Research progress on stimulated emission depletion using fluorescent probes

doi:10.16085/j.issn.1000-6613.2018-1145

Abstract

Abstract:

Stimulated emission depletion super-resolution microscopy(STED) can overcome the limit of optical diffraction to achieve the ultra-fine imaging of nanoscale of targets. The fluorescent probes play great roles in this technique. In this paper, the basic concept of STED microscopy was introduced, including the basic optical concept, principle of STED microscopy and imaging system, and STED fluorescent probes and the biological applications were summarized. This paper would be beneficial to the researchers from the fields of chemistry and chemical engineering in understanding of fundamental theory of superresolution imaging microscopy, which is helpful for the design of effective fluorescent probes in STED superresolution imaging. This review also provided the new requirement for fluorescent probes in biomedical imaging area, and the new opportunity for the joint disciplines in chemistry and chemical engineering with optical imaging.

Key words: imaging resolution, superresolution imaging, stimulated emission, stimulated emission depletion superresolution imaging microscopy, superresolution imaging probes

摘要：

受激辐射损耗超分辨成像可突破光学衍射极限的限制，获得纳米尺寸结构的超精细图像，荧光探针发挥了重要作用。本文主要介绍了受激辐射损耗超分辨显微成像的相关概念，包括基础光学概念、受激辐射损耗超分辨成像原理、成像系统，总结了受激辐射损耗超分辨成像荧光探针及其应用等研究进展，相信本工作能帮助化学、化工领域相关工作者了解受激辐射超分辨成像研究及其生物成像应用，尤其是可以用于该成像技术的荧光探针的相关知识，为设计、制备有效的受激辐射超分辨荧光探针提供设计思路。本文为荧光探针在生物医学光学领域的应用提出了新的需求与机遇，为化学、化工领域相关研究方向与光学成像领域的深度交叉与融合提供了新的发展契机。

关键词: 成像分辨率, 超分辨成像, 受激辐射, 受激辐射损耗超分辨成像, 超分辨成像探针

CLC Number:

Wufan LIU, Chufang CHEN, Wenhui PAN, Jia XIONG, Junle QU, Zhigang YANG. Research progress on stimulated emission depletion using fluorescent probes[J]. Chemical Industry and Engineering Progress, 2019, 38(02): 726-739.

刘毋凡, 陈楚芳, 潘文慧, 熊佳, 屈军乐, 杨志刚. 受激辐射损耗超分辨荧光成像探针研究进展[J]. 化工进展, 2019, 38(02): 726-739.

