 224-230.
3	CHIRILA T V , HIGGINS B , DALTON P D . The effect of synthesis conditions on the properties of poly(2-hydroxyethyl methacrylate) sponges [J]. Cellular Polymers, 1998, 17(3): 141-162.
4	叶建东, 罗方聪 . 造孔粒子形貌对磷酸钙骨水泥支架材料结构与性能的影响[J]. 华南理工大学学报(自然科学版), 2010(4): 71-75.
	YE Jiandong , LUO Fangcong . Effect of porogen morphology on structure and property of calcium phosphate cement scaffold[J]. Journal of South China University of Technology(Natural Science Edition),2010(4): 71-75.
5	PANT H R , NAM K T , OH H J , et al . Effect of polymer molecular weight on the fiber morphology of electrospun mats[J]. Journal of Colloid and Interface Science, 2011, 364(1): 107-111.
6	KHODA A K , OZBOLAT I T , KOC B . A functionally gradient variational porosity architecture for hollowed scaffolds fabrication[J]. Biofabrication, 2011, 3(3): 034106.
7	高晓东, 杨卫民, 迟百宏,等 . 聚酰胺12制品3D打印成型力学性能研究[J]. 中国塑料, 2015 (12): 73-76.
	GAO Xiaodong , YANG Weimin , CHI Baihong , et al . Research on mechanical properties of PA12 products by 3D printing[J]. China Plastics, 2015(12): 73-76.
8	毛伟, 连芩, 李涤尘, 等 . 立体空心血管网水凝胶支架的3D打印工艺研究[J].机械工程学报, 2017, 53(9): 180-186.
	MAO Wei , LIAN Qin , LI Dichen , et al . 3D printing process for hydro gel with the three-dimensional micro tubes to mimic vascular network[J]. Journal of Mechanical Engineering, 2017, 53(9):180-186.
9	刘媛媛, 梁刚, 李瑜, 等 . 含微通道网络的再生支架3D打印成形工艺和系统及试验研究[J].机械工程学报,2015,51(21):137-147.
	LIU Yuanyuan, LIANG Gang, LI Yu, et al The 3D printing process and forming system for regenerated scaffold containing microchannel network and the experimental research[J]. Journal of Mechanical Engineering, 2015,51(21):137-147.
10	李星云, 李众立, 李理 . 熔融沉积成型工艺的精度分析与研究.[J] 制造技术与机床, 2014(9): 152-156.
	LI Xingyun , LI Zhongli , LI Li . Precision analysis and research of fused deposition molding[J]. Manufacturing Technology & Machine Tool, 2014(9): 152-156.
11	刘晓凤, 张莉彦, 谭晶, 等 . 熔体电纺聚乳酸可控结构成型工艺初探[J]. 工程塑料应用, 2016(5): 51-54.
	LIU Xiaofeng , ZHANG Liyan , TAN Jing , et al . Study on forming process of controlled structure about polylactic acid by melt electrospinning[J]. Engineering Plastics Application , 2016(5): 51-54.
12	RENEKER D H , YARIN A L , FONG H , et al . Bending instability of electrically charged liquid jets of polymer solutions in electrospinning[J]. Journal of Applied Physics ,2000 (87): 4531-4547.
13	赵扬, 姜佳昕, 张恺, 等 . 基于电纺直写的图案化微纳结构喷印技术[J]. 光学精密工程, 2016 (24): 2224-2231.
	ZHAO Yang , JIANG Jiaxin , ZHANG Kai , et al . Precision deposition of micro/nano pattern printed by electrohydrodynamic direct-write[J].Optics and Precision Engineering, 2016(24): 2224-2231.
14	BROWN T D , DALTON P D , HUTMACHER D W . Direct writing by way of melt electrospinning[J]. Advanced Materials, 2011(23): 5651-5657.
15	HAIGH J N , DARGAVILLE T R , DALTON P D . Additive manufacturing with polypropylene microfibers[J]. Materials Science & Engineering C: Materials for Biological Applications, 2017(77): 883-887.
16	JIN C , THYGESEN K S . Dynamical image charge effect in molecular tunnel junctions: beyond energy level alignment[J]. Physics, 2013, 89(4):1-8.
