化工进展 ›› 2020, Vol. 39 ›› Issue (1): 329-340.DOI: 10.16085/j.issn.1000-6613.2019-0607
收稿日期:
2019-04-16
出版日期:
2020-01-05
发布日期:
2020-01-14
通讯作者:
冯旭东
作者简介:
刘潇斐(1995—),女,硕士研究生,研究方向为酶工程。E-mail:基金资助:
Xiaofei LIU(),Liang ZHANG,Xudong FENG(),Chun LI
Received:
2019-04-16
Online:
2020-01-05
Published:
2020-01-14
Contact:
Xudong FENG
摘要:
糖基化是天然产物的一种重要基团修饰,主要通过尿苷二磷酸(UDP)-糖基转移酶催化实现,而UDP-糖基供体价格昂贵限制了其应用,构建UDP-糖基供体再生系统可以有效解决该问题。本文从天然产物的体外糖基化修饰入手,阐述了糖基化修饰对天然产物功能的调控作用,比较了目前的糖基化修饰方法,其中基于UDP-糖基转移酶的生物法具备了重要的产业化应用前景。接着总结了各种UDP-糖基供体的合成方式,概述了偶联蔗糖合酶或海藻糖合酶的基于UDP循环的UDP-糖基供体再生系统,重点描述了UDP循环体系在萜类、黄酮类及其他化合物体外糖基化修饰中的应用,从而高效合成具有高附加值的天然产物糖苷化合物。指出偶联蔗糖合酶和UDP-糖基转移酶的循环体系是今后天然产物糖苷化合物合成的重要方式。
中图分类号:
刘潇斐,张良,冯旭东,李春. 偶联尿苷二磷酸循环体系的天然产物体外糖基化修饰[J]. 化工进展, 2020, 39(1): 329-340.
Xiaofei LIU,Liang ZHANG,Xudong FENG,Chun LI. UDP regeneration system for in vitro glycosylation modification of natural products[J]. Chemical Industry and Engineering Progress, 2020, 39(1): 329-340.
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