Chemical Industry and Engineering Progress ›› 2021, Vol. 40 ›› Issue (3): 1187-1201.DOI: 10.16085/j.issn.1000-6613.2020-1944

• Special column:Green biomanufacturing • Previous Articles     Next Articles

Rapid construction and directed evolution of cell factories for carotenoid biosynthesis

WANG Ying1,2,3(), QU Junze1,2,3(), LIANG Nan1,2,3, HAO He1,2,3, YUAN Yingjin1,2,3()   

  1. 1.Frontier Science Center for Synthetic Biology (Ministry of Education), Tianjin University, Tianjin 300072, China
    2.Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, China
    3.School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
  • Received:2020-09-23 Online:2021-03-17 Published:2021-03-05
  • Contact: YUAN Yingjin

合成类胡萝卜素细胞工厂的快速构建和定向进化

王颖1,2,3(), 曲俊泽1,2,3(), 梁楠1,2,3, 郝鹤1,2,3, 元英进1,2,3()   

  1. 1.天津大学教育部合成生物学前沿科学中心,天津 300072
    2.天津大学系统生物工程教育部重点实验室,天津 300072
    3.天津大学化工学院,天津 300072
  • 通讯作者: 元英进
  • 作者简介:王颖(1983—),女,讲师,研究方向为合成生物学。E-mail:ying.wang@tju.edu.cn|曲俊泽(1995—),男,研究助理,研究方向为合成生物学。E-mail:18222962721@163.com
  • 基金资助:
    国家自然科学基金(21621004)

Abstract:

Carotenoids have brilliant colors and strong antioxidant activity, which not only endow these compounds with high commercial value, but also accelerate the construction process for building efficient biosynthetic pathways in heterogeneous chassis. Carotenoids have become an ideal object to develop irrational design strategy. Here we critically review recent advances about carotenoid biosynthesis via irrational design strategies on pathway construction by parts optimization, modular compatibility, multidimensional regulation etc., as well as chassis evolution by DNA mutation and adaptive evolution. These strategies aims to break the bottleneck of carotenoid biosynthesis through improving the supply and utilization of precursor GGPP as well as relieving the stress from carotenoid accumulation on microbiological chassis. Based on the demand of employing unconventional chassis and expanding carotenoid kingdom, we also propose the development of the construction strategies for carotenoid microbiological cell factories towards the automation and intellectualization.

Key words: synthetic biology, cell factories, carotenoids, irrational design, pathway construction, chassis evolution

摘要:

类胡萝卜素具有丰富的颜色和强抗氧化活性,这赋予了该类产品较高的商业价值,加速了该类产品合成路径在异源底盘中的构建进程,使之成为发展非理性设计策略的有效研究对象。本文主要针对微生物合成类胡萝卜素细胞工厂构建中的瓶颈问题,从增强合成类胡萝卜素前体GGPP的供给利用以及缓解各类胡萝卜素产品对微生物底盘细胞的胁迫压力两个角度,系统综述非理性设计策略对元件优化、模块适配和多维调控等路径构建优化方法的丰富,以及对不同尺度的DNA变异和适应性进化等底盘进化手段的推动作用。并从扩展类胡萝卜素合成底盘物种的多样性和丰富非天然类胡萝卜素产品结构的可获得性的需求出发,对构建合成该类产品微生物细胞工厂自动化和智能化的发展趋势进行展望。

关键词: 合成生物学, 细胞工厂, 类胡萝卜素, 非理性设计, 路径构建, 底盘进化

CLC Number: 

京ICP备12046843号-2;京公网安备 11010102001994号
Copyright © Chemical Industry and Engineering Progress, All Rights Reserved.
E-mail: hgjz@cip.com.cn
Powered by Beijing Magtech Co. Ltd