Toxic effect of benzothiazole ionic liquids on antioxidation mechanism of zebrafish

doi:10.16085/j.issn.1000-6613.2016.s2.046

Abstract

Abstract: With the wide application and development of ionic liquids,the effect of their toxicity gained more attention.Among the kinds of ionic liquids,the benzothiazole ionic liquids are on the period of initial development,and the study of toxicity has to be perfect.Zebrafish are mark of water contamination monitor,and applied to test the safety of aqueous solution.This study used the SOD activity from antioxidation mechanism of zebrafish as index,chose the method of semi-static contamination aqueous solution to get the SOD activity of liver tissue,and explore the effect of acid and neutral ionic liquid on antioxidation mechanism of aquatic organism.The results proved that the benzothiazole ionic liquids lead the SOD activity of liver to decrease,and took lethal effect on zebrafish.Besides the acid benzothiazole ionic liquid showed stronger toxicity than neutral,and the same anion ionic liquid had the same level of toxicity.

Key words: ionic liquid, zebrafish, toxicity, SOD

摘要： 随着离子液体的应用和发展不断推进，其毒性影响得到广泛关注，其中苯并噻唑离子液体仍处于初步发展阶段，其毒性方面的研究还有待完善。斑马鱼作为水质污染的生物指标，广泛应用于评测水体安全的研究中。本文选用斑马鱼的抗氧化机制中SOD酶活性作为测定指标，采用半静态水体染毒法取得染毒鱼体的肝组织，对其SOD活性进行测定，得到酸性及中性苯并噻唑类离子液体对水生生物的抗氧化机制的影响，及对水生生物的毒性影响。研究结果表明，苯并噻唑类离子液体致使斑马鱼肝组织内的SOD活性呈现下降趋势，对斑马鱼产生致死毒性影响，其中酸性苯并噻唑离子液体对斑马鱼具有更显著的毒性影响，而相同阴离子的酸性和中性离子液体有相同的毒性水平。

关键词: 离子液体, 斑马鱼, 毒性, 超氧化物歧化酶

CLC Number:

TQ460

CHEN Yanwen, LUO Yingjie, YANG Xiaoxue, YAO Shun, SONG Hang. Toxic effect of benzothiazole ionic liquids on antioxidation mechanism of zebrafish[J]. Chemical Industry and Engineering Progree, 2016, 35(S2): 276-278.

陈彦文, 罗英杰, 杨小雪, 姚舜, 宋航. 苯并噻唑类离子液体对斑马鱼抗氧化机制的毒性影响[J]. 化工进展, 2016, 35(S2): 276-278.

References

[1] 柯明,周爱国,宋昭峥,等.离子液体的毒性[J].化学进展,2007,19(5):671-679.
[2] 赵继红,赵永升,张宏忠,等.咪唑类离子液体毒性的QSAR/QSPR研究[J].环境科学,2013,34(5):1882-1886.
[3] 牧辉,彭新晶,戴宁,等.离子液体[C₈mim]PF₆对水生生物的毒性作用[J].中国环境科学,2009,29(11):1196-1201.
[4] 刘在平,张松林.斑马鱼在环境监测中的应用[J].江苏农业科学,2011,39(2):486-488.
[5] 李洁斐,李卫华,金泰廙,等.斑马鱼及其在环境毒理学中的应用[J].环境与职业医学,2005,22(5):460-463.
[6] HALLARE A V,SCHIRLING M,LUCKENBACH T,et al. Combined effects of temperature and cadmium on developmental parameters and biomarker responses in zebrafish(Danio rerio)embryos[J].Journal of Thermal Biology,2005,30(1):7-17.
[7] DONG M,ZHU L,ZHU S,et al.Toxic effects of 1-decyl-3-methylimidazolium bromide ionic liquid on the antioxidant enzyme system and DNA in zebrafish(Danio rerio)livers[J]. Chemosphere,2013,91(8):1107-1112.
[8] ZHEN H,WEN M,YANG Y,et al.Toxic effects of HgCl₂ on activities of SOD,AChE and relative expression of SOD,AChE,CYP1A1 of zebrafish[J].Ecotoxicology,2014,23(10):1842-1845.
[9] 罗帅,李玉文,马玲,等.离子液体对斑马鱼急性毒性及保护酶活性的影响[J].安徽农业科学,2014(3):786-789.
[10] 郭莹莹.四种咪唑类离子液体对斑马鱼的急性毒性、氧化损伤及基因毒性[D].泰安:山东农业大学,2015.
[11] 彭琦,张义文,王小敏,等.新型苯并噻唑类离子液体的合成及其理化性质研究[J].合成化学,2012,20(1):28-31.
[12] PRETTI C,CHIAPPE C,PIERACCINI D,et al.Acute toxicity of ionic liquids to the zebrafish(Danio rerio)[J]. Green Chemistry,2006,8(3):238-240.
[13] 蔡道基,杨佩芝,龚瑞忠,等.化学农药环境安全评价试验准则[S].北京:国家环保局,1989.
[14] 国家环境保护局.化学农药环境安全评价试验准则[S].北京:国家环境保护局,1989.

