Abstract: To provide theoretical basis and experiment guidance for continuous biobutanol fermentation using cheap sugar substrate, the effects of dilution rate and temperature on continuous biobutanol fermentation by Clostridium saccharobutylicum DSM 13864 using glucose as substrate were investigated. The results showed that acid accumulation and cell density were enhanced at higher dilution rates, while solvent production was improved at lower dilution rates. At dilution rate of 0.05 h?1 and 37℃, total solvent concentration of 11.57 g/L (7.29 g/L butanol) and solvent productivity of 0.145 g/(L?h) were reached. The continuous fermentation process using temperature-shifting strategy demonstrated that a lower temperature was beneficial to solvent accumulation. When fermentation temperatures of the first two stages (Ⅰ & Ⅱ) and the later two stages (Ⅲ & Ⅳ) were controlled at 37 ℃ and 33 ℃ respectively, total solvent concentration of 13.69 g/L (8.36 g/L butanol) and solvent productivity of 0.171 g/(L?h) were achieved.

Key words: Clostridium saccharobutylicum, continuous fermentation, butanol, dilution rate, temperature

摘要： 为使用廉价糖质原料进行连续发酵生产丁醇提供理论依据和实验指导，以Clostridium saccharobutylicum DSM 13864为发酵菌种，考察了稀释率和温度等因素对以葡萄糖为原料的四级连续发酵生产丁醇的影响。结果表明：高稀释率有利于酸的积累和菌体的生长，低稀释率有利于溶剂的生产。当稀释率为0.05 h?1，4个发酵罐温度控制在37 ℃时，总溶剂产量为11.57 g/L，其中丁醇7.29 g/L，生产率为0.145 g/(L?h)。在0.05 h?1稀释率的条件下进行变温连续发酵证实低温有利于溶剂的积累，当一级和二级罐温度控制在37 ℃，三级和四级罐温度控制在33 ℃时总溶剂产量最高为13.69 g/L，其中丁醇为8.36 g/L，生产率为0.171 g/(L?h)。

关键词: Clostridium saccharobutylicum；连续发酵；丁醇；稀释率, 温度