Rapid start-up performance of mainstream Anammox in a single-stage fixed-bed biofilm reactor

doi:10.16085/j.issn.1000-6613.2023-0541

Abstract

Abstract:

Mainstream anaerobic ammonium oxidation (Anammox) is a hotspot and difficulty issue for nitrogen removal from municipal wastewater. In this study, a single-stage fixed-bed biofilm reactor (SFBR) was used to treat simulated municipal wastewater. By switching from intermittent operation and controlling low-oxygen (DO: 0.4—0.7mg/L), mainstream Anammox was successfully started up after 65 days, and the system achieved removal efficiencies of ammonia nitrogen and total nitrogen up to 98.8% and 92.6%, respectively. At this moment, the biofilm structure was dense and appeared red. Microbial activity test showed that the activity of Anammox bacteria (SAA) was the highest among all the functional bacteria. High-throughput sequencing confirmed that the co-existence of Anammox genus (Candidatus Brocadia), partial denitrification genus (Thauera) and nitrifying genus (Nitrospira), but ammonia oxidizing bacteria was not detected. Further analysis of the amoA functional gene amplicon indicated that Nitrospira was actually complete ammonia oxidizing (Comammox) bacteria. Therefore, the system achieved nitrogen removal from municipal wastewater through the coupled pathway of complete nitrification/partial denitrification/Anammox within an oxygen-stratified biofilm. These study findings provided a new process idea for the rapid realization of mainstream Anammox.

Key words: biofilm, fixed-bed, reactor, mainstream Anammox, rapid start-up, nitrogen removal

摘要：

主流厌氧氨氧化（Anammox）是城市污水脱氮的热点和难点问题。本研究采用单级固定床生物膜反应器（SFBR）处理模拟城市污水，通过间歇运行切换和微氧（DO：0.4~0.7mg/L）控制，65天后成功启动Anammox。系统对氨氮和总氮去除率分别达98.8%和92.6%。此时生物膜结构致密，呈现红色。微生物活性测试发现，厌氧氨氧化菌活性在所有功能菌活性中最高。高通量测序证实厌氧氨氧化菌（Candidatus Brocadia）、短程反硝化菌（Thauera）和硝化菌（Nitrospira）共存，而未检测到氨氧化菌。amoA功能基因扩增子测序进一步分析表明，Nitrospira实际为全程氨氧化菌（Comammox）。因此，系统是在具有氧分层的生物膜内通过全程硝化/短程反硝化/厌氧氨氧化途径耦合实现城市污水脱氮。研究结果为主流Anammox快速实现提供了一种新的工艺思路。

关键词: 生物膜, 固定床, 反应器, 主流厌氧氨氧化, 快速启动, 脱氮

CLC Number:

X703

HAI Yan, ZHOU Xin, LI Yan. Rapid start-up performance of mainstream Anammox in a single-stage fixed-bed biofilm reactor[J]. Chemical Industry and Engineering Progress, 2024, 43(4): 2201-2209.

海岩, 周鑫, 李艳. 单级固定床生物膜反应器主流厌氧氨氧化快速启动性能[J]. 化工进展, 2024, 43(4): 2201-2209.