17	刘兆香, 刘勇, 王欣, 等 . 静电纺丝过程中泰勒锥、射流鞭动和电晕现象分析[J]. 塑料, 2012, 41(3): 29-34.
	LIU Zhaoxiang , LIU Yong , WANG Xin , et al . Phenomena analysis of taylor cone，jet whipping and corona in process of electrospinning[J]. Plastics, 2012, 41(3): 29-34.

[1]	刘毅, 房强, 钟达忠, 赵强, 李晋平. Ag/Cu耦合催化剂的Cu晶面调控用于电催化二氧化碳还原[J]. 化工进展, 2023, 42(8): 4136-4142.
[2]	张凯, 吕秋楠, 李刚, 李小森, 莫家媚. 南海海泥中甲烷水合物的形貌及赋存特性[J]. 化工进展, 2023, 42(7): 3865-3874.
[3]	陈怡欣, 甄摇摇, 陈瑞浩, 吴继伟, 潘丽美, 姚翀, 罗杰, 卢春山, 丰枫, 王清涛, 张群峰, 李小年. 铂基纳米催化剂的制备及在加氢领域的进展[J]. 化工进展, 2023, 42(6): 2904-2915.
[4]	张孟旭, 王红琴, 李金, 安霓虹, 戴云生, 钱颖, 沈亚峰. PtSn/MgAl₂O₄-sheet催化剂的制备及其PDH反应性能[J]. 化工进展, 2023, 42(3): 1365-1372.
[5]	田亚州, 胡钰婧, 李继友, 任江燕, 王立伟, 王修利, 丁颖, 程珏, 张军营. 香草醇基环氧树脂的合成、固化动力学及性能[J]. 化工进展, 2022, 41(S1): 477-484.
[6]	王慧, 刘新懿, 王伟, 万同, 厉宗洁, 王劭妤, 程博闻. 静电纺特殊形貌纳米纤维的应用研究进展[J]. 化工进展, 2022, 41(8): 4341-4356.
[7]	张嘉琪, 林丽娜, 高文桂, 祝星. CeO₂的形貌对CuO/CeO₂催化剂CO₂加氢制甲醇性能的影响[J]. 化工进展, 2022, 41(8): 4213-4223.
[8]	孙娜娜, 孙会娜, 沈莉莎, 苏瑞宇, 赵超. 磁性纳米颗粒-微波辐射对稠油O/W乳状液的协同破乳[J]. 化工进展, 2022, 41(6): 3127-3137.
[9]	马殿普, 李俊, 覃德清, 袁英杰, 潘飞, 符泽卫. 锡酸锌纳米材料的制备方法及应用研究进展[J]. 化工进展, 2022, 41(6): 3113-3126.
[10]	刘荣涛, 张诗洋, 黄兴文, 朋小康, 闵永刚. 聚苯胺/聚乳酸复合纳米纤维表面形貌对生物相容性的影响[J]. 化工进展, 2021, 40(8): 4406-4412.
[11]	冯江涛, 王睎, 赵旭阳, 龚向红, 延卫. 改性聚吡咯材料去除水中氟离子的性能[J]. 化工进展, 2021, 40(7): 4036-4046.
[12]	罗菊香, 程德书, 李明春, 辛梅华. 室温可见光引发聚合诱导自组装制备P2VP-b-PSt纳米材料[J]. 化工进展, 2021, 40(5): 2676-2684.
[13]	周雁红, 李夏兰, 张光亚. 生物大分子介导仿生矿化制备磁性纳米粒子的研究进展[J]. 化工进展, 2021, 40(5): 2719-2729.
[14]	陈少华, 陈文良, 丁益, 赵东林, 谢发之, 任启芳. 纳米材料及其三维结构修饰电极检测多巴胺的研究进展[J]. 化工进展, 2021, 40(11): 6135-6144.
[15]	余伟雄, 程高, 崔晓娥, 曾小红, 蓝邦, 李永峰, 张灿仰, 孙明, 余林. 不同形貌ɛ-MnO₂催化剂的制备及其催化燃烧甲苯性能[J]. 化工进展, 2021, 40(10): 5547-5553.