Advances in microbial remediation of soils polluted by polycyclic aromatic hydrocarbons

doi:10.16085/j.issn.1000-6613.2021-1482

Chemical Industry and Engineering Progress ›› 2022, Vol. 41 ›› Issue (6): 3249-3262.DOI: 10.16085/j.issn.1000-6613.2021-1482

• Resources and environmental engineering • Previous Articles Next Articles

Advances in microbial remediation of soils polluted by polycyclic aromatic hydrocarbons

LYU Ying¹^,²^,³^,⁴(), HU Xuewu¹^,²^,³^,⁴, CHEN Susu¹^,³^,⁴, LIU Xingyu¹^,³^,⁵(), CHEN Bowei¹^,³^,⁵, ZHANG Mingjiang¹^,³^,⁵

^1.National Engineering Laboratory of Biohydrometallurgy, GRINM Group Corporation Limited, Beijing 101407, China
^2.School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, Beijing 100083, China
^3.GRINM Resources and Environment Tech. Co. , Ltd. , Beijing 101407, China
^4.General Research Institute for Nonferrous Metals, Beijing 100088, China
^5.GRIMAT Engineering Institute Co. , Ltd. , Beijing 101407, China

Received:2020-07-13 Revised:2020-07-29 Online:2022-06-21 Published:2022-06-10
Contact: LIU Xingyu

多环芳烃污染土壤的微生物修复技术研究进展

吕莹¹^,²^,³^,⁴(), 胡学武¹^,²^,³^,⁴, 陈素素¹^,³^,⁴, 刘兴宇¹^,³^,⁵(), 陈勃伟¹^,³^,⁵, 张明江¹^,³^,⁵

^1.有研科技集团有限公司生物冶金国家工程实验室，北京 101407
^2.北京科技大学冶金与生态工程学院，北京 100083
^3.有研资源环境技术研究院(北京)有限公司，北京 101407
^4.北京有色金属研究总院，北京 100088
^5.有研工程技术研究院有限公司，北京 101407

通讯作者: 刘兴宇
作者简介:吕莹（1994—），女，博士研究生, 研究方向为环境污染微生物修复。E-mail：lvying940904@163.com。
基金资助:
国家自然科学基金(51974279);科军评[2018]159号;国家重点研发计划(2018YFC18018);广西科学研究与技术开发计划(GuikeAB16380287)

Abstract

Abstract:

The pollution of polycyclic aromatic hydrocarbons (PAHs) in soil has become a serious environmental problem. Therefore, it is necessary to develop low cost and efficient technology of microbial remediation. Based on the environmental pollution characteristics of PAHs in soil, and combined with the research progress of removing PAHs from soil by microbial remediation technology in recent years, this paper analyzes the existing challenges and solutions of the engineering application. Then the mechanism of interaction between microorganisms and PAHs is introduced. It is pointed out that bacteria degrade PAHs mainly by dioxygenase, fungi degrade PAHs by monooxygenase, while algae degrade low-cyclic PAHs mainly by monooxygenase system, and high-cyclic PAHs mainly by dioxygenase system. Finally, the main research directions of remediation technology for PAHs contaminated soil in the future are proposed, including the establishment of screening system for efficient degrading bacteria, the construction of mixed microorganisms and genetically engineering bacteria, and the strengthening of the reaction process and metabonomics research, in order to provide guidance for the industrialization development and large-scale application of soil remediation technology in China.

Key words: polycyclic aromatic hydrocarbons, soil contamination, microbial remediation, degradable mechanism

摘要：

土壤中多环芳烃（PAHs）污染已成为一个严重的环境问题。因此，有必要开展低成本、高效的微生物修复技术研究。本文从土壤中PAHs的环境污染特征出发，结合近年来利用微生物修复技术去除土壤中PAHs的研究进展，剖析该技术工程应用存在的挑战及其解决策略。并对微生物与PAHs之间的作用机制进行介绍，指出细菌降解PAHs主要通过双加氧酶的作用，真菌降解PAHs利用的是单加氧酶，而藻类降解低环PAHs主要采用单加氧酶系统进行代谢，降解高环PAHs则主要采用双加氧酶系统进行代谢。最后提出了未来PAHs污染土壤修复技术的主要研究方向，包括建立高效降解菌筛选体系、构建混合菌群及基因工程菌、加强作用过程及代谢组学研究等方面，以期为我国土壤修复技术的产业化发展和大规模应用提供指导。

关键词: 多环芳烃, 土壤污染, 微生物修复, 降解机理

CLC Number:

LYU Ying, HU Xuewu, CHEN Susu, LIU Xingyu, CHEN Bowei, ZHANG Mingjiang. Advances in microbial remediation of soils polluted by polycyclic aromatic hydrocarbons[J]. Chemical Industry and Engineering Progress, 2022, 41(6): 3249-3262.

吕莹, 胡学武, 陈素素, 刘兴宇, 陈勃伟, 张明江. 多环芳烃污染土壤的微生物修复技术研究进展[J]. 化工进展, 2022, 41(6): 3249-3262.

