Abstract:

Isomaltulose is a functional sweetener emerged in recent years and has wide application prospects in the food and chemical industries. Sucrose isomerase（SIase）is currently the most effective enzyme for isomaltulose industrial production. The research progress of the SIase producing strain，purification and characterization as well as protein structure and function is reviewed. The catalytic mechanism of SIase for isomaltulose and trehalulose production，and the application in isomaltulose production are also presented. The research perspectives of SIase are discussed，and the research emphasis should be placed on screening ideal microbe strains and fermentation process optimization to enhance SIase production.