Chemical Industry and Engineering Progress ›› 2022, Vol. 41 ›› Issue (10): 5653-5660.DOI: 10.16085/j.issn.1000-6613.2021-2610
• Resources and environmental engineering • Previous Articles Next Articles
WANG Runmin1(), ZHANG Xiaodong1, XU Chenghua2, YU Dandan2, YU Ran1()
Received:
2021-12-22
Revised:
2022-03-10
Online:
2022-10-21
Published:
2022-10-20
Contact:
YU Ran
汪润民1(), 张晓东1, 徐成华2, 于丹丹2, 余冉1()
通讯作者:
余冉
作者简介:
汪润民(1997—),男,硕士研究生,研究方向为生物技术在污染场地环境生态修复中的应用。E-mail:seuwrm0815@foxmail.com。
基金资助:
CLC Number:
WANG Runmin, ZHANG Xiaodong, XU Chenghua, YU Dandan, YU Ran. Construction and performance evaluation of high efficiency heavy oil degradation consortium[J]. Chemical Industry and Engineering Progress, 2022, 41(10): 5653-5660.
汪润民, 张晓东, 徐成华, 于丹丹, 余冉. 高效重质石油降解菌群构建及降解性能评价[J]. 化工进展, 2022, 41(10): 5653-5660.
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URL: https://hgjz.cip.com.cn/EN/10.16085/j.issn.1000-6613.2021-2610
降解时间/d | 指数 | |||||
---|---|---|---|---|---|---|
shannon | simpson | ace | chao | shannoneven | simpsoneven | |
10 | 2.995±0.188 | 0.103±0.024 | 172.507±7.508 | 171.393±6.669 | 0.586±0.039 | 0.062±0.018 |
30 | 3.133±0.076 | 0.078±0.011 | 187.215±7.297 | 186.446±5.562 | 0.603±0.017 | 0.073±0.010 |
50 | 3.006±0.060 | 0.102±0.008 | 195.740±6.982 | 194.525±3.151 | 0.575±0.008 | 0.053±0.003 |
降解时间/d | 指数 | |||||
---|---|---|---|---|---|---|
shannon | simpson | ace | chao | shannoneven | simpsoneven | |
10 | 2.995±0.188 | 0.103±0.024 | 172.507±7.508 | 171.393±6.669 | 0.586±0.039 | 0.062±0.018 |
30 | 3.133±0.076 | 0.078±0.011 | 187.215±7.297 | 186.446±5.562 | 0.603±0.017 | 0.073±0.010 |
50 | 3.006±0.060 | 0.102±0.008 | 195.740±6.982 | 194.525±3.151 | 0.575±0.008 | 0.053±0.003 |
降解时间/d | 优势菌属 | 丰度/% |
---|---|---|
10 | Pseudomonas | 22.6±8.9 |
Ottowia | 15.7±3.6 | |
Reyranella | 11.2±4.7 | |
Moheibacter | 6.0±4.7 | |
Parvibaculum | 5.2±0.5 | |
30 | Pseudomonas | 15.6±5.4 |
Moheibacter | 14.3±3.5 | |
Reyranella | 9.8±0.9 | |
Immundisolibacter | 9.3±1.1 | |
Parvibaculum | 7.2±1.7 | |
Pseudoxanthomonas | 7.1±2.8 | |
50 | Parvibaculum | 26.1±1.3 |
Pseudomonas | 12.8±0.4 | |
Reyranella | 12.1±0.5 | |
Pseudoxanthomonas | 10.0±1.0 |
降解时间/d | 优势菌属 | 丰度/% |
---|---|---|
10 | Pseudomonas | 22.6±8.9 |
Ottowia | 15.7±3.6 | |
Reyranella | 11.2±4.7 | |
Moheibacter | 6.0±4.7 | |
Parvibaculum | 5.2±0.5 | |
30 | Pseudomonas | 15.6±5.4 |
Moheibacter | 14.3±3.5 | |
Reyranella | 9.8±0.9 | |
Immundisolibacter | 9.3±1.1 | |
Parvibaculum | 7.2±1.7 | |
Pseudoxanthomonas | 7.1±2.8 | |
50 | Parvibaculum | 26.1±1.3 |
Pseudomonas | 12.8±0.4 | |
Reyranella | 12.1±0.5 | |
Pseudoxanthomonas | 10.0±1.0 |
组分 | 降解菌群 | Pearson 系数 | Spearman系数 | Kendall 系数 | 50d降解率 |
---|---|---|---|---|---|
