Abstract: Based on the good denitrification effectiveness of heterotrophic nitrification-aerobic denitrifier H1, this paper investigated the metabolic pathways and the heterotrophic nitrification characteristics of H1 under different conditions including dissolved oxygen concentration, wastewater composition and the metal ions. The results showed that when the dissolved oxygen concentration was 4.7 mg/L in the simulated NH4+ wastewater, the removal pathway of ammonia was NH4+—→ NH2OH —→N2O—→N2, which was the fast removal pathway. In the simulated NH4+ and NO2? wastewater, NH4+-N was removed by short - cut nitrification and denitrification, not showing priority denitrification by H1 in the whole process. In the simulated NH4+ and NO3? wastewater, NO3? induced hydroxylamine oxidase to produce NO2?-N, thus NH4+-N was removed by the nitrite levels of denitrification pathway. In the simulated NH4+ wastewater, 1 mmol/L Cu2+ and Fe2+ can significantly activate the activities of heterotrophic nitrification.

Key words: heterotrophic nitrification-aerobic denitrifier, heterotrophic nitrification property, dissolved oxygen, wastewater composition, metal ions

摘要： 在异养硝化-好氧反硝化菌H1良好的脱氮效果基础上，研究了在不同溶解氧浓度、废水成分和金属离子存在条件下时，H1的代谢途径及其异养硝化性能的变化。研究表明，溶解氧浓度在4.7 mg/L时，H1脱氮途径最佳；在NH4+模拟废水中，NH4+会通过NH4+—→NH2OH—→N2O—→N2的途径被快速去除；在NH4+和NO2?混合模拟废水中，没有显示出H1优先进行反硝化的现象，NH4+-N的降解是短程的硝化反硝化过程；在NH4+和NO3?混合模拟废水中，NO3?会诱导羟胺氧化酶产生NO2?-N，使得NH4+-N经过反硝化途径的亚硝酸盐水平被去除；在NH4+模拟废水中，1 mmol/L的Cu2+和Fe2+对异养硝化过程具有显著地激活作用。

关键词: 异养硝化-好氧反硝化菌, 异养硝化性能, 溶解氧, 废水成分, 金属离子