关键词: 嗜盐微生物, 下一代工业生物技术, 聚羟基脂肪酸酯, PHA, 合成生物学, 生物反应器, 聚合物

Abstract: Halophilic microorganisms including halophilic bacteria, archaea and algae, can be grown under high salt concentration conditions. Among them, the mild halophiles tolerating 30-150 g/L NaCl are valuable for application purposes. Many halophiles can grow rapidly in seawater under alkali conditions in minimal media, and they are strong candidates as chassis for industrial purposes. Recently, more and more molecular tools and approaches have been developed for manipulating halophiles, resulting in a longer product pipeline including biopolymers, proteins, chemicals, amino acids and cosmetic ingredients. The use of synthetic biology methods allows controllable morphology changes of halophiles for more intracellular product formation and downstream separation of the cell. Based on the recent progresses of halophiles, especially halophilic bacteria, we propose the halophilic bacteria based “Next generation industrial biotechnology” (NGIB), which allows microbial fermentations to be conducted under open unsterile and continuous processes using seawater as a medium to save fresh water and energy for sterilization. Halophilic bacteria are more robust than other traditional microbial chassis. They can be full-automatic controlled under continuous way to maintain consistent growth conditions. The unsterile processes significantly reduce the process complexity and equipment cost, further enhancing the product competitiveness. Co-production of intracellular and extracellular molecules increase the economic benefits of the NGIB.

Key words: Halophile, Next Generation Industrial Biotechnology（NGIB）, Polyhydroxyalkanoates, PHA, Synthetic biology, Bioreactors, Polymers