Abstract: To efficiently synthesize optically active chiral sulfoxides，Rhodococcus sp. CCZU10-1 was used for the asymmetric resolution of rac-PMSO into (S)-PMSO (ee > 99.9%) in aqueous single-phase system. After optimization，the optimum reaction pH，reaction temperature，shaker speed，co-substrate，cells dosage were 8.0，30℃，180 r/min，galactose (50mmol/L) and 0.08g (wet weight)/mL，respectively. By biotransformation of rac-PMSO (20mmol/L) for 6h under the optimized conditions，(R)-PMSO was completely oxidized into the corresponding sulfone，and (S)-PMSO (ee > 99.9%) was obtained at a high yield of 47.1%. These results would lay the foundation of industrial production of (S)-PMSO.

Key words: Rhodococcus sp., (S)-PMSO, resolution, biotransformation, optimization

摘要： 为了高效地合成高光学纯手性亚砜，在单水相体系中利用Rhodococcus sp. CCZU10-1选择性氧化拆分外消旋苯甲亚砜（rac-PMSO）合成了(S)-PMSO（ee>99.9%）。通过考察反应pH值、反应温度、摇床转速、辅助底物、生物催化剂添加量对催化反应的影响，确定了最适反应条件。结果表明最适反应条件为：反应pH值8.0、反应温度30℃、摇床转速180r/min、辅助底物为半乳糖（50mmol/L）、细胞浓度为0.08g(湿重)/mL。在最适反应条件下生物转化20mmol/L rac-PMSO时，(R)-PMSO完全转化，(S)-PMSO（ee>99.9%）产率为47.1%。因此，研究结果为工业化生产(S)-PMSO奠定了基础。

关键词: 红球菌属, (S)-苯甲亚砜, 拆分, 生物转化, 优化