Figures/Tables 7

References 115

1	SAZYKIN I S, MINKINA T M, KHMELEVTSOVA L E, et al. Polycyclic aromatic hydrocarbons, antibiotic resistance genes, toxicity in the exposed to anthropogenic pressure soils of the Southern Russia[J]. Environmental Research, 2021, 194: 110715.
2	YAN J, WANG L, FU P P, et al. Photomutagenicity of 16 polycyclic aromatic hydrocarbons from the US EPA priority pollutant list[J]. Mutat. Res., 2004, 557(1): 99-108.
3	蔡文良, 罗固源, 许晓毅, 等. 嘉陵江重庆段表层水体多环芳烃的污染特征[J]. 环境科学, 2012, 33(7): 2341-2346.
	CAI Wenliang, LUO Guyuan, XU Xiaoyi, et al. Contamination characteristics of polycyclic aromatic hydrocarbons(PAHs) in surface water from Jialing river in Chongqing[J]. Environmental Science, 2012, 33(7): 2341-2346.
4	HARMSEN J, RIETRA R P J J. 25 Years monitoring of PAHs and petroleum hydrocarbons biodegradation in soil[J]. Chemosphere, 2018, 207: 229-238.
5	LU C, HONG Y, LIU J, et al. A PAH-degrading bacterial community enriched with contaminated agricultural soil and its utility for microbial bioremediation[J]. Environmental Pollution, 2019, 251: 773-782.
6	CHEN M, XU P, ZENG G M, et al. Bioremediation of soils contaminated with polycyclic aromatic hydrocarbons, petroleum, pesticides, chlorophenols and heavy metals by composting: applications, microbes and future research needs[J]. Biotechnology Advances, 2015, 33(6): 745-755.
7	曾军, 吴宇澄, 林先贵. 多环芳烃污染土壤微生物修复研究进展[J]. 微生物学报, 2020, 60(12): 2804-2815.
	ZENG Jun, WU Chengwu, LIN Xiangui. Advances in microbial remediation of soils polluted by polycyclic aromatic hydrocarbons[J]. Acta Microbiologica Sinica, 2020, 60(12): 2804-2815.
8	KRONENBERG M, TRABLY E, BERNET N, et al. Biodegradation of polycyclic aromatic hydrocarbons: using microbial bioelectrochemical systems to overcome an impasse[J]. Environmental Pollution, 2017, 231: 509-523.
9	韩玲, 高照琴, 白军红, 等. 城市化背景下珠江三角洲典型湿地土壤多环芳烃(PAHs)的含量、来源与污染风险评价[J]. 农业环境科学学报, 2019, 38(3): 609-617.
	HAN Ling, GAO Zhaoqin, BAI Junhong, et al. PAHs in surface wetland soils of the Pearl River Delta affected by urbanization: levels, sources, and toxic risks[J]. Journal of Agro-Environment Science, 2019, 38(3): 609-617.
10	YANG J, SUN P, ZHANG X, et al. Source apportionment of PAHs in roadside agricultural soils of a megacity using positive matrix factorization receptor model and compound-specific carbon isotope analysis[J]. Journal of Hazardous Materials, 2021, 403: 123592.
11	WILD S R, JONES K C. Polynuclear aromatic hydrocarbons in the United Kingdom environment: a preliminary source inventory and budget[J]. Environmental Pollution, 1995, 88(1): 91-108.
12	MARCO-URREA E, GARCÍA-ROMERA I, ARANDA E. Potential of non-ligninolytic fungi in bioremediation of chlorinated and polycyclic aromatic hydrocarbons[J]. New Biotechnology, 2015, 32(6): 620-628.
13	KUPPUSAMY S, THAVAMANI P, VENKATESWARLU K, et al. Remediation approaches for polycyclic aromatic hydrocarbons (PAHs) contaminated soils: technological constraints, emerging trends and future directions[J]. Chemosphere, 2017, 168: 944-968.
14	WANG Y, NIE M Q, DIWU Z J, et al. Toxicity evaluation of the metabolites derived from the degradation of phenanthrene by one of a soil ubiquitous PAHs-degrading strain Rhodococcus qingshengii FF[J]. Journal of Hazardous Materials, 2021, 415: 125657.
15	申国兰, 李利, 陈莎. 微生物降解石油源多环芳香烃的研究进展[J]. 土壤, 2018, 50(1): 16-27.
	SHEN Guolan, LI Li, CHEN Sha, et al. Microbial degradation of polycyclic aromatic hydrocarbons from crude oils: a review[J]. Soils, 2018, 50(1): 16-27.
16	WU P, WANG Y S. Fluorene degradation by Rhodococcus sp. A2-3 isolated from hydrocarbon contaminated sediment of the Pearl River estuary, China[J]. Ecotoxicology, 2021, 30(5): 929-935.
17	WAIGI M G, KANG F X, GOIKAVI C, et al. Phenanthrene biodegradation by sphingomonads and its application in the contaminated soils and sediments: a review[J]. International Biodeterioration & Biodegradation, 2015, 104: 333-349.
18	SONG X H, XU Y, LI G M, et al. Isolation, characterization of Rhodococcus sp. P14 capable of degrading high-molecular-weight polycyclic aromatic hydrocarbons and aliphatic hydrocarbons[J]. Marine Pollution Bulletin, 2011, 62(10): 2122-2128.
19	JIANG R H, LI Y, WANG H Q, et al. A study on the degradation efficiency of fluoranthene and the transmembrane protein mechanism of Rhodococcus sp. BAP-1 based on iTRAQ[J]. Science of the Total Environment, 2020, 737: 140208.
20	KATHI S, KHAN A B. Enrichment, isolation and identification of polycyclic aromatic hydrocarbon degrading Rhodococcus ruber from sediments[J]. International Journal of Scientific and Research Publications, 2013, 3(2): 1-7.
21	MIAO L L, QU J, LIU Z P. Hydroxylation at multiple positions initiated the biodegradation of indeno[1,2,3-cd]p _y rene in Rhodococcus aetherivorans IcdP1[J]. Frontiers in Microbiology, 2020, 11: 568381.
22	TIRKEY S R, RAM S, MISHRA S. Naphthalene degradation studies using Pseudomonas sp. strain SA3 from Alang-Sosiya ship breaking yard, Gujarat[J]. Heliyon, 2021, 7(3): e06334.
23	SWATI, KUMARI M, GHOSH P, et al. Evaluation of a biosurfactant producing bacterial strain Pseudomonas sp. ISTPY₂ for efficient pyrene degradation and landfill soil bioremediation through soil microcosm and proteomic studies[J]. Bioresource Technology Reports, 2020, 12: 100607.
24	LI J, CHEN W X, ZHOU W, et al. Synergistic degradation of pyrene by Pseudomonas aeruginosa PA06 and Achromobacter sp. AC15 with sodium citrate as the co-metabolic carbon source[J]. Ecotoxicology, 2020: 1-12.
25	MAWAD A M M, ABDEL-MAGEED W S, HESHAM A E. Quantification of naphthalene dioxygenase (NahAC) and catechol dioxygenase (C23O) catabolic genes produced by phenanthrene-degrading Pseudomonas fluorescens AH-40[J]. Current Genomics, 2020, 21(2): 111-118.
26	NWINYI O C, AJAYI O O, AMUND O O. Degradation of polynuclear aromatic hydrocarbons by two strains of Pseudomonas [J]. Brazilian Journal of Microbiology, 2016, 47(3): 551-562.
27	KAFILZADEH F, POUR F H. Degradation of naphthalene, phenanthrene and pyrene by Pseudomonas sp. and Corynebacterium sp. in the landfills[J]. International Journal of Biosciences, 2012, 2(9): 77-84.
28	MOHD-KAMIL N A F, ALIAS S, OTHMAN N, et al. Degradation of phenanthrene by corynebacterium urealyticum in liquid culture and sand slurry[J]. Malaysian Journal of Soil Science, 2013, 17: 111-126.
29	GURAV R, LYU H H, MA J L, et al. Degradation of n-alkanes and PAHs from the heavy crude oil using salt-tolerant bacterial consortia and analysis of their catabolic genes[J]. Environmental Science and Pollution Research, 2017, 24(12): 11392-11403.
30	KAWO A H, BACHA H Y. Crude oil degradation by Bacillus and Micrococcus species isolated from soil compost in Kano, Nigeria[J]. Bayero Journal of Pure and Applied Sciences, 2016, 9(1): 108.
31	A. K H, C. P K. Degradation of low molecular weight polycyclic aromatic hydrocarbons by microorganisms isolated from contaminated soil[J]. International Journal OF Environmental Sciences, 2016, 6: 472-482.
32	DIB J R, ANGELOV A, LIEBL W, et al. Complete genome sequence of the linear plasmid pJD12 hosted by Micrococcus sp. D12, isolated from a high-altitude volcanic lake in Argentina[J]. Genome Announcements, 2015, 3(3): e00675.
33	SUN L, ZHU G H, LIAO X Y, et al. Interactions between Pteris vittata L. genotypes and a polycyclic aromatic hydrocarbon (PAH)-degrading bacterium (Alcaligenes sp.) in arsenic uptake and PAH-dissipation[J]. Environmental Pollution, 2017, 230: 862-870.
34	YALAOUI-GUELLAL D, FELLA-TEMZI S, DJAFRI-DIB S, et al. The petroleum-degrading bacteria Alcaligenes aquatilis strain YGD 2906 as a potential source of lipopeptide biosurfactant[J]. Fuel, 2021, 285: 119112.
35	SINGHA L P, PANDEY P. Rhizobacterial community of Jatropha curcas associated with pyrene biodegradation by consortium of PAH-degrading bacteria[J]. Applied Soil Ecology, 2020, 155: 103685.
36	MUANGCHINDA C, PANSRI R, WONGWONGSEE W, et al. Assessment of polycyclic aromatic hydrocarbon biodegradation potential in mangrove sediment from Don Hoi Lot, Samut Songkram Province, Thailand[J]. Journal of Applied Microbiology, 2013, 114(5): 1311-1324.
37	JOHN R C, ESSIEN J P, AKPAN S B, et al. Polycyclic aromatic hydrocarbon-degrading bacteria from aviation fuel spill site at ibeno, Nigeria[J]. Bulletin of Environmental Contamination and Toxicology, 2012, 88(6): 1014-1019.
38	SUN S S, WANG H Z, CHEN Y Z, et al. Salicylate and phthalate pathways contributed differently on phenanthrene and pyrene degradations in Mycobacterium sp. WY10[J]. Journal of Hazardous Materials, 2019, 364: 509-518.