Figures/Tables 9

References 27

1	LEMOAL Morgane, Chantal GASCUEL-ODOUX, Alain MÉNESGUEN, et al. Eutrophication: A new wine in an old bottle?[J]. The Science of the Total Environment, 2019, 651(1): 1-11.
2	KHIN Than, ANNACHHATRE Ajit P. Novel microbial nitrogen removal processes[J]. Biotechnology Advances, 2004, 22(7): 519-532.
3	XIE Y D, ZHANG Q H, DZAKPASU M, et al. Towards the formulation of rural sewage discharge standards in China[J]. The Science of the Total Environment, 2021, 759: 143533.
4	ZHANG Liang, JIANG Ling, ZHANG Jiangtao, et al. Enhancing nitrogen removal through directly integrating Anammox into mainstream wastewater treatment: Advantageous, issues and future study[J]. Bioresource Technology, 2022, 362: 127827.
5	LOTTI Tommaso, BURZI Ottavia, SCAGLIONE Davide, et al. Two-stage granular sludge partial nitritation/Anammox process for the treatment of digestate from the anaerobic digestion of the organic fraction of municipal solid waste[J]. Waste Management, 2019, 100: 36-44.
6	KOWALSKI Maciej S, DEVLIN Tanner R, OLESZKIEWICZ Jan A. Start-up and long-term performance of Anammox moving bed biofilm reactor seeded with granular biomass[J]. Chemosphere, 2018, 200: 481-486.
7	YANG Shuai, YANG Fenglin. Nitrogen removal via short-cut simultaneous nitrification and denitrification in an intermittently aerated moving bed membrane bioreactor[J]. Journal of Hazardous Materials, 2011, 195: 318-323.
8	CHUNG Jinwook, Wookeun BAE, LEE Yong Woo, et al. Shortcut biological nitrogen removal in hybrid biofilm/suspended growth reactors[J]. Process Biochemistry, 2007, 42(3): 320-328.
9	ZHOU Xin, ZHANG Zeqian, ZHANG Xinai, et al. A novel single-stage process integrating simultaneous COD oxidation, partial nitritation-denitritation and Anammox (SCONDA) for treating ammonia-rich organic wastewater[J]. Bioresource Technology, 2018, 254: 50-55.
10	LI Xu, WANG Gonglei, CHEN Jiabo, et al. Deciphering the concurrence of Comammox, partial denitrification and Anammox in a single low-oxygen mainstream nitrogen removal reactor[J]. Chemosphere, 2022, 305: 135409.
11	陈加波, 周鑫, 李旭. 以活性污泥为接种污泥厌氧氨氧化工艺的快速启动及脱氮效能[J]. 化工进展, 2022, 41(7): 3900-3907.
	CHEN Jiabo, ZHOU Xin, LI Xu. Rapid start-up and nitrogen removal performance of Anammox process using activated sludge as an inoculation[J]. Chemical Industry and Engineering Progress, 2022, 41(7): 3900-3907.
12	MALOVANYY Andriy, TRELA Jozef, PLAZA Elzbieta. Mainstream wastewater treatment in integrated fixed film activated sludge (IFAS) reactor by partial nitritation/Anammox process[J]. Bioresource Technology, 2015, 198: 478-487.
13	WANG Chao, LIU Sitong, XU Xiaochen, et al. Achieving mainstream nitrogen removal through simultaneous partial nitrification, Anammox and denitrification process in an integrated fixed film activated sludge reactor[J]. Chemosphere, 2018, 203: 457-466.
14	MA Bin, QIAN Wenting, YUAN Chuansheng, et al. Achieving mainstream nitrogen removal through coupling Anammox with denitratation[J]. Environmental Science & Technology, 2017, 51(15): 8405-8413.
15	ZHANG Jianhua, ZHANG Qiong, LI Xiyao, et al. Rapid start-up of partial nitritation and simultaneously phosphorus removal (PNSPR) granular sludge reactor treating low-strength domestic sewage[J]. Bioresource Technology, 2017, 243: 660-666.
16	REHMAN Zahid Ur, FORTUNATO Luca, CHENG Tuoyuan, et al. Metagenomic analysis of sludge and early-stage biofilm communities of a submerged membrane bioreactor[J]. Science of the Total Environment, 2020, 701: 134682.
17	ZHAO Yunpeng, LIU Shufeng, JIANG Bo, et al. Genome-centered metagenomics analysis reveals the symbiotic organisms possessing ability to cross-feed with Anammox bacteria in Anammox consortia[J]. Environmental Science & Technology, 2018, 52(19): 11285-11296.
18	Beatriz FERNÁNDEZ-GÓMEZ, RICHTER Michael, Margarete SCHÜLER, et al. Ecology of marine Bacteroidetes: A comparative genomics approach[J]. The ISME Journal, 2013, 7(5): 1026-1037.
19	LODHA Tushar, NARVEKAR Simran, KARODI Prachi. Classification of uncultivated Anammox bacteria and Candidatus Uabimicrobium into new classes and provisional nomenclature as Candidatus Brocadiia classis nov. and Candidatus Uabimicrobiia classis nov. of the Phylum Planctomycetes and novel family Candidatus Scalinduaceae fam. nov to accommodate the genus Candidatus Scalindua[J]. Systematic and Applied Microbiology, 2021, 44(6): 126272.
20	LI Jianwei, PENG Yongzhen, ZHANG Liang, et al. Quantify the contribution of Anammox for enhanced nitrogen removal through metagenomic analysis and mass balance in an anoxic moving bed biofilm reactor[J]. Water Research, 2019, 160: 178-187.
21	SONG Tianwen, LI Shanshan, YIN Zichao, et al. Hydrolyzed polyacrylamide-containing wastewater treatment using ozone reactor-upflow anaerobic sludge blanket reactor-aerobic biofilm reactor multistage treatment system[J]. Environmental Pollution, 2021, 269: 116111.
22	DAIMS Holger, LEBEDEVA Elena V, PJEVAC Petra, et al. Complete nitrification by Nitrospira bacteria[J]. Nature, 2015, 528(7583): 504-509.
23	ANNAVAJHALA Medini K, KAPOOR Vikram, Jorge SANTO-DOMINGO, et al. Comammox functionality identified in diverse engineered biological wastewater treatment systems[J]. Environmental Science & Technology Letters, 2018, 5(2): 110-116.
24	SHAO Yung Hsien, WU Jer Horng. Comammox Nitrospira species dominate in an efficient partial nitrification-Anammox bioreactor for treating ammonium at low loadings[J]. Environmental Science & Technology, 2021, 55(3): 2087-2098.
25	COTTO Irmarie, DAI Zihan, HUO Linxuan, et al. Long solids retention times and attached growth phase favor prevalence of Comammox bacteria in nitrogen removal systems[J]. Water Research, 2020, 169: 115268.
26	GOTTSHALL Ekaterina Y, BRYSON Sam J, COGERT Kathryn I, et al. Sustained nitrogen loss in a symbiotic association of Comammox Nitrospira and Anammox bacteria[J]. Water Research, 2021, 202: 117426.
27	ROOTS Paul, WANG Yubo, ROSENTHAL Alex F, et al. Comammox Nitrospira are the dominant ammonia oxidizers in a mainstream low dissolved oxygen nitrification reactor[J]. Water Research, 2019, 157: 396-405.

