Research progress of biological nitrogen removal by microbial fuel cell

doi:10.16085/j.issn.1000-6613.2016.04.040

Abstract

Abstract: Microbial fuel cell (MFC) is a new technology for wastewater treatment while generating electricity simultaneously. Applying MFC to nitrogen removal makes the system denitrification MFC or simultaneous nitrification and denitrification (SND) MFC. This paper firstly gave an introduction on the development of biological denitrification in MFC system. Then we analyzed factors influencing MFC nitrogen removal efficiency. The analysis was mainly focused on the MFC configuration (space configuration, electrode materials, separation materials), the operation conditions (nitrogen components concentrations, HRT, dissolved oxygen, carbon source and C/N ratio, temperature, pH, external resistance), as well as the gene expression and diversity of denitrifying bacteria in systems. Finally, suggestions were proposed as following:developing high economic and efficient electrode materials with strong electron transfer ability and ammonia oxidation catalytic function, optimizing the operation conditions of the MFC denitrification systems and analyzing nitrogen removal mechanism, and screening efficient denitrification bacteria through the analysis of microbial community structure in MFC denitrification systems.

Key words: microbial fuel cell (MFC), wastewater treatment, biological nitrogen removal, denitrification, catalysis, anaerobic

摘要： 微生物燃料电池(MFC)是一种新型污水处理技术,其在处理污水的同时能产生电能,引起众多研究者的关注.将MFC应用于含氮污水的处理中便形成了反硝化或同步硝化反硝化MFC系统.本文回顾了MFC生物脱氮的发展历程,并从MFC实验装置的设计构造(空间构型、电极材料、分隔材料)、影响因素(含氮污染物浓度、水力停留时间、溶解氧、碳源与碳氮比、温度、pH值、外电阻)和反硝化细菌的基因表达与多样性等3个方面进行了综述与分析,提出需要从以下方面进行MFC生物脱氮效能的强化:开发具有强电子传输能力和氨氧化催化功能的廉价高效电极材料,优化MFC脱氮的运行条件和探索不同环境下的脱氮机理,通过研究MFC阴极微生物种群构成筛选培育优势反硝化功能菌.

关键词: 微生物燃料电池, 污水处理, 生物脱氮, 反硝化, 催化, 厌氧

CLC Number:

X703.1

ZHAO Huimin, LI Xiaoling, ZHAO Jianqiang. Research progress of biological nitrogen removal by microbial fuel cell[J]. Chemical Industry and Engineering Progree, 2016, 35(04): 1216-1222.

赵慧敏, 李晓玲, 赵剑强. 微生物燃料电池在污水生物脱氮中的研究进展[J]. 化工进展, 2016, 35(04): 1216-1222.