39	GUO C L, DANG Z, WONG Y, et al. Biodegradation ability and dioxgenase genes of PAH-degrading Sphingomonas and Mycobacterium strains isolated from mangrove sediments[J]. International Biodeterioration & Biodegradation, 2010, 64(6): 419-426.
40	OHTSUBO Y, NONOYAMA S, OGAWA N, et al. Complete genome sequence of Burkholderia caribensis Bcrs1W (NBRC110739), a strain co-residing with phenanthrene degrader Mycobacterium sp. EPa₄₅ [J]. Journal of Biotechnology, 2016, 228: 67-68.
41	SEO J S, KEUM Y S, LI Q X. Mycobacterium aromativorans JS19b1T degrades phenanthrene through C-1, 2, C-3, 4 and C-9, 10 dioxygenation pathways[J]. International Biodeterioration & Biodegradation, 2012, 70: 96-103.
42	YUAN K, XIE X Q, WANG X W, et al. Transcriptional response of Mycobacterium sp. strain A1-PYR to multiple polycyclic aromatic hydrocarbon contaminations[J]. Environmental Pollution, 2018, 243: 824-832.
43	ZHONG J N, LUO L J, CHEN B W, et al. Degradation pathways of 1-methylphenanthrene in bacterial Sphingobium sp. MP9-4 isolated from petroleum-contaminated soil[J]. Marine Pollution Bulletin, 2017, 114(2): 926-933.
44	FU B, LI Q X, XU T, et al. Sphingobium sp. FB₃ degrades a mixture of polycyclic aromatic hydrocarbons[J]. International Biodeterioration & Biodegradation, 2014, 87: 44-51.
45	CUNLIFFE M, KERTESZ M A. Effect of Sphingobium yanoikuyae B1 inoculation on bacterial community dynamics and polycyclic aromatic hydrocarbon degradation in aged and freshly PAH-contaminated soils[J]. Environmental Pollution, 2006, 144(1): 228-237.
46	PINYAKONG O, HABE H, KOUZUMA A, et al. Isolation and characterization of genes encoding polycyclic aromatic hydrocarbon dioxygenase from acenaphthene and acenaphthylene degrading Sphingobium sp. strain A4[J]. FEMS Microbiology Letters, 2004, 238(2): 297-305.
47	MAEDA A H, NISHI S, HATADA Y, et al. Biotransformation of the high-molecular weight polycyclic aromatic hydrocarbon (PAH) benzo[k]fluoranthene by Sphingobium sp. strain KK 22 and identification of new products of non-alternant PAH biodegradation by liquid chromatography electrospray ionization tandem mass spectrometry[J]. Microbial Biotechnology, 2014, 7(2): 114-129.
48	RODRIGUES E M, VIDIGAL P M P, PYLRO V S, et al. Draft genome of Nocardia farcinica TRH1, a linear and polycyclic aromatic hydrocarbon-degrading bacterium isolated from the Coast of Trindade Island, Brazil[J]. Brazilian Journal of Microbiology, 2017, 48(3): 391-392.
49	ZEINALI M, VOSSOUGHI M, ARDESTANI S K. Naphthalene metabolism in Nocardia otitidiscaviarum strain TSH1, a moderately thermophilic microorganism[J]. Chemosphere, 2008, 72(6): 905-909.
50	GU H P, YAN K, YOU Q, et al. Soil indigenous microorganisms weaken the synergy of Massilia sp. WF₁ and Phanerochaete chrysosporium in phenanthrene biodegradation[J]. Science of the Total Environment, 2021, 781: 146655.
51	TAHA M, SHAHSAVARI E, ABURTO-MEDINA A, et al. Bioremediation of biosolids with Phanerochaete chrysosporium culture filtrates enhances the degradation of polycyclic aromatic hydrocarbons (PAHs)[J]. Applied Soil Ecology, 2018, 124: 163-170.
52	DING J, CONG J, ZHOU J, et al. Polycyclic aromatic hydrocarbon biodegradation and extracellular enzyme secretion in agitated and stationary cultures of Phanerochaete chrysosporium [J]. Journal of Environmental Sciences, 2008, 20(1): 88-93.
53	BOGAN B W, LAMAR R T. Polycyclic aromatic hydrocarbon-degrading capabilities of Phanerochaete laevis HHB-1625 and its extracellular ligninolytic enzymes[J]. Appl Environ Microbiol, 1996, 62(5): 1597-1603.
54	HADIBARATA T, KRISTANTI R A. Potential of a white-rot fungus Pleurotus eryngii F032 for degradation and transformation of fluorene[J]. Fungal Biology, 2014, 118(2): 222-227.
55	WIRASNITA R, HADIBARATA T. Potential of the white-rot fungus pleurotus pulmonarius F043 for degradation and transformation of fluoranthene[J]. Pedosphere, 2016, 26(1): 49-54.
56	NIKIFOROVA S V, POZDNYAKOVA N N, TURKOVSKAYA O V. Emulsifying agent production during PAHs degradation by the white rot fungus pleurotus ostreatus D1[J]. Current Microbiology, 2009, 58(6): 554-558.
57	POLCARO C M, BRANCALEONI E, DONATI E, et al. Fungal bioremediation of creosote-treated wood: a laboratory scale study on creosote components degradation by pleurotus ostreatus mycelium[J]. Bulletin of Environmental Contamination and Toxicology, 2008, 81(2): 180-184.
58	RATHANKUMAR A K, SAIKIA K, RAMACHANDRAN K, et al. Effect of soil organic matter (SOM) on the degradation of polycyclic aromatic hydrocarbons using Pleurotus dryinus IBB 903: a microcosm study[J]. Journal of Environmental Management, 2020, 260: 110153.
59	JOHANNES C, MAJCHERCZYK A, HÜTTERMANN A. Degradation of anthracene by laccase of Trametes versicolor in the presence of different mediator compounds[J]. Applied Microbiology and Biotechnology, 1996, 46(3): 313-317.
60	HAN M J, CHOI H T, SONG H G. Degradation of phenanthrene by Trametes versicolor and its laccase[J]. Journal of Microbiology, 2004, 42(2): 94-98.
61	甄静, 李冠杰, 杜志敏, 等. 菌株Trametes hirsuta zlh237发酵液对污染土壤中多环芳烃的降解及群落结构分析[J]. 南方农业学报, 2020, 51(1): 72-79.
	ZHEN Jing, LI Guanjie, DU Zhimin, et al. Biodegradation and community structure analysis of polycyclic aromatic hydrocarbon(PAH)in contaminated soil by Trametes hirsuta zlh237 fermentation liquid[J]. Journal of Southern Agriculture, 2020, 51(1): 72-79.
62	WULANDARI R, LOTRAKUL P, PUNNAPAYAK H, et al. Toxicity evaluation and biodegradation of phenanthrene by laccase from Trametes polyzona PBURU 12[J]. 3 Biotech, 2021, 11(1): 1-11.
63	KOSCHORRECK K, RICHTER S M, SWIERCZEK A, et al. Comparative characterization of four laccases from Trametes versicolor concerning phenolic C—C coupling and oxidation of PAHs[J]. Archives of Biochemistry and Biophysics, 2008, 474(1): 213-219.
64	ZHOU H, LI X L, HU B X, et al. Assembly of fungal mycelium-carbon nanotube composites and their application in pyrene removal[J]. Journal of Hazardous Materials, 2021, 415: 125743.
65	ZANG S Y, LI P J, YU X C, et al. Degradation of metabolites of benzo[a]pyrene by coupling Penicillium chrysogenum with KMnO₄ [J]. Journal of Environmental Sciences, 2007, 19(2): 238-243.
66	ARANDA E, GODOY P, REINA R, et al. Isolation of Ascomycota fungi with capability to transform PAHs: insights into the biodegradation mechanisms of Penicillium oxalicum [J]. International Biodeterioration & Biodegradation, 2017, 122: 141-150.
67	MAHAJAN M, MANEK D, VORA N, et al. Fungi with high ability to crunch multiple polycyclic aromatic hydrocarbons (PAHs) from the pelagic sediments of Gulfs of Gujarat[J]. Marine Pollution Bulletin, 2021, 167: 112293.
68	JOVÉ P, OLIVELLA M À, CAMARERO S, et al. Fungal biodegradation of anthracene-polluted cork: a comparative study[J]. Journal of Environmental Science and Health Part A: Toxic/Hazardous Substances & Environmental Engineering, 2016, 51(1): 70-77.
69	TENG C, WU S M, GONG G Y. Bio-removal of phenanthrene, 9-fluorenone and anthracene-9, 10-dione by laccase from Aspergillus Niger in waste cooking oils[J]. Food Control, 2019, 105: 219-225.
70	DELL'ANNO A, BEOLCHINI F, CORINALDESI C, et al. Assessing the efficiency and eco-sustainability of bioremediation strategies for the reclamation of highly contaminated marine sediments[J]. Marine Environmental Research, 2020, 162: 105101.
71	LISOWSKA K, PAŁECZ B, DŁUGOŃSKI J. Detoxification of phenanthrene by C. elegans evaluated by calorimetry[J]. Thermochimica Acta, 2005, 430(1/2): 43-46.
72	CUTRIGHT T J. Polycyclic aromatic hydrocarbon biodegradation and kinetics using Cunninghamella echinulata var. elegans [J]. International Biodeterioration & Biodegradation, 1995, 35(4): 397-408.
73	POTHULURI J V, FREEMAN J P, EVANS F E, et al. Fungal metabolism of acenaphthene by Cunninghamella elegans [J]. Applied and Environmental Microbiology, 1992, 58(11): 3654-3659.
74	NARRO M L, CERNIGLIA C E, BAALEN C VAN, et al. Metabolism of phenanthrene by the marine cyanobacterium Agmenellum quadruplicatum PR-6[J]. Applied and Environmental Microbiology, 1992, 58(4): 1351-1359.
75	CERNIGLIA C E, GIBSON D T, BAALEN C V. Algal oxidation of aromatic hydrocarbons: formation of 1-naphthol from naphthalene by Agmenellum quadruplicatum, strain PR-6[J]. Biochemical and Biophysical Research Communications, 1979, 88(1): 50-58.
76	ABED R M M, KÖSTER J. The direct role of aerobic heterotrophic bacteria associated with cyanobacteria in the degradation of oil compounds[J]. International Biodeterioration & Biodegradation, 2005, 55(1): 29-37.
77	GARCÍA DE LLASERA M P, LEÓN SANTIAGO M, LOERA FLORES E J, et al. Mini-bioreactors with immobilized microalgae for the removal of benzo(a)anthracene and benzo(a)pyrene from water[J]. Ecological Engineering, 2018, 121: 89-98.