饱和烃 | QD强化后 | -0.824* | -0.817* | -0.611* | 46.93%±0.55% |
QM | -0.916* | -0.950* | -0.889* | 73.71%±7.56% | |
芳香烃 | QD强化后 | -0.723* | -0.517 | -0.444 | 40.83%±5.50% |
QM | -0.805* | -0.933* | -0.833* | 53.17%±5.41% | |
胶质 | QD强化后 | -0.769* | -0.767* | -0.611* | 36.15%±7.09% |
QM | 0.296 | 0.300 | 0.167 | 12.61%±0.33% | |
沥青质 | QD强化后 | -0.213 | -0.200 | -0.167 | 10.58%±12.40% |
QM | -0.748* | -0.817* | -0.667* | 25.56%±5.40% |
组分 | 降解菌群 | Pearson 系数 | Spearman系数 | Kendall 系数 | 50d降解率 |
---|---|---|---|---|---|
饱和烃 | QD强化后 | -0.824* | -0.817* | -0.611* | 46.93%±0.55% |
QM | -0.916* | -0.950* | -0.889* | 73.71%±7.56% | |
芳香烃 | QD强化后 | -0.723* | -0.517 | -0.444 | 40.83%±5.50% |
QM | -0.805* | -0.933* | -0.833* | 53.17%±5.41% | |
胶质 | QD强化后 | -0.769* | -0.767* | -0.611* | 36.15%±7.09% |
QM | 0.296 | 0.300 | 0.167 | 12.61%±0.33% | |
沥青质 | QD强化后 | -0.213 | -0.200 | -0.167 | 10.58%±12.40% |
QM | -0.748* | -0.817* | -0.667* | 25.56%±5.40% |
1 | AJONA M, VASANTHI P. Bioremediation of petroleum contaminated soils — A review[J]. Materials Today: Proceedings, 2021, 45: 7117-7122. |
2 | DEVATHA C P, VISHNU VISHAL A, PURNA CHANDRA RAO J. Investigation of physical and chemical characteristics on soil due to crude oil contamination and its remediation[J]. Applied Water Science, 2019, 9(4): 1-10. |
3 | 李照, 许玉玉, 张世凯, 等. 海洋溢油污染及修复技术研究进展[J]. 山东建筑大学学报, 2020, 35(6): 69-75. |
LI Zhao, XU Yuyu, ZHANG Shikai, et al. Research progress of marine petroleum pollution and remediation technology[J]. Journal of Shandong Jianzhu University, 2020, 35(6): 69-75. | |
4 | 丁克强, 孙铁珩, 李培军. 石油污染土壤的生物修复技术[J]. 生态学杂志, 2000, 19(2): 50-55. |
DING Keqiang, SUN Tieheng, LI Peijun. Bioremediation of the soil contaminated by petroleum hydrocarbons[J]. Chinese Journal of Ecology, 2000, 19(2): 50-55. | |
5 | 王丽静, 尚振坤, 张兴昌, 等. 原油污染对黄绵土和风沙土水分入渗的影响[J]. 应用生态学报, 2021, 32(9): 3341-3348. |
WANG Lijing, SHANG Zhenkun, ZHANG Xingchang, et al. Effects of crude oil pollution on soil moisture infiltration with loessial soil and aeolian sandy soil[J]. Chinese Journal of Applied Ecology, 2021, 32(9): 3341-3348. | |
6 | LI Qian, YOU Ping, HU Qi, et al. Effects of co-contamination of heavy metals and total petroleum hydrocarbons on soil bacterial community and function network reconstitution[J]. Ecotoxicology and Environmental Safety, 2020, 204: 111083. |
7 | O’BRIEN Peter L, DESUTTER Thomas M, CASEY Francis X M, et al. Evaluation of soil function following remediation of petroleum hydrocarbons—A review of current remediation techniques[J]. Current Pollution Reports, 2017, 3(3): 192-205. |
8 | 钟磊, 卿晋武, 陈红云, 等. 微生物修复石油烃土壤污染技术研究进展[J]. 生物工程学报, 2021, 37(10): 3636-3652. |