Figures/Tables 13

References 45

1	ABBE E . Beiträge zur theori des mikroskops und der mikroskopischen wahrnehmung[J]. Archiv Für Mikroskopische Anatomie, 1873, 9: 413-418.
2	HELMHOLTZ H VON . Die theoretische grenze für die leistungsfähigkeit der mikroskope[J]. Aus Poggendorff’s Annalen Jubelband, 1874(s): 557-584.
3	HELL S W , KROUG M . Ground-state-depletion fluorescence microscopy: a concept for breaking the diffraction resolution limits[J]. Applied Physics B: Lasers Optics, 1995, 60(5): 495-497.
4	HARKE B , ELLER J , ULLAL C K , et al . Resolution scaling in STED microscopy[J]. Optics Express, 2008, 16(6): 4154-4162.
5	HELL S W , JAKOBS S , KASTRUP L . Imaging and writing at the nanoscale with focused visible light through saturable optical transitions[J]. Applied Physics A: Materials Science Process, 2003, 77(7): 859-860.
6	HELL S W . Strategy for far-field optical imaging and writing without diffraction limit[J]. Physics Letters A, 2004, 326(1/2): 140-145.
7	HEINTZMANN R , CREMER C G . Lateral modulated excitation microscopy: improvement of resolution by using a diffraction grating[J]. Proceeding of Society Photonics International Electronics, 1998, 3568:185-196.
8	FROHN J T , KNAPP H F , STEMMER A . True optical resolution beyond the Rayleigh limit achieved by standing wave illumination[J]. Proceedings of the National Academy of Sciences U.S. A ., 2000, 97 (13): 7232-7236.
9	GUSTAFSSON M G L . Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy[J]. Journal Microscopy, 2000, 198: 82-87.
10	GUSTAFSSON M G L . Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution[J]. Proceedings of the National Academy of Sciences U.S.A., 2005, 102(37): 13081-13086.
11	BETZIG E , PATTERSON G H , SOUGRAT R ,et al . Imaging intracellular fluorescent proteins at nanometer resolution[J]. Science, 2006, 313 (5793): 1642-1645.
12	RUST M J , BATES M , ZHUANG X . Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)[J]. Nature Methods, 2006, 3(10): 793-795.
13	HESS S T , GIRIRAJAN T P K , MASON M D . Ultra-high resolution imaging by fluorescence photoactivation localization microscopy[J]. Biophysical Journal, 2006, 91(11): 4258-4272.
14	DICKSON R M , CUBITT A B , TSIEN R Y , et al . On/off blinking and switching behavior of single molecules of green fluorescent protein[J]. Nature, 1997, 388(6640): 355-358.
15	PATTERSON G H , LIPPINCOTT-SCHWARTZ J . A photoactivatable GFP for selective photolabeling of proteins and cells[J]. Science, 2002, 297(5588): 1873-1877.
16	HARKE B , KELLERR J , ULLAL C K , et al . Resolution scaling in STED microscopy[J]. Optics Express, 2008, 16(6): 4154-4162.
17	HELL S W , JAKOBS S , KASTRUP L . Imaging and writing at the nanoscale with focused visible light through saturable optical transitions[J]. Applied Physics A: Material Science Process, 2003, 77(7): 859-860.
18	HELL S W . Strategy for far-field optical imaging and writing without diffraction limit[J]. Physics Letters A, 2004, 326(1/2):
	140−145.
19	DERTINGER T , COLYER R , IYER G , et al . Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI)[J]. Proceedings of National Academy Sciences U.S.A., 2009, 106(52): 22287-22292.
20	DERTINGER T , COLYER R , VOGEL R , et al . Achieving increased resolution and more pixels with superresolution optical fluctuation imaging [J]. Optics Express, 2010, 18(18): 18875-18885. (SOFI)
21	WU D , SHEN Y , CHEN J , et al . Naphthalimide-modified near-infrared cyanine dye with a large stokes shift and its application in bioimaging[J]. Chinese Chemical Letters, 2017, 28: 1979-1982.
22	XU Z , CHEN J , HU L-L , et al . Recent advances in formaldehyde-responsive fluorescent probes[J]. Chinese Chemical Letters, 2017, 28: 1935-1942.
23	HOTTA J , FRON E , DEDECKER P , et al . Spectroscopic rationale for efficient stimulated-emission depletion microscopy fluorophores[J]. Journal of American Chemical Society, 2010, 132: 5021-5023.
24	WURM C A , KOLMAKOV K , GOTTERT F , et al . Novel red fluorophores with superior performance in STED microscopy[J]. Optical Nanoscopy, 2012, 1:7.
25	SCHILL H , NIZAMOV S , BOTANELLI F , et al . 4-Trifluoromethyl-substituted coumarins with large stokes shifts: synthesis, bioconjugates, and their use in super-resolution fluorescence microscopy[J]. Chemistry: European Journal, 2013, 19: 16556–16565.