78	LUO L J, XIAO Z Y, ZHOU X Y, et al. Quantum chemical calculation to elucidate the biodegradation pathway of methylphenanthrene by green microalgae[J]. Water Research, 2020, 173: 115598.
79	SAFONOVA E, KVITKO K, KUSCHK P, et al. Biodegradation of phenanthrene by the green alga scenedesmus obliquus ES-55[J]. Engineering in Life Sciences, 2005, 5(3): 234-239.
80	HERNÁNDEZ BLANCO F J, GARCÍA DE LLASERA M P. Monitoring dihydrodiol polyaromatic hydrocarbon metabolites produced by the freshwater microalgae Selenastrum capricornutum [J]. Chemosphere, 2016, 158: 80-90.
81	CHAN S M N, LUAN T G, WONG M H, et al. Removal and biodegradation of polycyclic aromatic hydrocarbons by Selenastrum capricornutum [J]. Environmental Toxicology and Chemistry, 2006, 25(7): 1772-1779.
82	LUO L J, WANG P, LIN L, et al. Removal and transformation of high molecular weight polycyclic aromatic hydrocarbons in water by live and dead microalgae[J]. Process Biochemistry, 2014, 49(10): 1723-1732.
83	沈礼来, 丁佳锋, 钟宇驰, 等. 农田重金属阻控的微生物修复技术研究进展[J]. 广东化工, 2019, 46(22): 61-63.
	SHEN Lilai, DING Jiafeng, ZHONG Yuchi, et al. Research progress on microbial remediation technology of heavy metal control in farmland[J]. Guangdong Chemical Industry, 2019, 46(22): 61-63.
84	潘云飞, 唐正, 彭欣怡, 等. 石油烃污染土壤微生物修复技术研究现状及进展[J]. 化工进展, 2021, 40(8): 4562-4572.
	PAN Yunfei, TANG Zheng, PENG Xinyi, et al. Microbial remediation techniques for petroleum hydrocarbons contaminated soil: a review[J]. Chemical Industry and Engineering Progress, 2021, 40(8): 4562-4572.
85	ZENELI A, KASTANAKI E, SIMANTIRAKI F, et al. Monitoring the biodegradation of TPH and PAHs in refinery solid waste by biostimulation and bioaugmentation[J]. Journal of Environmental Chemical Engineering, 2019, 7(3): 103054.
86	KOSHLAF E, SHAHSAVARI E, HALEYUR N, et al. Effect of biostimulation on the distribution and composition of the microbial community of a polycyclic aromatic hydrocarbon-contaminated landfill soil during bioremediation[J]. Geoderma, 2019, 338: 216-225.
87	TAYLOR L T, JONES D M. Bioremediation of coal tar PAH in soils using biodiesel[J]. Chemosphere, 2001, 44(5): 1131-1136.
88	FERRARO A, MASSINI G, MIRITANA V M, et al. Bioaugmentation strategy to enhance polycyclic aromatic hydrocarbons anaerobic biodegradation in contaminated soils[J]. Chemosphere, 2021, 275: 130091.
89	WANG B B, TENG Y, XU Y F, et al. Effect of mixed soil microbiomes on pyrene removal and the response of the soil microorganisms[J]. Science of the Total Environment, 2018, 640/641: 9-17.
90	WANG Q, WU X G, JIANG L H, et al. Effective degradation of Di-n-butyl phthalate by reusable, magnetic Fe₃O₄ nanoparticle-immobilized Pseudomonas sp. W1 and its application in simulation[J]. Chemosphere, 2020, 250: 126339.
91	钱林波, 元妙新, 陈宝梁. 固定化微生物技术修复PAHs污染土壤的研究进展[J]. 环境科学, 2012, 33(5): 1767-1776.
	QIAN Linbo, YUAN Miaoxin, CHEN Baoliang. Research progress about bioremediation of polycyclic aromatic hydrocarbons contaminated soil with immobilized microorganism technique[J]. Environmental Science, 2012, 33(5): 1767-1776.
92	WANG B, XU X Y, YAO X W, et al. Degradation of phenanthrene and fluoranthene in a slurry bioreactor using free and Ca-alginate-immobilized Sphingomonas pseudosanguinis and Pseudomonas stutzeri bacteria[J]. Journal of Environmental Management, 2019, 249: 109388.
93	田秀梅, 王晓丽, 彭士涛, 等. 乙酸改性苎麻纤维固定化微生物的石油污染修复研究[J]. 应用化工, 2019, 48(9): 2045-2049.
	TIAN Xiumei, WANG Xiaoli, PENG Shitao, et al. Petroleum pollution remediation of acetic acid modified ramie fiber carrier immobilized microorganism[J]. Applied Chemical Industry, 2019, 48(9): 2045-2049.
94	WANG X, SUN S, LU J, et al. Remediating chlorpyrifos-contaminated soil using immobilized microorganism technology[J]. Polish Journal of Environmental Studies, 2018, 28(1): 349-357.
95	QIAO K L, TIAN W J, BAI J, et al. Removal of high-molecular-weight polycyclic aromatic hydrocarbons by a microbial consortium immobilized in magnetic floating biochar gel beads[J]. Marine Pollution Bulletin, 2020, 159: 111489.
96	DUAN L C, NAIDU R, THAVAMANI P, et al. Managing long-term polycyclic aromatic hydrocarbon contaminated soils: a risk-based approach[J]. Environmental Science and Pollution Research, 2015, 22(12): 8927-8941.
97	梁雪涛, 苏俊杰, 王震, 等. 两株菲降解菌的降解特性及动力学[J]. 净水技术, 2020, 39(S2): 40-48.
	LIANG Xuetao, SU Junjie, WANG Zhen, et al. Degradation characteristics and kinetics of two phenanthrene-degrading bacteria[J]. Water Purification Technology, 2020, 39(S2): 40-48.
98	袁林杰. 石油源多环芳烃降解菌的筛选及其降解途径的初步研究[D]. 桂林: 桂林理工大学, 2020.
	YUAN Linjie. The isolation of polycyclic aromatic hydrocarbons degrading bacteria and the study on polycyclic aromatic hydrocarbons degradation pathways[D]. Guilin: Guilin University of Technology, 2020.
99	BOUCHEZ M, BLANCHET D, VANDECASTEELE J P. Degradation of polycyclic aromatic hydrocarbons by pure strains and by defined strain associations: inhibition phenomena and cometabolism[J]. Applied Microbiology and Biotechnology, 1995, 43(1): 156-164.
100	刘帅, 刘欢, 魏海峰, 等. 4种多环芳烃对虾夷扇贝(Patinopecten yessoensis)幼体的急性毒性研究[J]. 生态毒理学报, 2020, 15(5): 352-360.
	LIU Shuai, LIU Huan, WEI Haifeng, et al. Acute toxicity of four PAHs to larva of Patinopecten yessoensis [J]. Asian Journal of Ecotoxicology, 2020, 15(5): 352-360.
101	PINELLI D, FAVA F, NOCENTINI M, et al. Bioremediation of a polycyclic aromatic hydrocarbon-contaminated soil by using different aerobic batch bioreactor systems[J]. Journal of Soil Contamination, 1997, 6(3): 243-256.
102	KOTTERMAN M J J, HEESSELS E, JONG E, et al. The physiology of anthracene biodegradation by the white-rot fungus Bjerkandera sp. strain BOS55[J]. Applied Microbiology and Biotechnology, 1994, 42(1): 179-186.
103	郭光, 田芳, 丁克强, 等. 土著B[a]P降解菌群的富集及最佳降解条件研究[J]. 农业环境科学学报, 2021, 40(1): 123-128.
	GUO Guang, TIAN Fang, DING Keqiang, et al. Enrichment and degradation characteristics of an indigenous benzo[a]pyrene-degrading bacterial consortium[J]. Journal of Agro-Environment Science, 2021, 40(1): 123-128.
104	黄秀秀, 左宇环, 李腾飞, 等. 外加碳源及固定化对荧蒽降解菌降解性能的强化作用[J]. 水生态学杂志, 2020, 41(3): 107-114.
	HUANG Xiuxiu, ZUO Yuhuan, LI Tengfei, et al. Increasing the effectiveness of a fluoranthene-degrading microorganism by immobilization and adding a carbon source[J]. Journal of Hydroecology, 2020, 41(3): 107-114.
105	罗俊鹏. 多环芳烃降解菌的筛选、降解特性及其与化学氧化联合应用研究[D]. 南昌: 南昌大学, 2019.
	LUO Junpeng. Isolation, degradation characteristics of PAHs-degrading bacteria and its application in combination with chemical oxidation[D]. Nanchang: Nanchang University, 2019.
106	曾秋玲. 石油烃降解菌的筛选及降解特性研究[D]. 大连: 大连海事大学, 2020.
	ZENG Qiuling. Screening and degradation characteristics of petroleum hydrocarbon degrading bacteria[D]. Dalian: Dalian Maritime University, 2020.
107	SHEN Y, SHENG Y, LI J F, et al. The role of temperature in phenanthrene transfer and accumulation in crop leaves[J]. Environmental Pollution, 2020, 258: 113827.
108	王仁女. 一株菲降解菌CFP312筛选及其增溶生物降解研究[D]. 赣州: 江西理工大学, 2020.
	WANG Rennv. Screening of phenanthrene-degrading strain CFP312 and its solubilization and biodegradation[D]. Ganzhou: Jiangxi University of Science and Technology, 2020.
109	YAGI J M, SIMS D, BRETTIN T, et al. The genome of Polaromonas naphthalenivorans strain CJ2, isolated from coal tar-contaminated sediment, reveals physiological and metabolic versatility and evolution through extensive horizontal gene transfer[J]. Environmental Microbiology, 2009, 11(9): 2253-2270.
110	DING G C, HEUER H, ZÜHLKE S, et al. Soil type-dependent responses to phenanthrene as revealed by determining the diversity and abundance of polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase genes by using a novel PCR detection system[J]. Applied and Environmental Microbiology, 2010, 76(14): 4765-4771.
111	THOMAS F, CÉBRON A. Short-term rhizosphere effect on available carbon sources, phenanthrene degradation, and active microbiome in an aged-contaminated industrial soil[J]. Frontiers in Microbiology, 2016, 7: 92.
112	NÍ CHADHAIN S M, NORMAN R S, PESCE K V, et al. Microbial dioxygenase gene population shifts during polycyclic aromatic hydrocarbon biodegradation[J]. Applied and Environmental Microbiology, 2006, 72(6): 4078-4087.
113	CÉBRON A, NORINI M P, BEGUIRISTAIN T, et al. Real-Time PCR quantification of PAH-ring hydroxylating dioxygenase (PAH-RHDα) genes from Gram positive and Gram negative bacteria in soil and sediment samples[J]. Journal of Microbiological Methods, 2008, 73(2): 148-159.
114	CERNIGLIA C E. Biodegradation of polycyclic aromatic hydrocarbons[J]. Current Opinion in Biotechnology, 1993, 4(3): 331-338.
115	ROJO-NIETO E, PERALES-VARGAS-MACHUCA J A. Microbial degradation of PAHs: organisms and environmental compartments[M]//Microbial degradation of xenobiotics. Berlin: Springer. 2012: 263-290.

