Preparation and application of mixed amino acids from waste penicillin mycelium by microwave hydrolysis

doi:10.16085/j.issn.1000-6613.2017.06.044

Abstract

Abstract: Waste penicillin mycelium(WPM) is solid waste from the fermentation process of penicillin in pharmaceutical industry,which will result in air pollution and threaten the ecological environment. WPM was treated by microwave hydrolysis in alkali condition to produce mixed amino acids,which can be used for gypsum retarder to realize safe utilization of WPM. Based on the single factor experiment,four factors were selected for orthogonal experiment:alkali concentration,solid-liquid ratio,treatment time,and temperature. The optimal conditions for the hydrolysis process were obtained:alkali concentration 0.04mol/L,solid-liquid ratio 1:4,reaction time 10min,and at 85℃. Chemical composition of the product was analyzed using high speed amino acid analyzer. The retarding mechanism for desulphurization gypsum was investigated using FTIR and SEM. The results showed that the retarder contained amino acids such as lysine,tyrosine,and cysteine,etc. The amino and carboxyl group in the retarder chelated with calcium ion,forming mixed amino acids/small peptides complexes,which delayed dissolution,hydration and crystallization of gypsum crystals,thereby prolonging the setting time of gypsum paste.

Key words: mycelium residue, gypsum retarder, microwave, protein, hydrolysis, mixed amino acids, petptide

摘要： 青霉素废菌渣是制药工业青霉素发酵工序产生的固体废弃物，处理不当会导致环境污染、危及生态。本文以废菌渣为原料，在碱性条件下，采用微波水解方法得到复合氨基酸。将其作为石膏缓凝剂，可以实现废菌渣的安全、高附加值利用。在单因素实验的基础上，以碱浓度、料液比、反应时间、反应温度为考察因素，采用正交实验法确定最佳工艺条件为：碱浓度0.04mol/L，料液比1∶4，微波水解时间10min，温度85℃。借助氨基酸分析仪分析了水解产物的化学组成，利用红外光谱、扫描电镜等手段对缓凝作用（石膏体系）机理进行了分析研究。结果表明：水解物主要是赖氨酸、酪氨酸、半胱氨酸等氨基酸的混合物，其中氨基和羧基官能团参与了钙的配位，形成了钙的复合氨基酸/小肽螯合物，从而延缓了石膏的溶解、水化和结晶过程，使凝结时间延长。

关键词: 废菌渣, 石膏缓凝剂, 微波, 蛋白质, 水解, 复合氨基酸, 肽

CLC Number:

X705

ZHANG Mengmeng, ZHAO Fengqing. Preparation and application of mixed amino acids from waste penicillin mycelium by microwave hydrolysis[J]. Chemical Industry and Engineering Progress, 2017, 36(06): 2275-2281.

张蒙蒙, 赵风清. 青霉素废菌渣微波水解制备复合氨基酸及应用[J]. 化工进展, 2017, 36(06): 2275-2281.