References

[1] LOGANBE,HAMELERSB,ROZENDALR,et al.Microbial fuel cells methodology and technology[J]. Environmental Science Technology,2006,40(17):5181-5192.
[2] LIU H,RAMNARAYANAN R,LOGAN B E. Production of electricity during wastewater treatment using a single chamber microbial fuel cell[J].Environmental Science & Technology,2004,38(7):2281-2285.
[3] LIU X W,LI W W,YU H Q.Cathodic catalysts in bioelectrochemical systems for energy recovery from wastewater[J].Chemical Society Reviews,2014,43(22) :7718-7745.
[4] ZHANG F,HE Z.Integrated organic and nitrogen removal with electricity generation in a tubular dual-cathode microbial fuel cell[J].Process Biochemistry,2012,47(22):2146-2151.
[5] PARK D H,LAIVENIEKS M,GUETTLER M V,et al.Microbial utilization of electrically reduced neutral red as the sole electron donor for growth and metabolite production[J].Applied and Environmental Microbiology,1999,65 (7):2912-2917.
[6] HOLMES D E,BOND D R,O'NEIL R A,et al.Microbial communities associated with electrodes harvesting electricity from a variety of aquatic sediments[J].Microbial Ecology,2004,48(2):178-190.
[7] PUIG S,COMA M,DESLOOVER J,et al.Autotrophic denitrification in microbial fuel cells treating low ionic strength waters[J].Environmental Science & Technology,2012,46(4):2309-2315.
[8] 程建萍,孙秋阳,胡淑恒,等.两类阴极菌群微生物料电池降解硝酸盐研究[J].合肥工业大学学报(自然科学版)2014,37(10):1168-1172.
[9] THAUER R K,JUNGERMANN K,DECKER K.Energy conservation in chemotrophic anaerobic bacteria[J].Bacteriological Reviews,1977,41(1):100-180.
[10] VIRDIS B,READ S T,RABAEY K,et al.Biofilm stratification during simultaneous nitrification and denitrification(SND)at a biocathode[J].Bioresource Technology,2011,102(1):334-341.
[11] CLAUWAERT P,RABAEY K,AELTERMAN P,et al.Biological denitrification in microbial fuel cells[J].Environmental Science & Technology,2007,41(9),3354-3360.
[12] 王清萍,刘培文,翁秀兰,等.脱氮副球菌YF1微生物燃料电池生物阴极脱氮和产电[J].环境工程学报,2014,8(8):3277-3282.
[13] PUIG S, SERRAA M,VILAR-SANZ A,et al.Autotrophic nitrite removal in the cathode of microbial fuel cells[J].Bioresource Technology,2011,102(6):4462-4467.
[14] YAN H J,REGAN J M.Enhanced nitrogen removal in single-
chamber microbial fuel cells with increased gas diffusion areas[J].
Biotechnology & Bioengineering,2013,110(3):785-791.
[15] 章轶磊,崔康平,孙世群,等.单室不锈钢阳极微生物燃料电池强化反硝化的研究[J].广东化工,2012,39(2):4-7.
[16] 汪家权,夏雪兰,陈少华,等.两类微生物燃料电池治理硝酸盐废水的实验研究[J].环境科学学报,2011,31(2):254-259.
[17] SUKKASEM C,XU S T,PARK S,et al.Effect of nitrate on the performance of single chamber air cathode microbial fuel cells[J].Water Research,2008,42(19):4743-4750.
[18] XIE S,OUYANG K,LI L,et al.Researches of membranes as the separators for microbial fuel cells[J].Technology of Water Treatment,2011,37(8):15-18.
[19] ZHANG F,HE Z.Simultaneous nitrification and denitrification with electricity generation in dual-cathode microbial fuel cells[J].Chemistry Technology Biotechnology,2012,87(1):153-159.
[20] LEE K Y,RYU W S,CHO S I,et al.Comparative study on power generation of dual-cathode microbial fuel cell according to polarization methods[J].Water Research,2015,84:43-48.
[21] 印霞棐,刘维平.电极对微生物燃料电池同时处理有机废水和含铜重金属废水产电性能的影响[J].化工进展,2015,34(4):1152-1158.