种类	属名	菌株命名	PAHs	文献来源
细菌	红球菌属（Rhodococcus）	A2-3、qingshengii FF、P14、BAP-1、IcdP1	萘、芴、菲、芘、苯并芘、荧蒽、蒽、屈、苯并蒽、苯并荧蒽	［14，16-21］
	假单胞菌属（Pseudomonas）	SA3、ISTPY2、PA06、AH-40、PB1、PB2、P2	萘、芘、菲、芴	［22-26］
	棒杆菌属（Corynebacterium）	HRJ4	萘、菲、芘	［27-29］
	微球菌属（Micrococcus）	D12	萘、菲、荧蒽、芘	［30-32］
	产碱杆菌属（Alcaligenes）	YGD2906、BDB4、DFA4、AFS-5	菲、芘、苊、屈	［33-37］
	分枝杆菌属（Mycobacterium）	WY10、SBSW、YOWG、SKEY、EPa45、NJS-1、NJS-P、A1-PYR	菲、荧蒽、芘、荧蒽、蒽、苯并芘、芴、苯并蒽	［38-42］
	鞘脂菌属（Sphingobium）	MP9-4、FB3、B1、A4、KK22	菲、蒽、荧蒽、芘、苯并芘、萘、苊烯、苊、芴、屈、苯并荧蒽、茚并芘、苯并苝	［43-47］
放线菌	诺卡氏菌属（Nocardia）	TRH1、TSH1	萘、菲、芘、蒽	［48-49］
真菌	平革菌属（Phanerochaete）	HHB1625	菲、萘、芘、苯并蒽、蒽、苝、苯并芘	［50-53］
	侧耳属（Pleurotus）	F032、F043、D1、IBB903-A	芴、荧蒽、菲、蒽、芘、屈、萘、苊、苊烯、苯并菲、苯并蒽、苯并荧蒽、苯并芘、苝	［54-58］
	云芝属（Trametes）	Zlh237、PBURU12	蒽、菲、芘、苯并芘、芴、蒽、苊、苊烯	［59-63］
	青霉属（Penicillium）	SYJ-1、NPDF1239-K3-F21	芘、苯并芘、菲、蒽、萘、荧蒽	［64-67］
	曲霉属（Aspergillus）	NPDF190-C1-26	萘、芘、菲、蒽、荧蒽、苊、芴、苯并蒽、屈、苯并荧蒽、苯并芘、苯并苝、茚并芘	［67-70］
	小克银汉霉属（Cunninghamella）	IM1785/21Gp、ATCC36112	菲、萘、苊、菲、蒽、荧蒽、芘、苯并蒽、苯并荧蒽、苯并芘、茚并芘	［71-73］
藻类	阿格门氏藻属（Agmenellum）	PR-6	菲、萘	［74-75］
	颤藻属（Oscillatoria）	JCM、OSC	萘、菲	［75-76］
	栅藻属（Scenedesmus）	ES-55	苯并蒽、苯并芘、甲基菲、菲	［77-79］
	月牙藻属（Selenastrum）		苯并芘、苯并蒽、苯并荧蒽、菲、荧蒽、芘、二苯并蒽、茚并芘、苯并苝	［80-82］

