Research on the lipid structure and compound differences of the extraction of Chlorella with different solvents

doi:10.16085/j.issn.1000-6613.2015.06.021

Chemical Industry and Engineering Progree ›› 2015, Vol. 34 ›› Issue (06): 1631-1635,1651.DOI: 10.16085/j.issn.1000-6613.2015.06.021

Previous Articles Next Articles

Research on the lipid structure and compound differences of the extraction of Chlorella with different solvents

DAI Xin¹, YANG Xuewei¹, CUI Yan¹, WANG Bo², ZHOU Lianning², CHENG Jiayang¹

1 Shenzen Engineering Laboratory for Algal Biofuel Technology Development and Application, School of Environment and Energy, Peking University-Shenzhen Graduate School, Shenzhen 518055, Guangdong, China;
2 IER Environmental Protection Engineering Technique Co., Ltd., Shenzhen 518000, Guangdong, China

Received:2014-11-02 Revised:2014-11-24 Online:2015-06-05 Published:2015-06-05

不同溶剂萃取小球藻油脂结构及组分差异

戴欣¹, 杨雪薇¹, 崔岩¹, 王波², 周连宁², 成家杨¹

1 北京大学深圳研究生院, 环境与能源学院, 深圳藻类新能源技术开发和应用工程实验室, 广东深圳 518055;
2 深圳市深港产学研环保工程技术股份有限公司, 广东深圳 518000

通讯作者: 成家杨,博士,教授,北京大学环境与能源学院院长,美国北卡罗莱那州立大学终身教授。E-mail:chengjy@pkusz.edu.cn。
作者简介:戴欣(1988—),男,硕士研究生。E-mail:buhuifei@pku.edu.cn。
基金资助:
国家海洋公益性行业科研专项(201305022)、中国博士后科学基金(2012M520106)、中国博士后科学基金第六批特别资助项目(2013T60025)及深圳市战略新兴产业发展专项(JCYJ20130329175919527, CXZZ20120618111150009)项目。

Abstract

Abstract: With the depleting supplies of fossil fuels, microalgae have attracted an increasing attention as a feedstock for biodiesel in recent years. However, a bottleneck issue with algal biodiesel manufacturing is the lack of efficient and highly selective extraction methods. According to literature, six organic solvents with different boiling points, polarity and oil-water partition coefficients were applied for lipid extraction from microalgae. The biomass structure and extractive compound were analyzed by FT-IR and GC-MS. The results showed that acetonitrile and n-heptane had the highest extraction yield with the value of 22.10% and 18.71%, respectively. The extraction yield was positively correlated to temperature. And the composition and structure of extraction products were the main factors that may influence extraction yield, while polarity and oil-water partition coefficient of the solvent did not affect extraction yield directly. The analysis of microalgae biomass structure and extraction product composition indicated that different solvents presented highly selectivity for the extraction of different compounds, like hydrocarbon, alcohol, and ester. Dichloromethane and acetone had the highest extraction yield on hydrocarbon, with up to 72.74% and 69.50%, n-heptane had the highest extraction yield on ester(80.46%), acetonitrile had the highest extraction yield on amine(58.52%), and ethyl acetate had the highest extraction on alcohol(24.65%).

Key words: microalgae, biodiesel, solvent extraction

摘要： 随着化石能源枯竭, 微藻生物柴油日益成为研究热点, 但对藻油的高效、高选择性萃取仍存在技术瓶颈。该文选择不同沸点、极性和油水分配系数的有机溶剂萃取微藻藻油, 并通过FTIR及GC-MS对微藻生物质结构及藻油组分差异进行明晰。结果表明:乙腈和正庚烷对藻油的提取率最高, 分别为22.10%和18.71%, 且与温度正相关, 但是溶剂的极性和油水分离系数对其萃取率无显著影响, 主要取决于所萃取产物的组分及结构;对微藻生物质结构及藻油组分研究表明不同溶剂对烃类、醇类、酯类等不同物质的萃取有较强的选择性;二氯甲烷和丙酮对烃类的萃取率高, 为72.74%和69.50%, 正庚烷对酯类的萃取率较高, 为80.46%, 乙腈对胺类的萃取率较高, 为58.52%, 乙酸乙酯对醇类的萃取率较高, 为24.65%。

关键词: 微藻, 生物柴油, 溶剂萃取

CLC Number:

Q819

DAI Xin, YANG Xuewei, CUI Yan, WANG Bo, ZHOU Lianning, CHENG Jiayang. Research on the lipid structure and compound differences of the extraction of Chlorella with different solvents[J]. Chemical Industry and Engineering Progree, 2015, 34(06): 1631-1635,1651.

戴欣, 杨雪薇, 崔岩, 王波, 周连宁, 成家杨. 不同溶剂萃取小球藻油脂结构及组分差异[J]. 化工进展, 2015, 34(06): 1631-1635,1651.