[22] CHENG S A,LIU H,LOGAN B E.Power densities using different cathode catalysts(Pt and CoTMPP)and polymer binders (Nafione and PTFE)in single chamber microbial fuel cells[J].Environmental Science & Technology,2006,40(1):364-369.
[23] FANG C,MIN B,ANGELIDAKI I.Nitrate as anoxidant in the cathode chamber of a microbial fuel cell forboth power generation and nutrient removal purposes[J].Applied Biochemistry Biotechnology,2011, 164(4):464-474.
[24] 白立俊,王许云,何海波,等.M-N-C阴极催化剂的制备及其在微生物燃料电池中的应用[J].化工学报,2014,65(4):1267-1272.
[25] 谢倍珍,刘博杰,杨少强,等.生物阴极式碳纸隔膜微生物燃料池的反硝化和产电性能[J].环境工程学报,2014,8(6):2163-2168.
[26] JEON B Y,SEO H N,KANG S W,et al.Effect of electrochemical redox reaction on biochemical ammonium oxidation and chemical nitrite oxidation[J].Journal of Microbiology & Biotechnology,2010,20(3):485-493.
[27] ZUO Y,CHENG S A,LOGAN B E.Ion exchange membrane cathodes for scalable microbial fuel cells[J].Environmental Science & Technology, 2008,42(18):6967-6972.
[28] WATSON V J,SAITO T,HICKNER M A,et al.Polymercoatings as separator layers for microbial fuel cellcathodes[J].Power Sources,2011,196(6):3009-3014.
[29] LI Z Q,ZHANG Y,LEDUC P R,et al.Microbial electricity generation via microfluidic flow control[J].Biotechnology & Bioengineering,2011,108(9):2061-2069.
[30] SANTORO C,ARTYUSHKOVA K,GAJDA I,et al.Cathode materials for ceramic based microbial fuel cells (MFCs)[J].International Journal of Hydrogen Energy,2015,40(42):14706-14715.
[31] 梁鹏,张玲,黄霞,等.双筒型微生物燃料电池生物阴极反硝化研究[J].环境科学,2010,31(8):1932-1936.
[32] 张立成,于洋,程亚楠,等.硝酸盐质量浓度对微生物燃料电池产电及反硝化的影响[J].沈阳建筑大学学报(自然科学版),2013,29(6):1128-1132.
[33] 黄丽巧,易筱箔,韦朝海,等.阴极硝化耦合阳极反硝化实现微生物燃料电池技术脱氮[J].环境工程学报,2015,9(10):5118-5124.
[34] 张吉强,郑平,何崭飞,等.废水中硝氮和COD浓度对AD-MFC脱氮产电性能的影响[J].环境工程学报,2014,8(10):4508-4514.
[35] 华迪,崔康平,赵莉丽.微生物燃料电池原位修复地下水硝酸盐污染[J].广东化工,2014,41(1):93-94.
[36] 杨广伟,姜裙秋,王馄,等.生物产电人工湿地系统对处理生活污水的效能[J].浙江大学学报(工学版),2015,49(6):1186-1192.
[37] MOON H S,SHIN D Y,NAM K,et al.A long-term performance test on an autotrophic denitrification column for application as a permeable reactive barrier[J].Chemosphere,2008,73(5):723-728.
[38] 李金涛,张少辉.反硝化微生物燃料电池的基础研究[J].中国环境科学,2012,32(4):617-622.
[39] YU C P,LIANG Z H,DAS A,et al.Nitrogen removal from wastewater using membrane aerated microbial fuel cell techniques[J].Water Research,2011,45(3):1157-1164.
[40] ZHANG Y F,ANGELIDAKI I.Bioelectrode-based approach for enhancing nitrate and nitrite removal and electricity generation from eutrophic lakes[J].Water Research,2012,46(9):6445-6453.
[41] POUS N,PUIG S,BALAGUER M D,et al.Cathode potential and anode electron donor evaluation for a suitable treatment of nitrate-contaminated groundwater in bioelectrochemical systems[J].Chemical Engineering Journal,2014,263:151-159.
[42] 张立成,程亚楠,于洋,等.阴极室碳氮比对微生物燃料电池的影响[J].中国给水排水,2014,30(23):98-100.
[43] VIRDIS B,RABAEY K,YUAN Z G,et al.Electron fluxes in a microbial fuel cell performing carbon and nitrogen removal[J].Environmentalence & Technology,2009,43(13):5144-5149.
[44] LIU M,YUAN Y,ZHANG L X,et al.Bioelectricity generation by a Gram-positive Corynebacterium sp. Strain MFC03 under alkaline condition in microbial fuel cells[J].Bioresource Technology,2009,101(6):1807-1811.
