Abstract: Eucommia bark is a rare traditional Chinese tonic herb,benefiting liver and kidney,strengthening tendons and bones,and preventing miscarriage.However,its effects are closely related to sex hormones,so the effects of three main iridoids of Eucommia(genipiin,geniposide,and geniposidic acid) on sex hormones' transformation were studied in this paper.KGN,a tumor ovarian granulosa cell line was chosen as the transformation system of hormone,pregnenolone as conversion substrate,progesterone,testosterone,and estradiol levels in the culture medium were measured respectively after interfered by the iridoids for 24 hours.Moreover,cell RNA was also collected to study the catalytic enzymes' expression levels(3β-HSD,CYP17A1,and 17β-HSD) by reverse transcription and real-time fluorescent quantitation method.The research results showed that 50 μmol/L genipin could significantly improve 3β-HSD,CYP17A1,and 17β-HSD expression levels,and significantly promote the synthesis of testosterone and estradiol further.

Key words: Eucommia, iridoid, sex hormone, aromatase, transformation

摘要： 杜仲是中国传统名贵滋补药材，有补益肝肾、强筋壮骨、固经安胎的功效，而这些功效与性激素水平息息相关，故本文研究了杜仲中3种主要的环烯醚萜类物质（京尼平、京尼平苷和京尼平苷酸）对性激素的转化作用。以卵巢颗粒细胞的肿瘤细胞系KGN作为激素的转化系统，孕烯醇酮为转化底物，分别测量环烯醚萜类物质干预24h后培养基中的孕酮、睾酮以及雌二醇的浓度，并提取细胞的RNA，以反转录实时荧光定量法来研究其催化酶3β-HSD、CYP17A1以及17β-HSD表达水平的影响。研究结果表明，50 μmol/L的京尼平能显著提升3β-HSD、CYP17A1以及17β-HSD的表达水平，从而显著促进睾酮和雌二醇的合成。

关键词: 杜仲, 环烯醚萜, 性激素, 芳香化酶, 转化