Abstract: Synthetization of nicotine imprinted polymer (MIP)-derivatized silica monolithic column was carried out based on in situ polymerization on the surface of macroporous silica gel-chemically modified as the matrix. Structural characterization was performed by FTIR，SEM，and the total pores and microspores volume were measured though size exclusion chromatography. In HPLC system，chromatographic condition was tested. Frontal analysis was utilized for producing absorption isotherm for the MIP monolithic column. Additionally，adsorption thermodynamic behavior of the MIP was studied. The result showed that selectivity factors of nicotine were 4.31 and 2.95 relative to 8-hydroxyquinoline and pyridine respectively，and the optimized chromatographic conditions was pH=7.5. Freundlich model was shown better than Langmuir model to describe isotherm adsorption behavior of MIP monolith toward nicotine with a heterogeneity index of 0.841. Thermodynamic analysis indicated a spontaneous exothermic process for the adsorption of template on the MIP monolithic column.

Key words: Molecular imprinted monolith, In situ polymerization, Silica, Nicotine, Molecular recognition, molecular imprinted monolith, in situ polymerization, silica, nicotine, molecular recognition

摘要： 采用原位聚合并表面接枝制备了硅胶衍生尼古丁印迹整体柱。红外光谱和扫描电镜研究了聚合物的结构，基于尺寸排阻色谱原理测定了整体柱的总孔和微孔体积。在高效液相色谱系统中，考察了色谱条件对整体柱选择性的影响；用前沿色谱法测试了MIP整体柱的等温吸附，并研究了整体柱的吸附热力学。结果表明： MIP整体柱对尼古丁的选择因子相对8-羟基喹啉和吡啶分别为4.31和2.95；当流动相中pH值为7.5时，选择性最好；Freundlich模型比Langmuir模型更适合描述尼古丁在印迹整体柱上表面位点分布，位点异化指数为0.841；吸附热力学分析表明尼古丁在印迹整体柱上的自发放热吸附过程。

关键词: 分子印迹整体柱, 原位聚合, 硅胶, 尼古丁, 分子识别, 分子印迹整体柱, 原位聚合, 硅胶, 尼古丁, 分子识别