关键词: 微藻培养, 普通小球藻, 曝气间隔, 细胞密度, 新型套管式光生物反应器

Abstract: High-density cell culture could reduce the cost of large-scale cultivation of microalgae for biodiesel. Aeration is one of the important factors in high-density culture of microalgae. Chlorella vulgaris was cultured for 15 days in a novel concentric-tube photobioreactor with BG11 medium. The effect of different aeration intervals on cell density of Chlorella vulgaris，pH and dissolved oxygen changes of medium was studied. Air was passed into the concentric-tube photobioreactor at aeration flow rate 10L/min for 0.5h each aeration. The aeration manner of the novel concentric-tube photobioreactor could exclude dissolved oxygen timely. When biomass accumulation was stable，the pH of culture medium was substantially constant；the cell density was 7.22×106 cells/mL at aeration interval 0.5h. Compared to the cell concentration at aeration interval 1h，1.5h，2h，the cell concentrations at aeration interval 0.5h increased by 9.56%，41.02%，122.1%，respectively. Therefore，with the decrease of aeration interval time，concentration of Chlorella vulgaris increased.

Key words: microalgae cultivation, Chlorella vulgaris, aeration interval, cell density, novel concentric-tube photobioreactor