Application of novel molecularly imprinted biosensor in cancer marker

doi:10.16085/j.issn.1000-6613.2022-0617

Abstract

Abstract:

Cancer biomarkers, as specific measurable indicators in the body, can monitor cancer health through their own changes, and are of great significance for cancer prevention, early diagnosis and precise treatment. Therefore, rapid detection and precise analysis of typical disease markers have become a hot field of scientific research and clinical diagnosis. Molecular imprinting-based bio-sensing technology is expected to become a new method for rapid detection of disease markers due to its high sensitivity, fast response, strong specificity and low cost, and has been widely studied in the field of cancer clinical analysis. This review introduces the molecularly imprinted polymer synthetic methods and strategies, the sensor technology and means through the combination of molecular imprinting with electrochemical, surface plasma resonance and optical analysis, and the application of molecularly imprinted sensor in prostate cancer, breast cancer, liver cancer and other cancers biomarkers. Finally, this review summarizes the application status of molecularly imprinted sensors in the analysis and detection of cancer biomarkers at home and abroad, and puts forward the prospect of its development in the future.

Key words: molecular imprinting, electrochemistry, bio-sensing, cancer biomarker, early diagnosis, detection

摘要：

癌症生物标志物作为一种特定的身体中可测量的指标，可以通过自身产生的变化来监测癌症健康状况，对于癌症的预防、早期诊断和精准治疗具有重要意义。因此，面向典型疾病标志物的快速检测与精准分析已成为科学研究和临床诊断的热点领域。基于分子印迹的生物传感器由于其灵敏度高、响应速度快、特异性强以及成本低等优点，有望成为癌症生物标志物快速检测的新手段，在癌症临床分析领域得到了广泛研究。本综述简要介绍了分子印迹聚合物的合成方法和策略，分子印迹与电化学、表面等离子共振和光学分析集成的传感技术和手段，以及分子印迹生物传感在癌症生物标志物的应用。最后本综述就目前国内外关于分子印迹传感器在分析检测癌症生物标志物领域的应用现状进行了总结和讨论，并展望了其未来发展前景。

关键词: 分子印迹, 电化学, 生物传感, 癌症生物标志物, 早期诊断, 检测

CLC Number:

O657.1

LUO Aiqin, CAI Yanhui, LIANG Axin, XIE Bingteng. Application of novel molecularly imprinted biosensor in cancer marker[J]. Chemical Industry and Engineering Progress, 2022, 41(S1): 448-460.

罗爱芹, 蔡艳慧, 梁阿新, 解炳腾. 新型分子印迹生物传感器在癌症生物标志物中的应用[J]. 化工进展, 2022, 41(S1): 448-460.

Figures/Tables 6

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癌症类型	生物标志物	技术	线性范围	检出限	参考文献
前列腺癌	PSA	SPR	0.1~50×10^-9g/mL	91×10^-12g/mL	［98］
		电化学	0.01~4×10^-9g/mL	2×10^-12g/mL	［79］
		SPR	—	—	［99］
	SAR	电化学	5.0~1100×10^-6mol/L	0.38×10^-6mol/L	［81］
	SAR	电化学	1~100pm	0.4pm	［82］
乳腺癌	HER2-ECD	电化学	10~70×10^-9g/mL	1.6×10^-9g/mL	［84］
		电化学	5~50U/mL	1.5U/mL	［86］
	CA 15-3	电化学	1.44~13.2U/mL	1.07U/mL	［87］
		光学	0.0005~40U/mL	50μU/mL	［100］
		电化学	0.1~100×10^-6g/mL	0.08×10^-9g/mL	［90］
	CEA	电化学	—	0.1×10^-12g/mL	［91］
		—	1~500×10^-9g/mL	0.32×10^-9g/mL	［92］
肝癌	AFP	光学	0.025~100×10^-9g/mL	2.5×10^-12g/mL	［105］
			0.01~100×10^-9g/mL	1.2×10^-12g/mL
			0.001~25×10^-9g/mL	0.05×10^-12g/mL
			0.005~25×10^-9g/mL	0.015×10^-12g/mL
		光学	20~125μg/L	9.65μg/L	［107］
		光学	10~100×10^-9g/mL	0.474×10^-9g/mL	［108］
其他	Anti SARS-CoV-2	电化学	111×10^-15mol/L	15×10^-15mol/L	［97］
	IL-2	光学	35×10^-15g/mL~39×10^-12g/mL	5.91×10^-15g/mL	［110］

癌症类型	生物标志物	技术	线性范围	检出限	参考文献
前列腺癌	PSA	SPR	0.1~50×10^-9g/mL	91×10^-12g/mL	［98］
		电化学	0.01~4×10^-9g/mL	2×10^-12g/mL	［79］
		SPR	—	—	［99］
	SAR	电化学	5.0~1100×10^-6mol/L	0.38×10^-6mol/L	［81］
	SAR	电化学	1~100pm	0.4pm	［82］
乳腺癌	HER2-ECD	电化学	10~70×10^-9g/mL	1.6×10^-9g/mL	［84］
		电化学	5~50U/mL	1.5U/mL	［86］
	CA 15-3	电化学	1.44~13.2U/mL	1.07U/mL	［87］
		光学	0.0005~40U/mL	50μU/mL	［100］
		电化学	0.1~100×10^-6g/mL	0.08×10^-9g/mL	［90］
	CEA	电化学	—	0.1×10^-12g/mL	［91］
		—	1~500×10^-9g/mL	0.32×10^-9g/mL	［92］
肝癌	AFP	光学	0.025~100×10^-9g/mL	2.5×10^-12g/mL	［105］
			0.01~100×10^-9g/mL	1.2×10^-12g/mL
			0.001~25×10^-9g/mL	0.05×10^-12g/mL
			0.005~25×10^-9g/mL	0.015×10^-12g/mL
		光学	20~125μg/L	9.65μg/L	［107］
		光学	10~100×10^-9g/mL	0.474×10^-9g/mL	［108］
其他	Anti SARS-CoV-2	电化学	111×10^-15mol/L	15×10^-15mol/L	［97］
	IL-2	光学	35×10^-15g/mL~39×10^-12g/mL	5.91×10^-15g/mL	［110］

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