盐响应型蛋白质吸附阳离子交换膜

doi:10.16085/j.issn.1000-6613.2020-1022

摘要/Abstract

摘要：

通过简单可控的“一锅法”将聚甲基丙烯酸-3-磺酸丙酯钾盐（PSPM）接枝到化学稳定性良好的富含叔胺基的聚醚砜酮（PTA）膜表面，制备得到盐响应型阳离子交换膜（PTA-GS）。以中性时带正电的溶菌酶（Lys）和带负电的牛血清白蛋白（BSA）作为模型蛋白质，系统研究了PTA-GS膜对蛋白质的静态吸附性能、吸附动力学以及盐响应的吸附性能，并采用等温吸附模型、吸附动力学模型对实验结果进行拟合。结果表明，PTA-GS膜对Lys的吸附更符合Redlich-Peterson、Langmuir等温吸附模型和拟二级动力学方程。在25℃，中性缓冲溶液中PTA-GS膜对Lys和BSA的平衡吸附量具有显著差异，它们的饱和吸附量分别为(174.2±22.8)mg/g和(8.1±2.5)mg/g。PTA-GS膜对Lys的吸附量随盐浓度的升高而降低，其在含1mol/L氯化钠的缓冲溶液中再生后蛋白质脱附率可达79.3%。研究结果可为简单制备吸附性能优异的盐响应型离子交换膜提供新思路，并为阳离子交换膜上蛋白质的吸附行为研究提供指导。

关键词: 聚强电解质, 盐响应型智能膜, 蛋白质, 吸附, 动力学模型

Abstract:

A salt-responsive cation exchange membrane (PTA-GS) was developed by grafting poly(3-sulfopropyl methacrylate potassium salt) (PSPM) onto the surface of polyethersulfone ketone membrane containing abundant tertiary amines (PTA) with excellent chemical stability via a simple and controllable “one-pot” method. The positive-charged lysozyme (Lys) and negative bovine serum albumin (BSA) in neutral environment were used as model proteins, and the static adsorption, adsorption kinetics and the salt-responsive adsorption performance of the PTA-GS membrane toward protein were studied systematically. Moreover, the experimental results were fitted by isothermal adsorption model and adsorption kinetics model. The results showed that the Lys adsorption on PTA-GS membrane was in line with Redlich-Peterson and Langmuir isothermal adsorption models, and pseudo-second-order kinetic equation. The results discovered that the equilibrium adsorption capacity of Lys and BSA on PTA-GS membrane was much different in neutral buffer solution at 25℃, and their saturated adsorption capacity were (174.2±22.8)mg/g and (8.1±2.5)mg/g, respectively. The adsorption capacity of PTA-GS membrane toward Lys declined with the increase of salt concentration, and the desorption ratio of protein reached 79.3% after regeneration in buffer solution with 1mol/L sodium chloride. The results provided a new strategy for the simple preparation of salt-responsive cation exchange membrane with excellent adsorption performance and guidance for the protein adsorption behavior on the cation exchange membrane.

Key words: strong polyelectrolyte, salt-responsive smart membrane, protein, adsorption, kinetic modeling

中图分类号:

TQ028.8

向燕, 谢锐, 巨晓洁, 汪伟, 刘壮, 褚良银. 盐响应型蛋白质吸附阳离子交换膜[J]. 化工进展, 2021, 40(4): 2234-2242.

XIANG Yan, XIE Rui, JU Xiaojie, WANG Wei, LIU Zhuang, CHU Liangyin. Salt-responsive cation exchange membrane for protein adsorption[J]. Chemical Industry and Engineering Progress, 2021, 40(4): 2234-2242.

图/表 9

图1 PTA膜和PTA-GS膜的SEM照片和表面水接触角

图2 PTA膜和PTA-GS膜的XPS全谱图

图3 初始浓度和吸附时间对PTA-GS膜蛋白质吸附量的影响（pH=7.2，T=25℃）

图4 PTA-GS膜吸附蛋白质的等温吸附模型

表1 三种等温吸附模型的模型参数

蛋白质	Freundlich模型				Langmuir模型				Redlich-Peterson模型
蛋白质	R²	χ²	K_F	n	R²	χ²	q_m	K_L	R²	χ²	A
Lys	0.9764	2.1665	187.7	3.27	0.9938	0.4975	232.1	4.30	0.9962	0.3674	746.05
BSA	0.9703	0.1006	6.3	3.45	0.9921	0.0244	8.2	3.75	0.9978	0.0072	20.40

图5 PTA-GS膜吸附蛋白质的动力学模型

表2 两种动力学模型的模型参数表

蛋白质	拟一级动力学方程				拟二级动力学方程
	q_e	速率常数k₁		R²	q_e	速率常数k₂		R²
	q_e	拟合值	标准误差	R²	q_e	拟合值	标准误差	R²
Lys	233.8	0.3816	0.0452	0.9727	196.1	0.0014	0.0002	0.9966
BSA	7.7	0.1674	0.0096	0.9934	11.4	0.0123	0.0023	0.9866

图6 NaCl浓度对PTA-GS膜渗透通量和蛋白质吸附量的影响(T=25℃)

图7 PTA-GS膜吸附和脱附蛋白质的示意图

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