Synthesis and identification of bacterial cellulose

doi:10.16085/j.issn.1000-6613.2020-0384

Abstract

Abstract:

Bacterial cellulose (BC) has been widely used in medicine, food and other fields because of its unique properties. Based on the domestic and foreign literature and team research results, this paper reviews the related research on BC synthesis and identification. Firstly, the screening of BC synthetic bacteria and the utilization of carbon source were analyzed, and the research ideas of reducing BC synthesis cost were summarized. Secondly, the methods to identify the synthetic products of strains were summarized, and the characteristics of different methods were summarized. Then combined with the team selected BC production bacteria XJL-06-4BC synthesis The results of enzyme gene analysis reviewed the BC synthesis pathway, the form of synthetase and the regulation of gene level, a new idea for BC to increase yield by changing the synthesis pathway at the molecular level. Finally, summarize the problems existing in BC microbial fermentation production, and put forward solutions from many angles.

Key words: bacterial cellulose, carbon source utilization, product identification, synthetic pathway, synthase

摘要：

细菌纤维素（BC）因其独特性能被广泛应用于医药、食品等领域，目前其高产量菌株筛选、合成成本降低及合成途径改良等成为研究热点。本文依据国内外文献并结合团队研究成果对BC合成与鉴定的相关研究进行综述。首先对BC合成菌筛选及碳源利用的研究进行了分析，总结了降低BC合成成本的研究思路。其次对鉴定菌株合成产物的方法进行了归纳，总结了不同方法的特点。然后结合本团队筛选出的BC生产菌XJL-06-4 BC合成酶基因分析结果，综述了BC合成途径、合成酶存在形式以及基因水平调控作用，为BC在分子水平上通过改变合成途径提高产量提供新思路。最后，总结BC微生物发酵生产存在的问题，多角度提出解决方案。

关键词: 细菌纤维素, 碳源利用, 产物鉴定, 合成途径, 合成酶

CLC Number:

Xin ZHAO, Jianli XIONG, Yelin REN, Jiaxin YANG, Wei LI, Xuerong HAN. Synthesis and identification of bacterial cellulose[J]. Chemical Industry and Engineering Progress, 2020, 39(S2): 262-268.

赵鑫, 熊健力, 任叶琳, 杨家鑫, 李伟, 韩雪容. 细菌纤维素合成与鉴定研究综述[J]. 化工进展, 2020, 39(S2): 262-268.

