1,2,4-丁三醇的双水相萃取

doi:10.16085/j.issn.1000-6613.2019-1801

化工进展 ›› 2020, Vol. 39 ›› Issue (8): 3273-3277.DOI: 10.16085/j.issn.1000-6613.2019-1801

1,2,4-丁三醇的双水相萃取

杜宣慧¹(), 钱方圆¹, 陆信曜¹^,²^,³, 宗红¹^,³, 诸葛斌¹^,³()

^1.江南大学糖化学与生物技术教育部重点实验室，江苏无锡 214122
^2.非粮生物质酶解国家重点实验室，广西南宁 530255
^3.江南大学工业生物技术教育部重点实验室，生物工程学院，工业微生物研究中心，江苏无锡 214122

出版日期:2020-08-01 发布日期:2020-08-12
通讯作者: 诸葛斌
作者简介:杜宣慧（1995—），女，硕士研究生，研究方向为工业微生物学与酶工程。E-mail：17851307212@163.com。
基金资助:
国家自然科学基金青年科学基金(21708016)

Separation of 1,2,4-butanetriol by aqueous two-phase extraction

Xuanhui DU¹(), Fangyuan QIAN¹, Xinyao LU¹^,²^,³, Hong ZONG¹^,³, Bin ZHUGE¹^,³()

^1.Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China
^2.State Key Laboratory of Non-Food Biomass and Enzyme Technology, Nanning 530255, Guangxi, China
^3.Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Laboratory of Industrial Microorganisms, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China

Online:2020-08-01 Published:2020-08-12
Contact: Bin ZHUGE

摘要/Abstract

摘要：

对低分子有机溶剂/无机盐双水相体系萃取分离发酵液中1,2,4-丁三醇（1,2,4-butanetriol，BT）进行了深入研究。通过对不同双水相体系的筛选，最终选定无水乙醇/K₂HPO₄双水相体系来萃取分离BT。使用浊点法对以BT为溶剂的无水乙醇/K₂HPO₄双水相体系进行相图的绘制，发现在K₂HPO₄质量分数为19.83%~46.87%范围内均能成相。通过单因素实验，考察双水相体系中无水乙醇/K₂HPO₄质量分数、pH对BT在两相之间分配系数和萃取效率的影响，得到最佳萃取条件为：系统总量10g、pH 9.5，无水乙醇/K₂HPO₄的质量分数为28%/28%，分配系数和萃取效率分别可以达到18.35和95.87%。在最佳萃取条件下，进一步探究了放大实验对体系萃取效率的影响，发现其对分配系数和萃取效率影响较小，体系稳定性高，为工业提取发酵液中BT提供新思路。

关键词: 双水相体系, 1,2,4-丁三醇, 萃取分离

Abstract:

Aqueous two-phase systems composed of organic solvents and inorganic salts could be used to extract 1,2,4-butanetriol (BT) from fermentation broth. Through the screening of several aqueous two-phase systems, the absolute ethanol/K₂HPO₄ two-phase system was finally selected to separate BT. The absolute ethanol/K₂HPO₄ two-phase system was investigated in detail, including phase diagram, and effect of phase composition on partition and pH. The results indicated that the phase could be formed in the range of the mass fraction of K₂HPO₄ being 19.83%~46.87%. The highest partition coefficient (18.35) and recovery (95.87%) of BT were obtained with an aqueous two-phase system composed of the system total 10g, 28% (mass ratio) absolute ethanol, 28% (mass ratio) K₂HPO₄ and pH 9.5. Under the optimal extraction condition, the system was further subjected to amplification experiments. The result showed that the amplification experiment had less influence on the distribution coefficient and recovery, which proved that the absolute ethanol/K₂HPO₄ two-phase system had high stability and potential for industrial application.

Key words: aqueous two-phase system, 1,2,4-butanetriol, extraction separation

中图分类号:

Q503

杜宣慧, 钱方圆, 陆信曜, 宗红, 诸葛斌. 1,2,4-丁三醇的双水相萃取[J]. 化工进展, 2020, 39(8): 3273-3277.

Xuanhui DU, Fangyuan QIAN, Xinyao LU, Hong ZONG, Bin ZHUGE. Separation of 1,2,4-butanetriol by aqueous two-phase extraction[J]. Chemical Industry and Engineering Progress, 2020, 39(8): 3273-3277.

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1,2,4-丁三醇的双水相萃取

Separation of 1,2,4-butanetriol by aqueous two-phase extraction

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