Abstract: A new material，ALG-g-Lys，was used to prepare micro-beads instead of alginate to improve the stability of alginate micro-beads. The preparation process was optimized using the single factor method combined with orthogonal design. The optimized parameters were：ALG-g-Lys concentration 1.5g/L，voltage 8.0kV，advance speed 30mm/h，needle 24G，and CaCl2 concentration 15g/L. The prepared micro-beads showed even distribution and good sphericity with low trailing ratio，half sphere ratio and globule ratio. The mean particle size was 253±40μm. This work supplies a novel carrier material instead of alginate to prepare gel，showing bright future in medicine and food industries.

Key words: lysine grafted alginate, micro-bead, orthogonal design, optimization

摘要： 为了提高海藻酸钙胶珠的稳定性，采用海藻酸盐的改性材料ALG-g-Lys制备微胶珠，通过单因素考察结合正交设计优化了ALG-g-Lys微胶珠的制备工艺。优化后的制备工艺为：ALG-g-Lys 浓度为15g/L，电压为8.0kV，推进速度为30mm/h，针头型号为24G，CaCl2浓度为15g/L。优化后制得的微胶珠粒径均匀，球形度好，拖尾率、半球率和小球率小，平均粒径为253±40μm。该工作提供了一种新型的载体材料替代海藻酸盐作为凝胶材料，未来可适用于医药、食品行业等领域。

关键词: 赖氨酸接枝海藻酸盐, 微胶珠, 正交设计, 优化