References

[1] Yang X, Dai X, Guo H, et al. Petrodiesel-like straight chain alkane and fatty alcohol production by the microalga Chlorella sorokiniana[J]. Bioresource Technology, 2013, 136:126-130.
[2] 陈国, 赵珺, 苏鹏飞, 等. 研究开发微藻产生物柴油研究进展[J]. 化工进展, 2011, 30(10):2186-2193.
[3] Vijayaraghavan K, Hemanathan K. Biodiesel production from freshwater algae[J]. Energy & Fuels, 2009, 23(11):5448-5453.
[4] Halim R, Gladman B, Danquah M K, et al. Oil extraction from microalgae for biodiesel production[J]. Bioresource Technology, 2011, 102(1):178-185.
[5] Rodríguez-Meizoso I, Jaime L, Santoyo S, et al. Subcritical water extraction and characterization of bioactive compounds from Haematococcus pluvialis microalga[J]. Journal of Pharmaceutical and Biomedical Analysis, 2010, 51(2):456-463.
[6] Li S, Han D, Row K H. Optimization of enzymatic extraction of polysaccharides from some marine algae by response surface methodology[J]. Korean Journal of Chemical Engineering, 2012, 29(5):650-656.
[7] 王雪青, 苗惠, 翟燕. 微藻细胞破碎方法的研究[J]. 天津科技大学学报, 2007, 22(1):21-25.
[8] 杨勋, 刘平怀, 郝宗娣, 等. 富油微藻Monoraphidium sp.的分离及其油脂提取工艺研究[J]. 安徽农业科学, 2011, 39(32):19988-19990.
[9] 孙协军, 王珍, 李秀霞. 盐藻油微波辅助提取工艺优化[J]. 食品科学, 2012, 33(10):87-91.
[10] Fajardo A R, Cerdán L E, Medina A R, et al. Lipid extraction from the microalga Phaeodactylum tricornutum[J]. European Journal of Lipid Science and Technology, 2007, 109(2):120-126.
[11] Bligh E G, Dyer W J. A rapid method of total lipid extraction and purification[J]. Canadian Journal of Biochemistry and Physiology, 1959, 37(8):911-917.
[12] Koberg M, Cohen M, Ben-Amotz A, et al. Bio-diesel production directly from the microalgae biomass of Nannochloropsis by microwave and ultrasound radiation[J]. Bioresource Technology, 2011, 102(5):4265-4269.
[13] Daroch M, Shao C, Liu Y, et al. Induction of lipids and resultant FAME profiles of microalgae from coastal waters of Pearl River Delta[J]. Bioresource Technology, 2013, 146:192-199.
[14] Wahlen B D, Willis R M, Seefeldt L C. Biodiesel production by simultaneous extraction and conversion of total lipids from microalgae, cyanobacteria, and wild mixed-cultures[J]. Bioresource Technology, 2011, 102(3):2724-2730.
[15] 卫生部. 化学品毒性鉴定技术规范[S]. 北京:卫生部卫生法制与监督司, 2005.
[16] 邓祥元, 高坤, 景吉, 等. 索氏法提取微藻油脂的工艺优化研究[J]. 安徽农业科学, 2012, 40(22):11370, 11385.
[17] Moreau R A, Powell M J, Singh V. Pressurized liquid extraction of polar and nonpolar lipids in corn and oats with hexane, methylene chloride, isopropanol, and ethanol[J]. Journal of the American Oil Chemists' Society, 2003, 80(11):1063-1067.
[18] Dean A P, Martin M C, Sigee D C. Resolution of codominant phytoplankton species in a eutrophic lake using synchrotron-based Fourier transform infrared spectroscopy[J]. Phycologia, 2007, 46(2):151-159.
[19] Heraud P, Wood B R, Tobin M J, et al. Mapping of nutrient-induced biochemical changes in living algal cells using synchrotron infrared microspectroscopy[J]. FEMS Microbiology Letters, 2005, 249(2):219-225.
[20] Benning L G, Phoenix V R, Yee N, et al. The dynamics of cyanobacterial silicification:An infrared micro-spectroscopic investigation[J]. Geochimica et Cosmochimica Acta, 2004, 68(4):743-757.
[21] Vardy S, Uwins P. Fourier transform infrared microspectroscopy as a tool to differentiate Nitzschia closterium and Nitzschia longissima[J]. Applied Spectroscopy, 2002, 56(12):1545-1548.
[22] Chiovitti A, Heraud P, Dugdale T M, et al. Divalent cations stabilize the aggregation of sulfated glycoproteins in the adhesive nanofibers of the biofouling diatom Toxarium undulatum[J]. Soft Matter., 2008, 4(4):811-820.
[23] Giordano M, Kansiz M, Heraud P, et al. Fourier transform infrared spectroscopy as a novel tool to investigate changes in intracellular macromolecular pools in the marine microalga Chaetoceros muellerii (Bacillariophyceae)[J]. Journal of Phycology, 2001, 37(2):271-279.

