Evaluation and analysis of activity assays of ligninolytic enzymes

doi:10.16085/j.issn.1000-6613.2020-0248

Abstract

Abstract:

As one of the most abundant reproducible organic aromatic polymers, bioconversion and valorization of lignin have considerable environmental, economic and social benefits. However, the lack of consolidation of the enzymatic assays of the ligninolytic enzymes has brought difficulties for the research on bioconversion of lignin. In this paper, we have compared and evaluated the sources, properties, and enzyme-substrate interaction mechanisms towards a few key ligninolytic enzymes including laccase (Lac), manganese peroxidase (MnP), lignin peroxidase (LiP), versatile peroxidase (VP) and dye-decoloration peroxidase (DyP). By discussions from the theory to applications of the most common assays including the ABTS method, 2,6-DMP method, VA method, Azure B method and other methods, the variety of substrates, assay conditions and scope of applications were thoroughly evaluated. In addition, solutions were provided for the major challenges existed in the enzymatic assays of ligninolytic enzymes, and this will offer valuable strategy for the bio-degradation of lignin and its value-added applications.

Key words: lignin, biodegradation, polymers, ligninolytic enzymes, enzymatic assays

摘要：

作为最丰富的可再生有机芳香族聚合物之一，木质素的生物降解和利用具有较好的环境、经济和社会效益，但繁多的木质素降解酶活测试方法为生物降解木质素的相关研究带来一定困难。本文围绕已发现的5种重要木质素降解酶（漆酶、锰过氧化物酶、木质素过氧化物酶、多功能过氧化物酶和染料脱色过氧化物酶），针对其来源、特性和酶与底物的作用机理，深入地比较和分析了多种木质素降解酶酶活测试方法。通过对ABTS法、 2,6-DMP法、藜芦醇法、天青B法和其他特色方法从原理到应用的综合讨论，全面评估了上述方法的底物多样性、测试条件及适用范围。此外，本文还针对木质素降解酶酶活测试研究中所存在的主要挑战提出了解决思路，旨在为生物法降解木质素及其高值化利用的研究提供有效参考和策略。

关键词: 木质素, 生物降解, 聚合物, 木质素降解酶, 酶活测试

CLC Number:

Tangwu CUI, Bo YUAN, Chen LING, Binren FANG, Xiangyang MAO, Qiang FEI. Evaluation and analysis of activity assays of ligninolytic enzymes[J]. Chemical Industry and Engineering Progress, 2020, 39(12): 5189-5202.

崔堂武, 袁波, 凌晨, 方彬任, 毛向阳, 费强. 木质素降解酶的酶活测试方法的评价与分析[J]. 化工进展, 2020, 39(12): 5189-5202.

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