Ultrafiltration preprocessing and continuous separation process of single-component polysaccharides from Taxus Mairei

doi:10.16085/j.issn.1000-6613.2015.02.032

Chemical Industry and Engineering Progree ›› 2015, Vol. 34 ›› Issue (02): 508-513.DOI: 10.16085/j.issn.1000-6613.2015.02.032

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Ultrafiltration preprocessing and continuous separation process of single-component polysaccharides from Taxus Mairei

ZHANG Feifei^1,2, TIAN Hui², LIANG Yafei³, WU Mianbin^1,2, LIN Jianping^1,2, YANG Lirong^1,2

1. Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang University, Hangzhou 310027, Zhejiang, China;
2. Department of Chemical and Biolaogical Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China;
3. Jinhua People's Hospital, Jinhua 321000, Zhejiang, China

Received:2014-07-03 Revised:2014-08-08 Online:2015-02-05 Published:2015-02-05

红豆杉单组分多糖的超滤预处理及连续分离工艺

张菲菲^1,2, 田慧², 梁亚非³, 吴绵斌^1,2, 林建平^1,2, 杨立荣^1,2

1. 浙江大学生物质化工教育部重点实验室, 浙江杭州 310027;
2. 浙江大学化学工程与生物工程学系, 浙江杭州 310027;
3. 金华市人民医院, 浙江金华 318100

通讯作者: 吴绵斌,副教授,E-mail:wumb@zju.edu.cn;林建平,教授,E-mail:linjp@zju.edu.cn
作者简介:张菲菲(1989-),女,硕士研究生,E-mail:857307211@qq.com;田慧(1983-),女,硕士研究生,E-mail:1031539118@qq.com田慧和张菲菲对本篇论文具有相同贡献
基金资助:
国家自然科学基金(21376215)、浙江省科技计划(2010C33122)、国家973计划(2011CB710800)及国家863计划(2012AA022302)项目

Abstract

Abstract: The total polysaccharides from Taxus Mairei were pretreated by ultrafiltration to remove pigments and the operating condition was optimized as follows:molecular cut-off 5 kDa,temperature 25℃,operating pressure 20psi and pH6.8. Under the aforementioned condition,the 1L solution containing 10g/L total polysaccharides was ultrafiltrated to 125mL first,then deionized water was added into condensed solution to 1L total volume and was repeated 4 times,respectively. The results showed that the retention rate and decolourization ratio of polysaccharides of Taxus Mairei were 87.2% and 75.6%,respectively. Furthermore,the pretreated total polysaccharides of Taxus Mairei were isolated by cation-exchange chromatography using a self-designed continuous chromatography equipment,the processing capacity of which reached 100.08L/d. Based on the operating parameters received from batch chromatography process,99.0% purity single-component polysaccharide (PST-1) was obtained and the average yield of PST-1 reached 50.0%.

Key words: Taxus Mairei, polysaccharides, ultrafiltration, continuous chromatography separation

摘要： 以红豆杉枝叶部位总多糖为原料,采用超滤方法对原料进行预处理,并对工艺进行优化,最佳优化条件为:截留相对分子质量为5kDa,温度为25℃,压力为20psi(1psi=6894.76kPa),pH值为6.8。在此条件下,将浓度为10g/L的1L红豆杉多糖溶液超滤至125mL,再添加去离子水至1L,反复超滤4次,得到的红豆杉多糖的截留率和脱色率分别为87.2%和75.6%。进一步以红豆杉单组分多糖间歇色谱分离工艺参数为基础,采用自行设计制作的连续色谱设备,建立了连续离子交换色谱分离红豆杉酸性单组分多糖PST-1的分离工艺,处理量达到每批次100.08L,PST-1纯度在99.0%以上,收率达到50.0%。

关键词: 红豆杉, 多糖, 超滤, 连续色谱分离

CLC Number:

R284.2

ZHANG Feifei, TIAN Hui, LIANG Yafei, WU Mianbin, LIN Jianping, YANG Lirong. Ultrafiltration preprocessing and continuous separation process of single-component polysaccharides from Taxus Mairei[J]. Chemical Industry and Engineering Progree, 2015, 34(02): 508-513.

张菲菲, 田慧, 梁亚非, 吴绵斌, 林建平, 杨立荣. 红豆杉单组分多糖的超滤预处理及连续分离工艺[J]. 化工进展, 2015, 34(02): 508-513.

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Ultrafiltration preprocessing and continuous separation process of single-component polysaccharides from Taxus Mairei

红豆杉单组分多糖的超滤预处理及连续分离工艺

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