Research on the lipid structure and compound differences of the extraction of Chlorella with different solvents

不同溶剂萃取小球藻油脂结构及组分差异

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	QIAN Sitian, PENG Wenjun, ZHANG Xianming. Comparative analysis of forming cyclic oligomers via PET melt polycondensation and cyclodepolymerization [J]. Chemical Industry and Engineering Progress, 2023, 42(9): 4808-4816.
[2]	JIN Xin, LI Yushan, XIE Qingqing, WANG Mengyu, XIA Xingfan, YANG Chaohe. Progress on solketal synthesis catalyzed by porous materials [J]. Chemical Industry and Engineering Progress, 2023, 42(2): 731-743.
[3]	BAO Jin, SONG Yonghui, DONG Ping, LI Yifan, ZHU Rongyan, LIAO Long. Extraction and enrichment of iron ions in cyanide tailings electrolyte [J]. Chemical Industry and Engineering Progress, 2023, 42(1): 517-525.
[4]	QIN Zhenfang, LIAO Rihong, MA Weifang. Research progress on absorption-microalgae fixation of low concentration CO₂ and synchronous oil production in gas power plant [J]. Chemical Industry and Engineering Progress, 2023, 42(1): 94-106.
[5]	YE Yuxi, DING Xiaoxi, CHI Huarui, ZHU Kailun, LIU Yang, WANG Lingyun, GUO Qingjie. Hydrophobic deep eutectic solvent hydrogen bond interaction regulation and extraction performance of copper [J]. Chemical Industry and Engineering Progress, 2022, 41(S1): 397-406.
[6]	ZHAO Jianbing, YANG Dan, SHU Yuancao, ZHU Junbo, PU Shiping, SONG Xiaodan, LIU Shouqing, CHAI Xijuan, LI Xuemei. Preparation of Na₂CO₃/CF solid base and its catalytic transesterification of rapeseed oil [J]. Chemical Industry and Engineering Progress, 2022, 41(7): 3608-3614.
[7]	ZOU Pengcheng, JIN Guangyuan, LI Zhenfeng, SONG Chunfang, HAN Taibai, ZHU Yulian. Analysis of multi-physical field characteristics in a microwave reactor with a mode stirrer [J]. Chemical Industry and Engineering Progress, 2022, 41(5): 2301-2310.
[8]	NI Qing, LAI Jinbo, PENG Dongyue, GUAN Cuishi, LONG Jun. Progress in extraction separation of hydrocarbons by ionic liquids [J]. Chemical Industry and Engineering Progress, 2022, 41(2): 619-627.
[9]	MA Xin, WANG Shuang, LI Fashe, ZHANG Yishui, JIANG Shang. Simulation and experimental research on the atomization characteristics of waste oil biodiesel [J]. Chemical Industry and Engineering Progress, 2022, 41(2): 655-665.
[10]	JIA Wenlong, SONG Shuoshuo, LI Changjun, WU Xia, YANG Fan, ZHANG Yuanrui. Progress of oily sludge extraction by supercritical CO₂ [J]. Chemical Industry and Engineering Progress, 2022, 41(12): 6573-6585.
[11]	ZHU Changhui, ZHU Wenchao, LUO Jia, TIAN Baohe, SUN Jialin, ZOU Zhiyun. Recent advances in microwave-intensified transesterification for biodiesel preparation [J]. Chemical Industry and Engineering Progress, 2022, 41(10): 5145-5154.
[12]	JI Shulan, LI Xun, WANG Fei. Immobilization of Rhizopus oryzae onto loofah sponge as a whole-cell biocatalyst to preparation of biodiesel from Comus wilsoniana fruit oil [J]. Chemical Industry and Engineering Progress, 2022, 41(10): 5381-5389.
[13]	YUE Qianqian, GAO Lijing, XIAO Guomin, WEI Ruiping, LEI Yan. Process of the reactor and progress of biodiesel continuous production [J]. Chemical Industry and Engineering Progress, 2021, 40(S2): 81-88.
[14]	ZOU Shuai, LI Yuqin, MA Yiran, QI Zhenhua, JIA Quanwei. Diethanolamine strengthening CO₂ fixation and lipid accumulation in Coccomyxa subellipsoidea C-169 [J]. Chemical Industry and Engineering Progress, 2021, 40(9): 5222-5230.
[15]	LIU Shoujun, WANG Zhao, YAN Kang, YANG Song, SHANGGUAN Ju, DU Wenguang, CHANG Zhiwei, LIU Yuehua. Catalyst and hydrogen transport mechanism of catalytic hydrogenation of Shenfu long-flame coal [J]. Chemical Industry and Engineering Progress, 2021, 40(7): 3711-3718.