Chemical Industry and Engineering Progress ›› 2023, Vol. 42 ›› Issue (12): 6498-6506.DOI: 10.16085/j.issn.1000-6613.2023-0046
• Biochemical and pharmaceutical engineering • Previous Articles
FANG Xiaoyu(), LU Diannan, LIU Zheng()
Received:
2023-01-10
Revised:
2023-02-20
Online:
2024-01-08
Published:
2023-12-25
Contact:
LIU Zheng
通讯作者:
刘铮
作者简介:
房晓宇(1997—),女,硕士研究生,研究方向为土壤生物修复。E-mail:fangxy20@mails.tsinghua.edu.cn。
CLC Number:
FANG Xiaoyu, LU Diannan, LIU Zheng. Recent advancements and applications of soil bioremediation techniques[J]. Chemical Industry and Engineering Progress, 2023, 42(12): 6498-6506.
房晓宇, 卢滇楠, 刘铮. 污染土壤生物修复技术的进展与工程应用现状[J]. 化工进展, 2023, 42(12): 6498-6506.
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URL: https://hgjz.cip.com.cn/EN/10.16085/j.issn.1000-6613.2023-0046
污染物类型 | 典型污染物 | 特点 | 生物修复技术 |
---|---|---|---|
无机污染物 | 重金属、微量元素、放射性核素、石棉等 | 不可降解,可溶,生物利用度低 | 生物钝化、植物挥发、生物浸出 |
有机污染物 | 农药、酚类化合物、药物和个人护理品等 | 可降解,可溶,有生物毒性,需筛选生物 | 微生物降解、生物通风、根际降解 |
石油烃、多环芳烃、硝基苯类、有机卤化物等 | 可降解,不可溶 | 微生物降解、生物通风、生物堆 | |
废弃生物质 | 农作物秸秆、林产废弃物、畜禽粪便等 | 可降解 | 微生物降解、生物堆、生物通风、根际降解 |
污染物类型 | 典型污染物 | 特点 | 生物修复技术 |
---|---|---|---|
无机污染物 | 重金属、微量元素、放射性核素、石棉等 | 不可降解,可溶,生物利用度低 | 生物钝化、植物挥发、生物浸出 |
有机污染物 | 农药、酚类化合物、药物和个人护理品等 | 可降解,可溶,有生物毒性,需筛选生物 | 微生物降解、生物通风、根际降解 |
石油烃、多环芳烃、硝基苯类、有机卤化物等 | 可降解,不可溶 | 微生物降解、生物通风、生物堆 | |
废弃生物质 | 农作物秸秆、林产废弃物、畜禽粪便等 | 可降解 | 微生物降解、生物堆、生物通风、根际降解 |
功能微生物 | 处理污染物 | 作用机制 | 参考文献 |
---|---|---|---|
HB-4 | 镉 | 表面分泌EPS吸附钝化重金属 | [ |
E. cloacae TU | 镉 | 表面化学基团钝化重金属 | [ |
硝基还原假单胞菌 | 镉 | 菌体表面生物矿化重金属 | [ |
R. sphaeroids SC01 | 铬 | 将硫酸盐转化为硫化物,形成硫化铬等复合物钝化金属 | [ |
Citrobacer | 镉 | 分解2-磷酸甘油,形成CdHPO4沉淀钝化金属 | [ |
P. fluorescens Q2-87 | 锌、镉 | 合成金属硫蛋白在细胞内部钝化重金属 | [ |
CRB5 | 铬 | 分泌铬酸还原酶降低重金属毒性 | [ |
R. metallidurans CH34 | 硒 | 周质或细胞质还原亚硒酸盐并在胞内沉积 | [ |
功能微生物 | 处理污染物 | 作用机制 | 参考文献 |
---|---|---|---|
HB-4 | 镉 | 表面分泌EPS吸附钝化重金属 | [ |
E. cloacae TU | 镉 | 表面化学基团钝化重金属 | [ |
硝基还原假单胞菌 | 镉 | 菌体表面生物矿化重金属 | [ |
R. sphaeroids SC01 | 铬 | 将硫酸盐转化为硫化物,形成硫化铬等复合物钝化金属 | [ |
Citrobacer | 镉 | 分解2-磷酸甘油,形成CdHPO4沉淀钝化金属 | [ |
P. fluorescens Q2-87 | 锌、镉 | 合成金属硫蛋白在细胞内部钝化重金属 | [ |
CRB5 | 铬 | 分泌铬酸还原酶降低重金属毒性 | [ |
R. metallidurans CH34 | 硒 | 周质或细胞质还原亚硒酸盐并在胞内沉积 | [ |
处理污染物 | 功能微生物 | 功能酶系 | 作用机制 | 参考文献 |
---|---|---|---|---|
