Abstract: Using chitosan and sodium alginate as micro-carrier material，calcium-alginate-chitosan microcapsules were prepared by an impulsive electrostatic preparation process. Enzyme activity of lipase immobilized in these microcapsules under different preparation conditions was investigated. Results indicated that the enzyme activity were mainly affected by lipase/alginate ratio and alginate mass concentration. The highest enzyme activity was found with 15 mg/mL enzyme loading and regular spherical microcapsules were obtained with 10 mg/mL alginate mass concentration. The microcapsule membrane creation was affected by both the relative molar mass and mass concentration of chitosan，through which the enzyme activity was also influenced. The pH value of chitosan solution regulated the ionization state of functional groups in both chitosan and alginate molecules，and hence the electrostatic binding in membrane generation，the enzyme protein encapsulation rate，and finally the enzyme activity. A maximum enzyme activity of 187 IU/g was achieved ultimately under preparation conditions with 15 mg/mL of lipase/alginate ratio，10 mg/mL of alginate mass concentration，50kDa of chitosan relative molar mass and 1 mg/mL of chitosan mass concentration with pH 3.0.