种类	属名	菌株命名	PAHs	文献来源
细菌	红球菌属（Rhodococcus）	A2-3、qingshengii FF、P14、BAP-1、IcdP1	萘、芴、菲、芘、苯并芘、荧蒽、蒽、屈、苯并蒽、苯并荧蒽	［14，16-21］
	假单胞菌属（Pseudomonas）	SA3、ISTPY2、PA06、AH-40、PB1、PB2、P2	萘、芘、菲、芴	［22-26］
	棒杆菌属（Corynebacterium）	HRJ4	萘、菲、芘	［27-29］
	微球菌属（Micrococcus）	D12	萘、菲、荧蒽、芘	［30-32］
	产碱杆菌属（Alcaligenes）	YGD2906、BDB4、DFA4、AFS-5	菲、芘、苊、屈	［33-37］
	分枝杆菌属（Mycobacterium）	WY10、SBSW、YOWG、SKEY、EPa45、NJS-1、NJS-P、A1-PYR	菲、荧蒽、芘、荧蒽、蒽、苯并芘、芴、苯并蒽	［38-42］
	鞘脂菌属（Sphingobium）	MP9-4、FB3、B1、A4、KK22	菲、蒽、荧蒽、芘、苯并芘、萘、苊烯、苊、芴、屈、苯并荧蒽、茚并芘、苯并苝	［43-47］
放线菌	诺卡氏菌属（Nocardia）	TRH1、TSH1	萘、菲、芘、蒽	［48-49］
真菌	平革菌属（Phanerochaete）	HHB1625	菲、萘、芘、苯并蒽、蒽、苝、苯并芘	［50-53］
	侧耳属（Pleurotus）	F032、F043、D1、IBB903-A	芴、荧蒽、菲、蒽、芘、屈、萘、苊、苊烯、苯并菲、苯并蒽、苯并荧蒽、苯并芘、苝	［54-58］
	云芝属（Trametes）	Zlh237、PBURU12	蒽、菲、芘、苯并芘、芴、蒽、苊、苊烯	［59-63］
	青霉属（Penicillium）	SYJ-1、NPDF1239-K3-F21	芘、苯并芘、菲、蒽、萘、荧蒽	［64-67］
	曲霉属（Aspergillus）	NPDF190-C1-26	萘、芘、菲、蒽、荧蒽、苊、芴、苯并蒽、屈、苯并荧蒽、苯并芘、苯并苝、茚并芘	［67-70］
	小克银汉霉属（Cunninghamella）	IM1785/21Gp、ATCC36112	菲、萘、苊、菲、蒽、荧蒽、芘、苯并蒽、苯并荧蒽、苯并芘、茚并芘	［71-73］
藻类	阿格门氏藻属（Agmenellum）	PR-6	菲、萘	［74-75］
	颤藻属（Oscillatoria）	JCM、OSC	萘、菲	［75-76］
	栅藻属（Scenedesmus）	ES-55	苯并蒽、苯并芘、甲基菲、菲	［77-79］
	月牙藻属（Selenastrum）		苯并芘、苯并蒽、苯并荧蒽、菲、荧蒽、芘、二苯并蒽、茚并芘、苯并苝	［80-82］