Figures/Tables 5

References 42

1	BROWN A J. On an acetic ferment which forms cellulose[J]. Chem. Soc., 1886, 49: 432-439.
2	付莉. 细菌纤维素的研究综述[J].食品研究与开发, 2004, 25(2): 18-20.
	FU Li. A review of bacterial cellulose[J]. Food Research and Development, 2004, 25(2): 18-20.
3	胡佳琪, 汪思维, 曾志涛, 等.细菌纤维素在食品和面膜中的应用研究进展[J].科技与创新, 2016(6): 2-3.
	HU Jiaqi, WANG Siwei, ZENG Zhitao, et al. Application of bacterial cellulose in food and mask[J]. Technology and Innovation, 2016(6): 2-3.
4	陈竞, 冯蕾, 杨新平, 等. 细菌纤维素的制备和应用研究进展[J]. 纤维素科学与技术, 2014, 22(2): 58-63.
	CHEN Jing, FENG Lei, YANG Xinping, et al.Research progress of preparation and application of bacterial cellulose[J]. Cellulose Science and Technology, 2014, 22(2): 58-63.
5	周毓, 刘艳. 细菌纤维素研究进展[J].广州化工, 2007, 35(2): 8-9.
	ZHOU Yu, LIU Yan. Research progress of bacterial cellulose[J]. Guangzhou Chemical Industry, 2007, 35(2): 8-9.
6	马承铸,顾真荣.细菌纤维素生物理化性能和商业用途[J]. 上海农业学报, 2001, 17(4): 93-98.
	MA Chengzhu, GU Zhenrong. Biophysicochemical properties and commercial use of bacterial cellulose[J]. Shanghai Journal of Agriculture, 2001, 17(4): 93-98.
7	YAMANAKA S, SUGIYAMA J. Structural modification of bacterial cellulose[J]. Cellulose, 2000, 7(3): 213-225.
8	BI J C, LIU S X, LI C F, et al. Morphology and structure characterization of bacterial celluloses produced by different strains in agitated culture[J]. Journal of Applied Microbiology, 2014, 117(5): 1305-1311.
9	FUJIWARA T, KOMODA K, SAKURAI N, et al. The c-di-GMP recognition mechanism of the PilZ domain of bacterial cellulose synthase subunit A[J]. Biochemical and Biophysical Research Communications, 2013, 431(4): 802-807.
10	SERRA D O, RICHTER A M, HENGGE R. Cellulose as an architectural element in spatially structured Escherichia coli biofilms[J]. Journal of Bacteriology, 2013, 195(24): 5540-5554.
11	WHITE A P, WELJIE A M, APEL D, et al. A global metabolic shift is linked to Salmonella multicellular development[J]. PLoS One, 2010, 5: e11814.
12	施庆珊, 冯劲, 冯静,等. 一株产细菌纤维素菌株的分离和初步鉴定[J]. 发酵科技通讯, 2009, 38(2): 11-14.
	SHI Qingshan, FENG Jin, FENG Jing, et al. Isolation and preliminary identification of a bacterial cellulose strain[J]. Fermentation Technology Newsletter, 2009, 38(2): 11-14.
13	周胜虎, 薛齐佳, 刘传凤, 等. 细菌纤维素高产菌株的筛选和初步鉴定[J]. 湖北农业科学, 2013, 52(15): 3514-3517.
	ZHOU Shenghu, XUE Qijia, LIU Chuanfeng, et al. Screening and preliminary identification of high yield strains of bacterial cellulose[J]. Hubei Agricultural Science, 2013, 52(15): 3514-3517.
14	朱宏阳, 姚俊, 冯珊, 等.1株产细菌纤维素芽胞杆菌的分离及鉴定[J]. 生物加工过程, 2014, 12(5): 80-84.
	ZHU Hongyang, YAO Jun, FENG Shan, et al. Isolation and identification of bacterial bacillus cellulose[J]. Bioprocessing Process, 2014, 12(5): 80-84.
15	REVIN V, LIYASKINA E, NAZARKINA M, et al. Cost-effective production of bacterial cellulose using acidic food industry by-products[J]. Brazilian Journal of Microbiology, 2018, 49(1): 151-159.
16	WU J M, LIU R H. Cost-effective production of bacterial cellulose in static cultures using distillery wastewater[J]. Biosci. Bioeng., 2013, 115(3): 284-290.
17	JAIN S, GUPTA R. Development of low cost nutritional beverage from whey[J]. Environ. Sci .Toxicol. Food Technol., 2013(5): 73-88.
18	CARREIRA P, MENDES J A S, TROVATTI E, et al. Utilization of residues from agro-forest industries in the production of high value bacterial cellulose[J]. Bioresour. Technol., 2011, 102:7354-7360.
19	KOSE R, SUNAGAWA N, YOSHIDA M, et al. One-step production of nanofibrillated bacterial cellulose (NFBC) from waste glycerol using Gluconacetobacter intermedius NEDO-01[J]. Cellulose, 2013(20): 2971-2979.
20	袁金霞, 王婷, 黄显南, 等.细菌纤维素在造纸工业中的应用研究进展[J]. 纸和造纸, 2016, 35(7): 42-46.
	YUAN Jinxia, WANG Ting, HUANG Xiannan, et al. Application of bacterial cellulose in paper industry[J]. Paper and Paper, 2016, 35(7): 42-46.
21	李昊燃.红茶菌中细菌纤维素产生菌的筛选鉴定及发酵条件优化[D]. 开封: 河南大学, 2016.
	LI Haoran. Screening and identification of bacterial cellulose producing bacteria in black tea and optimization of fermentation conditions[D]. Kaifeng: Henan University, 2016.
22	李国辉.细菌纤维素纤维复合材料的制备及其应用研究[D]. 无锡: 江南大学, 2017.