ZHONG Lei, QING Jinwu, CHEN Hongyun, et al. Advances in bioremediation of hydrocarbon-contaminated soil[J]. Chinese Journal of Biotechnology, 2021, 37(10): 3636-3652. | |
9 | Lucia ROMERO-HERNÁNDEZ, VELEZ Patricia, Itandehui BETANZO-GUTIÉRREZ, et al. Extra-heavy crude oil degradation by Alternaria sp. isolated from deep-sea sediments of the gulf of Mexico[J]. Applied Sciences, 2021, 11(13): 6090. |
10 | 张小梅, 孔萌, 邢献杰, 等. 双效工程菌BBb对炼化油泥中总石油烃的降解特性[J]. 环境化学, 2021, 40(7): 2255-2264. |
ZHANG Xiaomei, KONG Meng, XING Xianjie, et al. Degradation characteristics of total petroleum hydrocarbons in refining sludge by dual-function bacteria BBb[J]. Environmental Chemistry, 2021, 40(7): 2255-2264. | |
11 | YANG Yong, ZHANG Zhanwei, LIU Ruixia, et al. Research progress in bioremediation of petroleum pollution[J]. Environmental Science and Pollution Research, 2021, 28(34): 46877-46893. |
12 | LIU Wendan. Advances in research on rehabilitation of plants on petroleum pollution soil[J]. World Scientific Research Journal, 2021, 7(6): 27-32. |
13 | 李小康, 鱼涛, 李红, 等. 石油降解菌的复配及降解效果评价[J]. 油田化学, 2020, 37(2): 311-317, 324. |
LI Xiaokang, YU Tao, LI Hong, et al. Analysis of compounding and degradation of petroleum degrading bacteria[J]. Oilfield Chemistry, 2020, 37(2): 311-317, 324. | |
14 | DUC H D, HUNG N V, OANH N T. Anaerobic degradation of endosulfans by a mixed culture of Pseudomonas sp. and Staphylococcus sp[J]. Applied Biochemistry and Microbiology, 2021, 57(3): 327-334. |
15 | ZHAO Yuxiang, WANG Jiaqi, LIU Yan, et al. Microbial interaction promotes desulfurization efficiency under high pH condition[J]. Environmental Research, 2021, 200: 111423. |
16 | LI Qiqian, LI Jibing, JIANG Longfei, et al. Diversity and structure of phenanthrene degrading bacterial communities associated with fungal bioremediation in petroleum contaminated soil[J]. Journal of Hazardous Materials, 2021, 403: 123895. |
17 | 左丽敏, 马晓阳, 李智民, 等. 土壤中石油烃与降解菌群的相互作用研究[J]. 安全与环境工程, 2015, 22(4): 63-68. |
ZUO Limin, MA Xiaoyang, LI Zhimin, et al. Research on interaction between petroleum hydrocarbon and degrading bacteria consortium in soil[J]. Safety and Environmental Engineering, 2015, 22(4): 63-68. | |
18 | 国家能源局. 岩石中可溶有机物及原油族组分分析: [S]. 北京:中国标准出版社,2016. |
National Energy Bureau of the People's Republic of China. Analytical method of soluble organic matters in rocks and crude oil group composition column chromatography: [S]. Beijing: China Standard Press, 2016. | |
19 | CHEN S, ZHOU Y, CHEN Y, et al. Fastp: an ultra-fast all-in-one FASTQ preprocessor [J]. Bioinformatics, 2018, 34(17): i884-i890. |
20 | Tanja MAGOČ, SALZBERG Steven L. FLASH: fast length adjustment of short reads to improve genome assemblies[J]. Bioinformatics (Oxford, England), 2011, 27(21): 2957-2963. |
