Pectinase production with Rhizopus oryzae mycelium immobilized on cotton matrix and treatment of tobacco stem

doi:10.16085/j.issn.1000-6613.2016.05.34

Abstract

Abstract: The use of pectinases causes less environmental pollution in tobacco stem fiber processing for the production of lactic acid, etc. The effective way to improve pectinase production and reduce cost is to use a new combined fermentation mode and optimize its conditions. Mycelia of Rhizopus oryzaewere immobilized on the cotton matrix by immobilized cells technology developed in our laboratory. Conditions of pectinase production were optimized in culture medium of pectin or tobacco stem and the treatments of tobacco stem pectin were also optimized. The optimal fermentation conditions in pectin medium were rotary speed of 190r/min, liquid volume of 50mL/250mL, temperature of 30℃, pH value of 5, and spore concentration of 0.75×10⁶/mL. The optimal fermentation conditions in tobacco stem medium were, pH value of 4.6, spore concentration of 0.5×10⁶/mL, etc. Under the optimal treatment conditions of tobacco stem pectin, immobilized Rhizopus oryzaecontinuous processing method showed a better pectin degradation performance than the free pectinases processing method with the pectin degradation rate increased by 18.5%, up to 74.1%. Pectinase production with Rhizopus oryzae mycelium immobilized on the cotton matrix showed a higher pectinase production in pectin medium and better tobacco stem fiber degumming in tobacco stem fermentation.

Key words: Rhizopus oryzae, pectinase, immobilization, bio-degumming, tobacco stem

摘要： 利用果胶酶处理烟梗纤维生产乳酸等对环境友好,而应用新的发酵组合方式和优化发酵条件是提高产酶量,降低成本的有效途径。本文采用实验室研发的棉布支架固定化米根霉细胞的技术,优化了底物为果胶或烟梗的发酵产果胶酶的培养条件,还优化了处理烟梗果胶的条件。底物为果胶的最佳产酶条件为:转速190r/min,装液量50mL/250mL,发酵温度30℃,初始pH值5,初始孢子浓度0.75×10⁶个/mL。底物为烟梗的最佳产酶条件为:初始pH值4.6、初始孢子浓度0.5×10⁶个/mL等。在优化处理烟梗果胶的条件下,固定化米根霉产果胶酶连续法比游离的果胶酶法降解烟梗果胶的效果好,果胶降解率提高18.5%,达到74.1%。棉布支架固定化米根霉利用果胶发酵产果胶酶量较高,利用烟梗发酵脱胶效果较好。

关键词: 米根霉, 果胶酶, 固定化, 生物脱胶, 烟梗

CLC Number:

TQ920.6

HE Yuan, ZHENG Yuxi, PAN Jun, WANG Yuanliang . Pectinase production with Rhizopus oryzae mycelium immobilized on cotton matrix and treatment of tobacco stem[J]. Chemical Industry and Engineering Progree, 2016, 35(05): 1494-1501.

何源, 郑羽西, 潘君, 王远亮. 棉布支架固定化米根霉联产果胶酶及用于处理烟梗[J]. 化工进展, 2016, 35(05): 1494-1501.

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