Joint remediation of petroleum contaminated soil by urea peroxide with microorganism

doi:10.16085/j.issn.1000-6613.2021-2444

Abstract

Abstract:

In order to certificate the feasibility of joint remediation of chemical oxidation method with microorganism method for petroleum-contaminated soil. Using urea peroxide as the oxidant and Fe²⁺ as the activator, joint repair experiments and the orthogonal experiment design were used to investigate the conditions for urea peroxide (UHP) to remove petroleum pollutants in soil and the effect of UHP combined with microorganism on the remediation of petroleum contaminated soil. The optimal conditions for the chemical oxidation experiment were as followings: 4% mass fraction of UHP, UHP∶Fe²⁺ (molar ratio) = 30∶1 and water∶soil = 3∶2. Compared to the single microbial remediation method and single urea peroxide oxidation remediation method, the degradation rate of petroleum hydrocarbons with the urea peroxide combined with microbial methods for remediation of petroleum contaminated soils was increased by 35.45%—47.26% and 3.35%—15.16%, respectively. The sequence of urea peroxide and microorganism remediation was optimized. Compared to microbial remediation followed by urea peroxide remediation, the urea peroxide remediation followed by microbial remediation increased the degradation rate of petroleum hydrocarbons by 11.81% . This study shows that urea peroxide combined with microbial remediation is a good way to degrade petroleum in the soil.

Key words: urea peroxide, soil remediation, orthogonal experimental, activation, petroleum contamination

摘要：

为揭示化学氧化法与微生物法联合修复技术在石油污染土壤修复中应用可行性，本研究采用正交实验设计和联合修复实验，以过氧化尿素（UHP）为氧化剂，Fe²⁺为激活剂，研究了UHP去除石油烃的条件及其与微生物联合修复土壤石油污染物的效果。结果表明，利用UHP氧化土壤中石油烃的最佳条件为：UHP添加质量分数4%、UHP∶Fe²⁺（摩尔比）=30∶1及水∶土=3∶2。过氧化尿素氧化法与微生物法联合作用下，石油烃降解率比单一微生物修复法和过氧化尿素氧化修复法分别提高了35.45%~47.26%和3.35%~15.16%。过氧化尿素与微生物的修复顺序优化结果显示，过氧化尿素预氧化联合微生物修复比先进行微生物修复再进行过氧化尿素氧化对土壤中石油烃的降解率提高了11.81%。研究结果证明过氧化尿素联合微生物修复对降解土壤中石油烃污染的可行性。

关键词: 过氧化尿素, 土壤修复, 正交实验, 活化（作用）, 石油污染

CLC Number:

ZHENG Jin, HAN Ruirui, LI Dandan, WANG Xinyu, GAO Chunyang, DU Xianyuan, ZHANG XiaoFei, ZOU Dexun. Joint remediation of petroleum contaminated soil by urea peroxide with microorganism[J]. Chemical Industry and Engineering Progress, 2022, 41(9): 5085-5093.

郑瑾, 韩瑞瑞, 李丹丹, 王馨妤, 高春阳, 杜显元, 张晓飞, 邹德勋. 过氧化尿素与微生物联合修复石油污染土壤[J]. 化工进展, 2022, 41(9): 5085-5093.

Figures/Tables 11

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指标名称	值
石油烃/mg·kg^-1	44191
含水率/%	1.54
pH	8.17
有机碳/g·kg^-1	71.19
（总氮/总磷）/g·kg^-1	1.19/0.57
氨氮/mg·kg^-1	2.72
饱和烃/%	56.12
芳香烃/%	23.60
胶质/%	20.28

指标名称	值
石油烃/mg·kg^-1	44191
含水率/%	1.54
pH	8.17
有机碳/g·kg^-1	71.19
（总氮/总磷）/g·kg^-1	1.19/0.57
氨氮/mg·kg^-1	2.72
饱和烃/%	56.12
芳香烃/%	23.60
胶质/%	20.28

水平	UHP质量分数（A）/%	UHP∶Fe²⁺ （E）	水∶土（C）
1	1	10∶1	1∶2
2	2	30∶1	1∶1
3	4	50∶1	3∶2

水平	UHP质量分数（A）/%	UHP∶Fe²⁺ （E）	水∶土（C）
1	1	10∶1	1∶2
2	2	30∶1	1∶1
3	4	50∶1	3∶2

实验序号	水平组合	实验条件
实验序号	水平组合	UHP质量分数（A）/%	UHP∶Fe ²⁺（E）	水∶土（C）
1	A₁E₁C₁	1	10∶1	1∶2
2	A₁E₂C₂	1	30∶1	1∶1
3	A₁E₃C₃	1	50∶1	3∶2
4	A₂E₁C₂	2	10∶1	1∶1
5	A₂E₂C₃	2	30∶1	3∶2
6	A₂E₃C₁	2	50∶1	1∶2
7	A₃E₁C₃	4	10∶1	3∶2
8	A₃E₂C₁	4	30∶1	1∶2
9	A₃E₃C₂	4	50∶1	1∶1