21 | EDGAR Robert C. UPARSE: highly accurate OTU sequences from microbial amplicon reads[J]. Nature Methods, 2013, 10(10): 996-998. |
22 | STACKEBRANDT E, GOEBEL B M. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology[J]. International Journal of Systematic and Evolutionary Microbiology, 1994, 44(4): 846-849. |
23 | WANG Qiong, GARRITY George M, TIEDJE James M, et al. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J]. Applied and Environmental Microbiology, 2007, 73(16): 5261-5267. |
24 | VARJANI Sunita, UPASANI Vivek N. Bioaugmentation of Pseudomonas aeruginosa NCIM 5514—A novel oily waste degrader for treatment of petroleum hydrocarbons[J]. Bioresource Technology, 2021, 319: 124240. |
25 | EBADI Ali, SIMA Nayer Azam Khoshkholgh, OLAMAEE Mohsen, et al. Effective bioremediation of a petroleum-polluted saline soil by a surfactant-producing Pseudomonas aeruginosa consortium[J]. Journal of Advanced Research, 2017, 8(6): 627-633. |
26 | CHETTRI Bobby, SINGHA Ningombam A, SINGH Arvind Kumar. Efficiency and kinetics of Assam crude oil degradation by Pseudomonas aeruginosa and Bacillus sp[J]. Archives of Microbiology, 2021, 203(9): 5793-5803. |
27 | SONG Wenfeng, WANG Jianwei, YAN Yingchun, et al. Shifts of the indigenous microbial communities from reservoir production water in crude oil- and asphaltene-degrading microcosms[J]. International Biodeterioration & Biodegradation, 2018, 132: 18-29. |
28 | CHEN Qingguo, BAO Bo, LI Yijing, et al. Effects of marine oil pollution on microbial diversity in coastal waters and stimulating indigenous microorganism bioremediation with nutrients[J]. Regional Studies in Marine Science, 2020, 39: 101395. |
29 | PATEL Vilas, CHETURVEDULA Sravanthi, MADAMWAR Datta. Phenanthrene degradation by Pseudoxanthomonas sp. DMVP2 isolated from hydrocarbon contaminated sediment of Amlakhadi canal, Gujarat, India[J]. Journal of Hazardous Materials, 2012, 201/202: 43-51. |
30 | NAYAK Anand S, VIJAYKUMAR M H, KAREGOUDAR T B. Characterization of biosurfactant produced by Pseudoxanthomonas sp. PNK-04 and its application in bioremediation[J]. International Biodeterioration & Biodegradation, 2009, 63(1): 73-79. |
31 | POURFAKHRAEI Elaheh, BADRAGHI Jalil, MAMASHLI Fatemeh, et al. Biodegradation of asphaltene and petroleum compounds by a highly potent Daedaleopsis sp.[J]. Journal of Basic Microbiology, 2018, 58(7): 609-622. |
32 | 尹凌皓, 辛瑞, 郝博宇, 等. 高效稠油降解降黏菌群的构建及其性能评价[J]. 石油学报(石油加工), 2021, 37(5): 1174-1181. |
YIN Linghao, XIN Rui, HAO Boyu, et al. Construction and performance evaluation of high efficiency heavy oil degradation and viscosity reduction consortium[J]. Acta Petrolei Sinica (Petroleum Processing Section), 2021, 37(5): 1174-1181. |
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