[1]	HU Xi, WANG Mingshan, LI Enzhi, HUANG Siming, CHEN Junchen, GUO Bingshu, YU Bo, MA Zhiyuan, LI Xing. Research progress on preparation and sodium storage properties of tungsten disulfide composites [J]. Chemical Industry and Engineering Progress, 2023, 42(S1): 344-355.
[2]	WANG Min, MAO Yuhong, CHEN Chao, BAI Dan. Progress on the toxicity, morphology and control of aluminum salt hydrolysates in water treatment process [J]. Chemical Industry and Engineering Progress, 2023, 42(S1): 479-488.
[3]	ZHANG Qi, ZHAO Hong, RONG Junfeng. Research progress of anti-toxicity electrocatalysts for oxygen reduction reaction in PEMFC [J]. Chemical Industry and Engineering Progress, 2023, 42(9): 4677-4691.
[4]	WANG Shaofan, ZHOU Ying, HAO Kang’an, HUANG Anrong, ZHANG Ruju, WU Chong, ZUO Xiaoling. Self-healing and blue-light hydrogel with pH responsiveness [J]. Chemical Industry and Engineering Progress, 2023, 42(9): 4837-4846.
[5]	YANG Han, ZHANG Yibo, LI Qi, ZHANG Jun, TAO Ying, YANG Quanhong. Practical carbon anodes for sodium-ion batteries: progress and challenge [J]. Chemical Industry and Engineering Progress, 2023, 42(8): 4029-4042.
[6]	WU Ya, ZHAO Dan, FANG Rongmiao, LI Jingyao, CHANG Nana, DU Chunbao, WANG Wenzhen, SHI Jun. Research progress on highly efficient demulsifiers for complex crude oil emulsions and their applications [J]. Chemical Industry and Engineering Progress, 2023, 42(8): 4398-4413.
[7]	YANG Xuzhao, LI Qing, YUAN Kangkang, ZHANG Yingying, HAN Jingli, WU Shide. Thermodynamic properties of Gemini ionic liquid based deep eutectic solvents [J]. Chemical Industry and Engineering Progress, 2023, 42(6): 3123-3129.
[8]	LYU Chao, ZHANG Xiwen, JIN Lijian, YANG Linjun. Efficient capture of CO₂ by a new biphasic solvent-ionic liquid system [J]. Chemical Industry and Engineering Progress, 2023, 42(6): 3226-3232.
[9]	CHEN Yu, LIU Chong, QIU Yuhui, BI Zixin, MU Tiancheng. Ionic liquids and deep eutectic solvents for green recycle of spent lithium-ion batteries [J]. Chemical Industry and Engineering Progress, 2022, 41(S1): 485-496.
[10]	HUA Qucheng, DUAN Qinghua. Research progress of ionic liquid as extreme pressure and anti-wear agent [J]. Chemical Industry and Engineering Progress, 2022, 41(S1): 331-339.
[11]	HAN Mingyang, QIAO Hui, FU Jiaming, MA Zewen, WANG Yan, OUYANG Jia. Research progress of non-aqueous solvents on the pretreatment of lignocellulose [J]. Chemical Industry and Engineering Progress, 2022, 41(8): 4086-4097.
[12]	SHAN Qingwen, ZHANG Juan, WANG Yajuan, LIU Wenqiang. Synthesis of polymeric ionic liquid and its performance on adsorption desulfurization [J]. Chemical Industry and Engineering Progress, 2022, 41(8): 4571-4579.
[13]	YUAN Xiaolu, LI Baoxia, HUANG Yayan, YANG Yucheng, YE Jing, ZHANG Na, ZHANG Xueqin, ZHENG Bingde, XIAO Meitian. Progress in preparation and application of sodium alginate microcapsules [J]. Chemical Industry and Engineering Progress, 2022, 41(6): 3103-3112.
[14]	ZHANG Ningning, DING Hua, GAO Yan, BAI Xiangfei, ZHANG Yunpeng, SUN Nanxiang. Migration behavior of sodium in Xinjiang Naomaohu coal during the CO₂ gasification [J]. Chemical Industry and Engineering Progress, 2022, 41(5): 2348-2355.
[15]	LI Po, ZHANG Shanshan, SHI Jinqiu, GAO Hang, WANG Mingxin. Remediation of aniline-contaminated groundwater by activated persulfate and its environmental risks [J]. Chemical Industry and Engineering Progress, 2022, 41(5): 2753-2760.