环境条件	污染物	最佳条件	重要结论	文献来源
土壤水分	总石油烃（TPH）和多环芳烃（PAHs）	湿度为15%～30%	过高的湿度限制了空气在地下的流动，从而降低了氧气的可用性，而缺乏水分则抑制了微生物的活动	［85］
土壤含氧量	PAHs	液相体系对（PAHs去除效果显著优于半固相体系）	相对均匀的液相生物反应器保证了较高的氧传质，从而导致体系中PAHs的解吸速率、好氧菌的活性均高于半固相体系	［101］
土壤含氧量	蒽	培养阶段中增加曝气	促进蒽的生物降解及其过氧化物酶介导的氧化产物蒽醌的积累	［102］
营养物质含量	苯并[a]芘	添加适量的酵母粉	提高污染物的生物可降解性	［103］
营养物质含量	荧蒽	添加适量的麦芽糖	营养物质的加入促进微生物的生长代谢	［104］
土壤酸碱度	菲	pH=7	酸碱度过高或过低导致微生物活性降低或者死亡	［105］
土壤酸碱度	总石油烃（TPH）	pH=7.5	土壤的酸碱度直接影响微生物的生物活性	［106］
温度	菲	维持适当的培养温度	植物组织的质外体和共质体中的菲积累量与温度具有相关性	［107］
温度	菲	培养温度为30～37℃	温度直接影响微生物的生长繁殖及其代谢活动	［108］

