Process optimization and design of eliminating domestic agar phosphate precipitation

doi:10.16085/j.issn.1000-6613.2021-1682

Abstract

Abstract:

Agar is an important gel matrix for the preparation of microbial medium. Currently, phosphate precipitation is an inevitable problem in the preparation of microbial media using domestic agar, which has effected the accuracy of microbial experiments such as colony calculation. In order to solve this problem, we took turbidity and transparency as evaluation indexes to compare the elimination effects of adsorption method, masking agent method and chemical precipitation method on phosphate precipitation of Sigma agar, domestic agar strip and domestic agar powder, designed the optimized process and analyzed its economic feasibility. The experimental results showed that adsorption method and masking agent method had a certain impact on the transparency and turbidity of the three agars, but the quality of the products could not meet the standard of microbial culture medium configuration. Sigma agar and domestic agar strips were treated by potassium dihydrogen phosphate precipitation method, the agar transparency was increased to more than 94%, and the turbidity after phosphate treatment was reduced to 20NTU, which met the biochemical agar product standard for microbial culture medium. The process of treating domestic agar strips by potassium dihydrogen phosphate precipitation method was designed. The results showed that the total investment profit rate of domestic biochemical agar with an annual output of 1000t was 64.01%, and the static investment payback period was 3.3 years. The results showed that the treatment of domestic agar strips by potassium dihydrogen phosphate precipitation method could significantly eliminate phosphate precipitation, the product quality meets the configuration requirements of microbial culture medium, and it is economically feasible, which provides a technical reference for the removal of domestic agar phosphate precipitation and the production of agar products of microbial culture medium.

Key words: agar, phosphate precipitation, transparency, turbidity, design

摘要：

琼脂是配制微生物培养基的重要凝胶基质，目前国产琼脂在配制培养基时具有显著的磷酸盐沉淀现象，影响了菌落计算等微生物实验的准确性。为了消除磷酸盐沉淀，本实验以添加磷酸盐后琼脂浊度及透明度为评价指标，研究吸附法、掩蔽剂法和化学沉淀法对Sigma琼脂、国产琼脂条和国产琼脂粉的磷酸盐沉淀的消除效果，并对优化工艺进行设计及经济可行性分析。实验结果显示，吸附法、掩蔽剂法对三种琼脂的透明度和浊度都具有一定的影响，但产品的质量达不到微生物培养基配置的标准。磷酸二氢钾沉淀法处理Sigma琼脂和国产琼脂条，琼脂透明度提升至94%以上，磷酸盐处理浊度降至20NTU，符合微生物培养基所用的生化琼脂产品标准。对磷酸二氢钾沉淀法处理国产琼脂条的工艺进行设计，结果显示年产1000t国产消除磷酸盐沉淀的生化琼脂总投资利润率为64.01%，静态投资回收期为3.3年。本文结果显示磷酸二氢钾沉淀法处理国产琼脂条可以显著消除磷酸盐沉淀，生产的产品质量符合微生物培养基配置要求，且经济上具有可行性，为国产化去除琼脂磷酸盐沉淀、生产微生物培养基的琼脂产品提供了技术参考。

关键词: 琼脂, 磷酸盐沉淀, 透明度, 浊度, 设计

CLC Number:

TQ281

DUAN Boyan, NI Hui, LI Zhipeng, JIANG Zedong, ZHU Yanbing, LI Qingbiao. Process optimization and design of eliminating domestic agar phosphate precipitation[J]. Chemical Industry and Engineering Progress, 2022, 41(7): 3824-3831.

段博严, 倪辉, 李志朋, 姜泽东, 朱艳冰, 李清彪. 消除国产琼脂磷酸盐沉淀的工艺优化及设计[J]. 化工进展, 2022, 41(7): 3824-3831.

Figures/Tables 7

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类别	名称	规格	单价 /万元	数量	金额 /万元
合计	—				203.48
机器设备	蒸煮锅	2400×3500（mm）	0.85	8	6.8
	板框压滤机	4000×1200×1100（mm）	1.86	4	7.44
	置物架	4500×4000×2000（mm）	0.5	8	4
	三足离心机	3000×1200（mm）	1.48	8	11.84
	浸洗池	2200×1200×1000（mm）	0.12	12	1.44
	高速粉碎机	600×750×1450（mm）	1.68	4	6.72
	物料泵	300L/min	1.27	12	15.24
建筑工程	生产车间	20×50（m）	0.15	1000m²	150

类别	名称	规格	单价 /万元	数量	金额 /万元
合计	—				203.48
机器设备	蒸煮锅	2400×3500（mm）	0.85	8	6.8
	板框压滤机	4000×1200×1100（mm）	1.86	4	7.44
	置物架	4500×4000×2000（mm）	0.5	8	4
	三足离心机	3000×1200（mm）	1.48	8	11.84
	浸洗池	2200×1200×1000（mm）	0.12	12	1.44
	高速粉碎机	600×750×1450（mm）	1.68	4	6.72
	物料泵	300L/min	1.27	12	15.24
建筑工程	生产车间	20×50（m）	0.15	1000m²	150

类别	项目	用量	单价/元	合计/万元
水电汽耗	水耗	73920.6t	4	29.57
	电耗	44226kW·h	0.5	2.21
	汽耗	842.4t	201.5	16.97
原材料消耗	国产琼脂条	1053t	130000	13689
原材料消耗	磷酸氢二钾	350964.9kg	4.8	168.46
易耗品	500g塑料瓶	2000000个	0.68	136
	2号纸箱	100000个	1.0	10
	滤袋	100个	23.9	0.24
其他	维修费	—	—	5.27
	设备折旧费	—	—	5.08
	销售费用	—	—	240
	商检费用	—	—	48
	人力成本	—	—	157.95
合计	—			14508.75

类别	项目	用量	单价/元	合计/万元
水电汽耗	水耗	73920.6t	4	29.57
	电耗	44226kW·h	0.5	2.21
	汽耗	842.4t	201.5	16.97
原材料消耗	国产琼脂条	1053t	130000	13689
原材料消耗	磷酸氢二钾	350964.9kg	4.8	168.46
易耗品	500g塑料瓶	2000000个	0.68	136
	2号纸箱	100000个	1.0	10
	滤袋	100个	23.9	0.24
其他	维修费	—	—	5.27
	设备折旧费	—	—	5.08
	销售费用	—	—	240
	商检费用	—	—	48
	人力成本	—	—	157.95
合计	—			14508.75

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