Abstract: A novel polyamine microsphere was prepared using inverse emulsion as the templet. In the W/O emulsion of aqueous solution of polyethylenimine(PEI)/ liquid paraffin，glutaraldehyde (GA)，the crosslinking agent，was added in a dropwise feeding mode. After the droplets coalescence，the crosslinking reaction happened at the interface of the emulsion droplets，and the polyamine microspheres were obtained. The microsphere is entirely spherical and shows a smooth outer surface. The size distribution of the microspheres ranges 0.37～4.29 ?m. The average particle size of the obtained miscrosheres is 1.44 ?m. The isoelectric point of the microsphere is 10.6. The analysis of TGA and DTG on the microspheres indicates that crosslinking significantly improves the decomposition temperature of PEI. The investigation results of protein adsorption demonstrates that the microspheres can selectively adsorb lipase from a mixture of lipase，cellulase，?-amylase，and pectinase. The maximum adsorption capacity of the microsphere for the lipase tested is 127.8 mg/g.

Key words: poly-amine microspheres, inverse emulsion, crosslinking polymerization, protein adsorption, lipase

摘要： 以反相乳液为模板，通过在聚乙烯亚胺（PEI）水溶液/液体石蜡的W/O乳状液中连续滴加戊二醛的方式，在液滴间聚并过程中，交联剂和PEI在油水界面发生交联反应，制备得到新型的聚胺基交联微球。所得微球表面光滑，球形对称，粒径分布范围0.37～4.29 ?m，平均粒径为 1.44 ?m。微球表面Zeta电位的等电点为10.6，热重分析结果表明交联反应提高了PEI的分解温度。吸附蛋白质的实验结果证明，微球能自脂肪酶、纤维素酶、?-淀粉酶、果胶酶的混合蛋白质溶液中选择性吸附脂肪酶，微球对所检测脂肪酶的最大吸附量为127.8 mg/g。

关键词: 聚胺基微球, 反相, 交联聚合, 蛋白质吸附, 脂肪酶