化工进展 ›› 2021, Vol. 40 ›› Issue (4): 2234-2242.DOI: 10.16085/j.issn.1000-6613.2020-1022

• 材料科学与技术 • 上一篇    下一篇

盐响应型蛋白质吸附阳离子交换膜

向燕1(), 谢锐1,2(), 巨晓洁1,2, 汪伟1,2, 刘壮1,2, 褚良银1,2   

  1. 1.四川大学化学工程学院,四川 成都 610065
    2.四川大学高分子材料工程国家重点实验室,四川 成都 610065
  • 收稿日期:2020-06-08 出版日期:2021-04-05 发布日期:2021-04-14
  • 通讯作者: 谢锐
  • 作者简介:向燕(1995—),女,硕士研究生,研究方向为膜材料与膜技术。E-mail:scu_xiangyan@163.com
  • 基金资助:
    国家自然科学基金(21622604);四川省杰出青年科技人才项目(2019JDJQ0026)

Salt-responsive cation exchange membrane for protein adsorption

XIANG Yan1(), XIE Rui1,2(), JU Xiaojie1,2, WANG Wei1,2, LIU Zhuang1,2, CHU Liangyin1,2   

  1. 1.School of Chemical Engineering, Sichuan University, Chengdu 610065, Sichuan, China
    2.State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, Sichuan, China
  • Received:2020-06-08 Online:2021-04-05 Published:2021-04-14
  • Contact: XIE Rui

摘要:

通过简单可控的“一锅法”将聚甲基丙烯酸-3-磺酸丙酯钾盐(PSPM)接枝到化学稳定性良好的富含叔胺基的聚醚砜酮(PTA)膜表面,制备得到盐响应型阳离子交换膜(PTA-GS)。以中性时带正电的溶菌酶(Lys)和带负电的牛血清白蛋白(BSA)作为模型蛋白质,系统研究了PTA-GS膜对蛋白质的静态吸附性能、吸附动力学以及盐响应的吸附性能,并采用等温吸附模型、吸附动力学模型对实验结果进行拟合。结果表明,PTA-GS膜对Lys的吸附更符合Redlich-Peterson、Langmuir等温吸附模型和拟二级动力学方程。在25℃,中性缓冲溶液中PTA-GS膜对Lys和BSA的平衡吸附量具有显著差异,它们的饱和吸附量分别为(174.2±22.8)mg/g和(8.1±2.5)mg/g。PTA-GS膜对Lys的吸附量随盐浓度的升高而降低,其在含1mol/L氯化钠的缓冲溶液中再生后蛋白质脱附率可达79.3%。研究结果可为简单制备吸附性能优异的盐响应型离子交换膜提供新思路,并为阳离子交换膜上蛋白质的吸附行为研究提供指导。

关键词: 聚强电解质, 盐响应型智能膜, 蛋白质, 吸附, 动力学模型

Abstract:

A salt-responsive cation exchange membrane (PTA-GS) was developed by grafting poly(3-sulfopropyl methacrylate potassium salt) (PSPM) onto the surface of polyethersulfone ketone membrane containing abundant tertiary amines (PTA) with excellent chemical stability via a simple and controllable “one-pot” method. The positive-charged lysozyme (Lys) and negative bovine serum albumin (BSA) in neutral environment were used as model proteins, and the static adsorption, adsorption kinetics and the salt-responsive adsorption performance of the PTA-GS membrane toward protein were studied systematically. Moreover, the experimental results were fitted by isothermal adsorption model and adsorption kinetics model. The results showed that the Lys adsorption on PTA-GS membrane was in line with Redlich-Peterson and Langmuir isothermal adsorption models, and pseudo-second-order kinetic equation. The results discovered that the equilibrium adsorption capacity of Lys and BSA on PTA-GS membrane was much different in neutral buffer solution at 25℃, and their saturated adsorption capacity were (174.2±22.8)mg/g and (8.1±2.5)mg/g, respectively. The adsorption capacity of PTA-GS membrane toward Lys declined with the increase of salt concentration, and the desorption ratio of protein reached 79.3% after regeneration in buffer solution with 1mol/L sodium chloride. The results provided a new strategy for the simple preparation of salt-responsive cation exchange membrane with excellent adsorption performance and guidance for the protein adsorption behavior on the cation exchange membrane.

Key words: strong polyelectrolyte, salt-responsive smart membrane, protein, adsorption, kinetic modeling

中图分类号: 

京ICP备12046843号-2;京公网安备 11010102001994号
版权所有 © 《化工进展》编辑部
地址:北京市东城区青年湖南街13号 邮编:100011
电子信箱:hgjz@cip.com.cn
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持:support@magtech.com.cn