Abstract: In pretreatments of lignocellulosic materials for bioethanol production, certain amount of sugars was degraded as fermentation inhibitors, and the degradation was much more severe in the acidic pretreatment.Theses fermentations inhibitors including formic acid, acetic acid, levulinic acid, furfural, and 5-hydroxymethylfurfural (HMF) which affect the conversion yield of ethanol from glucose fermentation.This paper examined the effect of the five fermentation inhibitors concentration on the ethanol yield of Saccharomyces cerevisiae glucose fermentation. The results show that, synergistic effect of various fermentation inhibitors on ethanol fermentation was higher than single fermentation inhibitor on ethanol fermentation.The total fermentation inhibitors in fermentation solutions generally controlled within 3.0g/L, which inhibitory effect on glucose fermentation for ethanol is not obvious.

Key words: fermentation inhibitors, ethanol fermentation, bioethanol, synergistic effect

摘要： 在纤维素乙醇研究中,木质纤维原料在酸性预处理过程中会产生甲酸、乙酸、乙酰丙酸、糠醛和5-羟甲基糠醛等发酵抑制物,这些发酵抑制物会影响葡萄糖发酵生产乙醇的收率。本文考察了发酵液中各种发酵抑制物含量对高温超级酿酒酵母发酵乙醇收率的影响。研究结果表明,多种发酵抑制物的协同作用对乙醇发酵的影响要高于单一种类发酵抑制物对乙醇发酵的影响。发酵液中发酵抑制物总量一般控制在3.0g/L以内时,对葡萄糖发酵生产乙醇的抑制作用不明显。

关键词: 发酵抑制物, 乙醇发酵, 生物乙醇, 协同作用