[45] 赵煜,薄晓,马彦,等.不同温度下微生物燃料电池的运行特性[J].化工进展,2014,33(3):629-633.
[46] LI W Q,ZHANG S H,CHEN G,et al.Simultaneous electricity generation and pollutant removal in microbial fuel cell with denitrifying biocathode over nitrite[J].Applied Energy,2014,126:136-141.
[47] 车轩,罗国芝,谭洪新,等.脱氮硫杆菌的分离鉴定和反硝化特性研究[J].环境科学,2008,29(10):2931-2937.
[48] YOU S J,REN N Q,ZHAO Q L,et al.Improving phosphate buffer free cathode performance of microbial fuel cell based on biological nitrification[J]. Biosensors & Bioelectronics,2009,24(12):3698-3701.
[49] 贾璐维,赵剑强,胡博,等.MFC强化同步短程硝化反硝化工艺的启动[J].环境工程学报,2015,9(4):1831-1836.
[50] JANG J K,PHAM T H,CHANG I S,et al.Construction and operation of a novel mediator-and membrane-less microbial fuel cell[J].Process Biochemistry,2004,39(8):1007-1012.
[51] CUHEL J,SIMEK M,LAUGHLIN R,et al.Insights into the effect of soil pH on N₂O and N₂ emissions and denitrifier community size and activity[J].Applied & Environmental Microbiology,2010,76(6):1870-1878.
[52] WAKELIN S,NELSON P N,ARMOUR J D,et al.Bacterial community structure and denitrifier(nir-gene)abundance in soil water and groundwater beneath agricultural land in tropical North Queensland Australia[J].Soil Research,2011,49(1):65-76.
[53] KRISHNANI K K.Detection and diversity of nitrifying and denitrifying functional genes in coastal aquaculture[J].Aquaculture,2010,302(1):57-70.
[54] MORALES S E,COSART T,HOLBEN W E.Bacterial gene abundances as indicators of greenhouse gas emission in soils[J].Isme Journal,2010,4(6):799-808.
[55] RISGAARD-PETERSEN N,LANGEZAAL A M,INGVARDSEN S,et al.Evidence for complete denitrification in a benthic foraminifer[J].Nature,2006,443(7107):93-96.
[56] VILAR-SANZ A,PUIG S,GARCIA-LLEDO A,et al.Denitrifying bacterial communities affect current production and nitrous oxide accumulation in a microbial fuel cell[J].PLoS One,2013,8(5):991-994.
[57] GREGORY K B,BOND D R,LOVLEY D R.Graphite electrodes as electron donors for anaerobic respiration[J].Environmental Microbiology,2004,6(6):596-604.
[58] ZHU G C,ONODERA T,TANDUKAR M,et al.Simultaneous carbon removal,denitrification and power generation in a membrane-less microbialfuel cell[J].Bioresource Technology,2013,146(10):1-6.
[59] NGUYEN V K,HONG S,Park Y,et al.Autotrophic denitrification performance and bacterial community a biocathodes of bioelectro chemical systems with either abiotic or biotic anodes[J].Journal of Bioscience & Bioengineering,2014,119(2):180-187.
[60] KONDAVEETI S,LEE S H,Park H D,et al.Bacterial communities in a bioelectrochemical denitrification system:the effects of supplemental electron acceptors[J].Water Research,2014,51(6):25-36.
[61] SONG H L,ZHU Y,LI J.Electron transfer mechanisms characteristics and applications of biological cathode microbial fuel cells-A mini review[J].ArabianJournal of Chemistry,2015,7:1878-1886.
[62] CHEN G W,CHOI S J,LEE T H,et al.Application of biocathode in microbial fuel cells:cell performance and microbial community[J].Applied Microbiology & Biotechnology,2008,79(3):379-388.
[63] CHEN G W,CHOI S J,CHA J H,et al.Microbial community dynamics and electron transfer of a biocathode in microbial fuel cells[J].Korean Journal Chemical Engineering,2010,27(5):1513-1520.