石油烃 | 假单胞菌、阴沟肠杆菌、银汉小克霉菌 | 单/双加氧酶、脱氢酶 | 单/双加氧酶和脱氢酶通过单末端氧化、双末端氧化、次末端氧化、ω-氧化和β-氧化降解链烷烃,不同加氧酶将环烷烃依次氧化为环醇、环酮,开环后再进入三羧酸循环 | [ |
多环芳烃 | 假单胞菌、芽孢杆菌、白腐真菌 | 单/双加氧酶、细胞色素P450酶、木质素降解酶系 | 单/双加氧酶和细胞色素P450酶将多环芳烃氧化为二氢二醇化合物、木质素降解酶系将多环芳烃氧化为醌类物质 | [ |
炸药(硝基苯类化合物) | 假单胞菌、红平红球菌、黄孢原毛平革菌 | 硝基还原酶、加氧酶 | 硝基还原酶将苯环上硝基还原为氨基或对苯环加氢还原,直接脱去硝基,释放亚硝酸根离子 | [ |
多氯联苯 | 产碱杆菌、无色杆菌、红球菌 | 联苯双加氧酶、脱卤酶 | 联苯双加氧酶氧化多氯联苯的苯环上的2,3位生成顺二氢二醇产物,脱卤酶在厌氧条件下对多氯联苯进行还原脱氯 | [ |
有机氯农药 | 克雷白氏杆菌菌、芽孢杆菌、白腐真菌 | 脱卤酶、脱氯化氢酶、谷胱甘肽转移酶 | 脱卤酶结合有机氯农药催化C—Cl键水解,脱氯化氢酶从底物上脱去氯化氢并生成碳碳双键,谷胱甘肽转移酶催化有机氯农药与谷胱甘肽结合脱氯 | [ |
有机磷农药 | 芽孢杆菌、黄杆菌曲霉、放线菌 | 有机磷水解酶、细胞色素P450酶、糖基转移酶 | 有机磷水解酶裂解P—O键、P—C键、P—S键,细胞色素P450酶对有机磷农药进行羟基化或N-脱烷基化反应,糖基转移酶将有机物与糖氨基酸或谷胱甘肽结合 | [ |
处理污染物 | 功能微生物 | 功能酶系 | 作用机制 | 参考文献 |
---|---|---|---|---|
石油烃 | 假单胞菌、阴沟肠杆菌、银汉小克霉菌 | 单/双加氧酶、脱氢酶 | 单/双加氧酶和脱氢酶通过单末端氧化、双末端氧化、次末端氧化、ω-氧化和β-氧化降解链烷烃,不同加氧酶将环烷烃依次氧化为环醇、环酮,开环后再进入三羧酸循环 | [ |
多环芳烃 | 假单胞菌、芽孢杆菌、白腐真菌 | 单/双加氧酶、细胞色素P450酶、木质素降解酶系 | 单/双加氧酶和细胞色素P450酶将多环芳烃氧化为二氢二醇化合物、木质素降解酶系将多环芳烃氧化为醌类物质 | [ |
炸药(硝基苯类化合物) | 假单胞菌、红平红球菌、黄孢原毛平革菌 | 硝基还原酶、加氧酶 | 硝基还原酶将苯环上硝基还原为氨基或对苯环加氢还原,直接脱去硝基,释放亚硝酸根离子 | [ |
多氯联苯 | 产碱杆菌、无色杆菌、红球菌 | 联苯双加氧酶、脱卤酶 | 联苯双加氧酶氧化多氯联苯的苯环上的2,3位生成顺二氢二醇产物,脱卤酶在厌氧条件下对多氯联苯进行还原脱氯 | [ |
有机氯农药 | 克雷白氏杆菌菌、芽孢杆菌、白腐真菌 | 脱卤酶、脱氯化氢酶、谷胱甘肽转移酶 | 脱卤酶结合有机氯农药催化C—Cl键水解,脱氯化氢酶从底物上脱去氯化氢并生成碳碳双键,谷胱甘肽转移酶催化有机氯农药与谷胱甘肽结合脱氯 | [ |
有机磷农药 | 芽孢杆菌、黄杆菌曲霉、放线菌 | 有机磷水解酶、细胞色素P450酶、糖基转移酶 | 有机磷水解酶裂解P—O键、P—C键、P—S键,细胞色素P450酶对有机磷农药进行羟基化或N-脱烷基化反应,糖基转移酶将有机物与糖氨基酸或谷胱甘肽结合 | [ |
技术 | 污染物 | 作用机制 | 应用案例 |
---|---|---|---|
曝气-生物联合修复 | 有机卤化物、挥发性有机物 | 增强包气带、含水层土壤的生物通风,强化好氧生物降解 | [ |
表面活性剂-生物联合修复 | 石油烃 | 提高污染物的生物可利用度,改变微生物表面的疏水性 | [ |
生物炭-生物联合修复 | 重金属、石油烃 | 富集污染物,中和土壤pH,提供营养支持微生物生长 | [ |
秸秆-生物联合修复 | 重金属、石油烃 | 提高土壤通透性,吸附石油污染物,转化后的腐殖质钝化重金属离子 | [ |
化学氧化-生物联合修复 | 石油烃、多环芳烃 | 化学预氧化大分子有机污染物,产生电子受体和生物可利用的有机小分子 | [ |
电动-生物联合修复 | 石油烃、多环芳烃 | 电场强化污染物、电子受体、营养物质和微生物的迁移,增强传质 | [ |
技术 | 污染物 | 作用机制 | 应用案例 |
---|---|---|---|
曝气-生物联合修复 | 有机卤化物、挥发性有机物 | 增强包气带、含水层土壤的生物通风,强化好氧生物降解 | [ |
表面活性剂-生物联合修复 | 石油烃 | 提高污染物的生物可利用度,改变微生物表面的疏水性 | [ |
生物炭-生物联合修复 | 重金属、石油烃 | 富集污染物,中和土壤pH,提供营养支持微生物生长 | [ |
秸秆-生物联合修复 | 重金属、石油烃 | 提高土壤通透性,吸附石油污染物,转化后的腐殖质钝化重金属离子 | [ |
化学氧化-生物联合修复 | 石油烃、多环芳烃 | 化学预氧化大分子有机污染物,产生电子受体和生物可利用的有机小分子 | [ |
电动-生物联合修复 | 石油烃、多环芳烃 | 电场强化污染物、电子受体、营养物质和微生物的迁移,增强传质 | [ |
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