References

[1] 王丙,刘辉玲,王璞.青霉素菌渣理化特性及其资源化利用研究现状[J].环境工程,2014,32(2):139-142. WANG Bing,LIU Huiling,WANG Pu.Characteristics of penicillin mycelium and its state of resource utilization[J].Environmental Engineering,2014,32(2):139-142.
[2] 陈立文,方森海,王明兹.抗生素发酵废菌渣的无害化及资源再利用研究进展[J].生物技术通报,2015,31(5):13-19. CHEN Liwen,FANG Senhai,WANG Mingzi.Research progress on the harmless treatment and resource reuse of antibiotic bacteria residues[J].Biotechnology Bulletin,2015,31(5):13-19.
[3] MENG X L,MIAO Y,ZHU Y,et al.Study on the antibiotic bacterial residue for the human health risk assessment[J].Advanced Material Research,2013,788:476-479.
[4] 苏建文,王俊超,许尚营,等.红霉素菌渣厌氧消化实验研究[J].中国沼气,2013,31(5):25-28. SU Jianwen,WANG Junchao,XU Shangying,et al.Anaerobic digestion of bacterial residues from erythromycin production[J]. China Biogas,2013,31(5):25-28.
[5] 刘波文.青霉素菌丝体理化特性和化学法制备活性炭的研究[D].石家庄:河北科技大学,2010. LIU Bowen.Study on the characteristics of penicillin mycelium and the preparation of activated carbon with chemical method[D]. Shijiazhuang:Hebei University of Science & Technology.2010.
[6] 周保华,高勤,郭斌,等.响应面法优化青霉素菌渣活性炭制备技术的研究[J].河北科技大学学报,2012,33(6):554-558. ZHOU Baohua,GAO Qin,GUO Bin,et al.Optimization of activated carbon preparation from penicillin bacterial residue by response surface methodology[J].Journal of Hebei University of Science and Technology,2012,33(6):554-558.
[7] 韩庆,苏海佳.废菌渣高值化研究中细胞破壁工艺的比较[J].环境科学与技术,2011,34(5):144-147. HAN Qing,SU Haijia.Comparison of cell wall broken in the research of high value utilization of mycelium residue[J]. Environmental Science &Technonlogy,2011,34(5):144-147.
[8] 韩洪军,牟晋铭,马文成,等.微波辐射对青霉素菌渣破壁效果的影响[J].化工学报,2013,64(10):3812-3817. HAN Hongjun,MOU Jinming,MA Wencheng,et al.Effect of microwave on cell wall broken of penicillin fermentation residue[J].CIESE Journal,2013,64(10):3812-3817.
[9] 许家喜.微波与有机化学反应的选择性[J].化学进展,2007,19(5):700-712. XU Jiaxi. Microwave irradiation and selectivities in organic reactions[J].Progress in Chemistry,2007,19(5):700-712.
[10] 王传芬.从废菌丝体中提取壳聚糖的研究[J].中国酿造,2009(10):123-126. WANG Chuanfen.Extraction of chitosan from waste mycelium[J]. China Brewing,2009(10):123-126.
[11] 刘玲,吴正奇,王雄,等.青霉素菌丝体中麦角固醇分离和纯化研究[J].湖北大学学报(自然科学),2013(2):232-235. LIU Ling,WU Zhengqi,WANG Xiong,et al.Study on extraction of ergosterol from penicillin mycelium[J].Journal of Hubei University(Natural Science),2013(2):232-235.
[12] 韩庆雪,闻建平,冯国龙.麦角固醇工业化提取工艺研究[J].河北化工,2007,30(2):42-43. HAN Qingxue,WEN Jianping,FENG Guolong.Study on industrialized extraction processes of ergostero[J].Hebei Chemical Industry,2007,30(2):42-43.
[13] 任晓琼,赵风清,胡倩倩.利用青霉素废菌丝体制备脱硫石膏缓凝剂的研究[J].环境科学与技术,2014,37(2):134-137. REN Xiaoqiong,ZHAO Fengqing,HU Qianqian.Study on gypsum retarder from waste penicillin mycelium[J]. Environmental Science & Technology,2014,37(2):134-137.
[14] 苗雨田,杨悠悠,王浩,等.全自动氨基酸分析仪法测定不同年份黄酒中游离氨基酸的含量[J].食品安全质量检测学报,2015,6(4):1155-1159. MIAO Yutian,YANG Youyou,WANG Hao,et al.Determination of amino acids content in yellow rice wine of different years with automatic amino acid analyzer[J].Journal of Food Safety Quality,2015,6(4):1155-1159.
[15] 赵莹,梁骁男,李芹,等.高温缓凝剂LY的合成及在低密度水泥浆中的应用[J].化工进展,2015,34(8):3124-3127. ZHAO Ying,LIANG Xiaonan,LI Qin,et al.Synthesis of a high temperature retarder LY and the application in low density cement[J].Chemical Industry and Engineering Progress,2015,34(8):3124-3127.
[16] 彭家惠,王祖润,刘进超,等.复合型缓凝剂对脱硫建筑石膏水化进程的影响及缓凝机理[J].重庆大学学报,2011,34(11):73-75. PENG Jiahui,WANG Zurun,LIU Jinchao,et al.Influence of compound retarders on the hydration process of FGD building gypsum and its retarding mechanism[J].Journal of Chongqing University,2011,34(11):72-75.
[17] SINGH M,GARG M.Retarding action of various chemicals on setting and hardening characterings of gypsum plaster at different pH[J].Cement and Concrete Research,1997,27(6):947-950.
[18] KOJIMA Y,YASUE T.Synthesis of large plate-like gypsum dihydrate from waste gypsum board[J].Journal of the European Ceramic Society,2006,26(4/5):777-783.