环境条件	污染物	最佳条件	重要结论	文献来源
土壤水分	总石油烃（TPH）和多环芳烃（PAHs）	湿度为15%～30%	过高的湿度限制了空气在地下的流动，从而降低了氧气的可用性，而缺乏水分则抑制了微生物的活动	［85］
土壤含氧量	PAHs	液相体系对（PAHs去除效果显著优于半固相体系）	相对均匀的液相生物反应器保证了较高的氧传质，从而导致体系中PAHs的解吸速率、好氧菌的活性均高于半固相体系	［101］
土壤含氧量	蒽	培养阶段中增加曝气	促进蒽的生物降解及其过氧化物酶介导的氧化产物蒽醌的积累	［102］
营养物质含量	苯并[a]芘	添加适量的酵母粉	提高污染物的生物可降解性	［103］
营养物质含量	荧蒽	添加适量的麦芽糖	营养物质的加入促进微生物的生长代谢	［104］
土壤酸碱度	菲	pH=7	酸碱度过高或过低导致微生物活性降低或者死亡	［105］
土壤酸碱度	总石油烃（TPH）	pH=7.5	土壤的酸碱度直接影响微生物的生物活性	［106］
温度	菲	维持适当的培养温度	植物组织的质外体和共质体中的菲积累量与温度具有相关性	［107］
温度	菲	培养温度为30～37℃	温度直接影响微生物的生长繁殖及其代谢活动	［108］

[1]	YANG Jing, LI Bo, LI Wenjun, LIU Xiaona, TANG Liuyuan, LIU Yue, QIAN Tianwei. Degradation of naphthalene by degrading bacteria isolated from coking contaminated sites [J]. Chemical Industry and Engineering Progress, 2023, 42(8): 4351-4361.
[2]	TAN Lipeng, SHEN Jun, WANG Yugao, LIU Gang, XU Qingbai. Research progress on blending modification of coal tar pitch and petroleum asphalt [J]. Chemical Industry and Engineering Progress, 2023, 42(7): 3749-3759.
[3]	LIU Baicheng, LI Fayun, ZHAO Qihui, LIN Meixia. Research progress on remediation of polycyclic aromatic hydrocarbons contaminated soil by Gramineae plants [J]. Chemical Industry and Engineering Progress, 2023, 42(7): 3736-3748.
[4]	PAN Yunfei, TANG Zheng, PENG Xinyi, GAO Pin. Microbial remediation techniques for petroleum hydrocarbons contaminated soil: a review [J]. Chemical Industry and Engineering Progress, 2021, 40(8): 4562-4572.
[5]	Daocheng LIU, Jiuzhan WANG, Jieying JING, Zhifen YANG, Jie FENG, Wenying LI. Research progress on the catalysts for saturated hydrogenation of polycyclic aromatic hydrocarbons [J]. Chemical Industry and Engineering Progress, 2021, 40(2): 835-844.
[6]	WANG Wangyang, LIU Cong, YUAN Pei. Advances on the removal of polycyclic aromatic hydrocarbons in environment by adsorption [J]. Chemical Industry and Engineering Progree, 2017, 36(01): 355-363.
[7]	YU Hongyan，XU Feng，CHEN Hongyun，YANG Weiqun，YANG Zhenzhen. Modification of humic acid and its washing effect of PAHs in soil [J]. Chemical Industry and Engineering Progree, 2013, 32(03): 697-701.
[8]	XU Junqiang，GUO Fang，QUAN Xuejun，ZHAO Qinghua，CHU Wei. Degradation of heterocyclic compounds and polycyclic aromatic hydrocarbons in coking wastewater [J]. Chemical Industry and Engineering Progree, 2008, 27(7): 973-.

Advances in microbial remediation of soils polluted by polycyclic aromatic hydrocarbons

多环芳烃污染土壤的微生物修复技术研究进展

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 115

Related Articles 8

Recommended Articles

Metrics

Comments