	LI Guohui. Preparation and application of bacterial cellulose fiber composite materials[D]. Wuxi: Jiangnan University, 2017.
23	RÖMLING U, GALPERIN M Y. Bacterial cellulose biosynthesis: diversity of operons, subunits, products and functions[J]. HHS Public Access, 2015 23(9): 545-557.
24	DENG Y, NIVEDITA N, XIAO C W, et al. Identification and characterization of non-cellulose-producing mutants of Gluconacetobacter hansenii generated by Tn5 transposon mutagenesis[J]. Bacteriology, 2013, 195(22): 5072-5083.
25	NAOKI S, TAKAAKI F, TAKANORI Y, et al. Cellulose complementing factor (Ccp) is a new member of the cellulose synthase complex (terminal complex) in Acetobacter xylinum[J]. Biosci. Bioeng., 2013, 115: 607-612.
26	NAKAI T, SUGANO Y, SHODA M, et al. Formation of highly twisted ribbons in a carboxymethyl cellulase gene-disrupted strain of a cellulose-producing bacterium[J]. Bacteriology, 2013, 195: 958-964.
27	UMEDA Y, HIRANO A, ISHIBASHI M, et al. Cloning of cellulose synthase genes from Acetobacter xylinum JCM 7664: implication of a novel set of cellulose synthase genes[J]. DNA Res., 1999, 6: 109-115.
28	RÖMLING U. Molecular biology of cellulose production in bacteria[J]. Res. Microbiol., 2002, 153: 205-212.
29	ZOGAJ X, NIMTZ M, ROHDE M, et al. The multicellular morphotypes of salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix[J]. Mol. Microbiol., 2001, 39: 1452-1463.
30	QUERE B L, GHIGO J M. BcsQ is an essential component of the Escherichia coli cellulose biosynthesis apparatus that localizes at the bacterial cell pole[J]. Mol. Microbiol., 2009, 72: 724-740.
31	SOLANO C, GARCIA B, VALLE J, et al. Genetic analysis of Salmonella enteritidis biofilm formation: critical role of cellulose[J]. Mol. Microbiol., 2002, 43(3): 793-808.
32	SERRA D O, RICHTER A M, HENGGE R. Cellulose as an architectural element in spatially structured Escherichia coli biofilms[J]. Bacteriology, 2013, 195(24): 5540-5554.
33	NORA A, MARTIN L, STEFAN H, et al. Structural and putative regulatory genes involved in cellulose synthesis in Rhizobium leguminosarum bv.trifolii[J]. Microbiology, 1999, 145(5): 1253-1262.
34	LIGHTFOOT R, MATTHYSSE A G, WHITE S, et al. Genes required for cellulose synthesis in Agrobacterium tumefaciens[J]. Bacteriology, 1995, 177(4): 1069-1075.
35	KRALL L, FUQUA C, MARRY M, et al. The effect of cellulose overproduction on binding and biofilm formation on roots by Agrobacterium tumefaciens[J]. Mol. Plant Microbe. Interact., 2005, 18: 1002-1010.
36	HU S Q, GAO Y G, TAJIMA K, et al. Structure of bacterial cellulose synthase subunit D octamer with four inner passageways[J]. Proc. Natl. Acad. Sci. USA, 2010, 107(42): 17957-17961.
37	MEHTA K, PFEFFER S, BROWN R M, et al. Characterization of an acsD disruption mutant provides additional evidence for the hierarchical cell-directed self-assembly of cellulose in Gluconacetobacter xylinus[J]. Cellulose, 2015, 22(1): 119-137.
38	GARCIA B, LATASA C, SOLANO C, et al. Role of the GGDEF protein family in salmonella cellulose biosynthesis and biofilm formation[J]. Mol. Microbiol., 2004, 54(1): 264-277.
39	RÖMLING U. Characterization of the rdar morphotype, a multicellular behaviour in Enterobacteriaceae[J]. Cell Mol. Life Sci., 2005(62): 1234-1246.
40	MAYER R, TAL R, SAPIR S, et al. Three cdg operons control cellular turnover of cyclic di-GMP in Acetobacter xylinum: genetic organization and occurrence of conserved domains in isoenzymes[J]. Bacteriology, 1998, 180(17): 4416-4425.
41	RÖMLING U, ROHDE M, OLSEN A, et al. The checkpoint of multicellular and aggregative behaviour in salmonella typhimurium regulates at least two independent pathways[J]. Mol. Microbiol., 2000, 36(1): 10-23.
42	REDA S, GHIGO J M. A CsgD-independent pathway for cellulose production and biofilm formation in Escherichia coli[J]. Bacteriology, 2006, 188(8): 3073-3087.

名称	起始位点	结束位点	主要作用
bcsQ	2321	3403	细胞定位、细胞间黏附
bcsZ	19944	21065	调控纤维素的合成

名称	起始位点	结束位点	主要作用
bcsQ	2321	3403	细胞定位、细胞间黏附
bcsZ	19944	21065	调控纤维素的合成

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