阶段	时间/d	NH₄⁺-N /mg·L^-1	COD /mg·L^-1	C/N	DO/mg·L^-1	HRT /h	运行方式
Ⅰ	1~17	50	350	7	0.4~0.85	24	间歇
Ⅱ	18~32	50	150	3	0.4~0.65	36	间歇
Ⅲ	32~36	50	150	3	0.4~0.6	24	间歇
Ⅳ	37~65	50	150	3	0.4~0.7	24	连续

阶段	时间/d	NH₄⁺-N /mg·L^-1	COD /mg·L^-1	C/N	DO/mg·L^-1	HRT /h	运行方式
Ⅰ	1~17	50	350	7	0.4~0.85	24	间歇
Ⅱ	18~32	50	150	3	0.4~0.65	36	间歇
Ⅲ	32~36	50	150	3	0.4~0.6	24	间歇
Ⅳ	37~65	50	150	3	0.4~0.7	24	连续

反应器	启动时间/d	进水NH₄⁺-N/mg·L^-1	进水NO₂^--N/mg·L^-1	NRE/%	进水COD/mg·L^-1	HRT/h	参考文献
MBBR	89	35~50	—	36.3	45.5~65	—	[12]
IFAS	60	50	0	47.1	50	18	[13]
A/O 生物膜	115	60.5	—	80.0	157.3	—	[14]
SBBR	68	50.0	0	90.4	100	24	[10]
SBR	67	43.2~74.3	0~0.5	—	130.6~241.5	8	[15]
AnSBBR	40	21.6	28.4	90.1	0	24	[11]
SFBR	65	50	0	92.3	150	24	本研究

反应器	启动时间/d	进水NH₄⁺-N/mg·L^-1	进水NO₂^--N/mg·L^-1	NRE/%	进水COD/mg·L^-1	HRT/h	参考文献
MBBR	89	35~50	—	36.3	45.5~65	—	[12]
IFAS	60	50	0	47.1	50	18	[13]
A/O 生物膜	115	60.5	—	80.0	157.3	—	[14]
SBBR	68	50.0	0	90.4	100	24	[10]
SBR	67	43.2~74.3	0~0.5	—	130.6~241.5	8	[15]
AnSBBR	40	21.6	28.4	90.1	0	24	[11]
SFBR	65	50	0	92.3	150	24	本研究

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