26	ERDMANN R S , TAKAKURA H , THOMPSON A D ,et al . Super-resolution imaging of the golgi in live cells with a bioorthogonal ceramide probe[J]. Angewandte Chemie International Edition, 2014, 53(38): 10242-10246.
27	LUKINAVIČIUS G , REYMOND L , ĎESTE E , et al . Fluorogenic probes for live-cell imaging of the cytoskeleton[J]. Nature Methods, 2014, 11: 731-737.
28	ĎESTE E , KAMIN D , GÖTTFERT F , et al . STED nanoscopy reveals the ubiquity of subcortical cytoskeleton periodicity in living neurons[J]. Cell Reports, 2015, 10: 1246-1251.
29	KOLMAKOV K , HEBISCH E , WOLFRAM T , et al . Far-red emitting fluorescent dyes for optical nanoscopy: fluorinated silicon-rhodamines (SiRF dyes) and phosphorylated oxazines[J]. Chemistry : European Journal, 2015, 21: 13344-13356.
30	KASPER R , HARKE B , FORTHMANN C , et al . Single-molecule STED microscopy with photostable organic fluorophores[J]. Small, 2010, 6: 1379-1384.
31	HONIGMANN A , MUELLER V , TA H, et al . Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells[J]. Nature Communications, 2014, 5: 5412-5423.
32	VICIDOMINI G , TA H, HONIGMANN A , et al . STED-FLCS: an advanced tool to reveal spatiotemporal heterogeneity of molecular membrane dynamics[J]. Nano Letters, 2015, 15(9): 5912-5918.
33	HANNE J , FALK H J , GÖRLITZ F ,et al . STED nanoscopy with fluorescent quantum dots[J]. Nature Communications, 2015, 6: 7127-7135.
34	PERSSON F , BINGEN P , STAUDT T , et al . Fluorescence nanoscopy of single DNA molecules by using stimulated emission depletion (STED) [J]. Angewandte Chemie International Edition, 2011, 50: 5581-5583.
35	LUKINAVIČIUS G , BLAUKOPF C , PERSHAGEN E , et al . SiR-Hoechst is a far-Red DNA stain for live-cell nanoscopy[J]. Nature Communications, 2015, 6: 8497-8505.
36	HOFMANN M , EGGELING C , JAKOBS S , et al . Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins[J]. Proceedings of National Academy Sciences U.S.A.,2005, 102: 17565-17569.
37	HELL S W , JAKOBS S , KASTRUP L . Imaging and writing at the nanoscale with focused visible light through saturable optical transitions[J]. Applied Physics A, 2003, 77: 859-860.
38	LUKINAVIČIUS G , BLAUKOPF C , PERSHAGEN E , et al . SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy[J]. Nature Communications, 2015, 6: 8497-8505.
39	LAVIOE-CARDINAL F , JENSEN N A , WESTPHAL V , et al . Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics[J]. ChemPhysChem, 2014, 15: 655-663.
40	BRAKEMANN T , STIEL A C , WEBER G ,et al . A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching[J]. Nature Biotechnology, 2011, 29: 942-947.
41	GROTJOHANN T , TESTA I , LEUTENEGGER M , et al . Diffraction-unlimited all-optical imaging and writing with a photochromic GFP[J]. Nature, 2011, 478: 204-208.
42	LI D , QIN W , XU B , QIAN J , et al . AIE nanoparticles with high stimulated emission depletion efficiency and photobleaching resistance for long-term super-resolution bioimaging[J]. Advanced Materials, 2017, 29: 1703643-1703451.
43	HANNE J , FALK H J , GÖRLITZ F , et al . STED nanoscopy with fluorescent quantum dots[J]. Nature Communications, 2015, 6: 7217-7222.
44	LIU Y J , LU Y Q , YANG X S , et al . Amplified stimulated emission in upconversion nanoparticles for super-resolution nanoscopy[J]. Nature, 2017, 543: 229-233.
45	YE S , YAN W , ZHAO M , et al . Low-saturation-intensity, high-photostability, and high resolution STED nanoscopy assisted by CsPbBr₃ quantum dots[J]. Advanced Materials, 2018, 30(23): 1800167.

[1]	LI Mengyuan, GUO Fan, LI Qunsheng. Simulation and optimization of the third and fourth distillation columns in the recovery section of polyvinyl alcohol production [J]. Chemical Industry and Engineering Progress, 2023, 42(S1): 113-123.
[2]	QIU Mofan, JIANG Lin, LIU Rongzheng, LIU Bing, TANG Yaping, LIU Malin. Research progress of particle-scale model in chemical reaction numerical simulation of gas-solid fluidized bed [J]. Chemical Industry and Engineering Progress, 2023, 42(10): 5047-5058.
[3]	YU Shan, DUAN Yuangang, ZHANG Yixin, TANG Chun, FU Mengyao, HUANG Jinyuan, ZHOU Ying. Research progress of catalysts for two-step hydrogen sulfide decomposition to produce hydrogen and sulfur [J]. Chemical Industry and Engineering Progress, 2023, 42(7): 3780-3790.
[4]	LU Jianmei. Recent research progress of flexible adsorption materials [J]. Chemical Industry and Engineering Progress, 2023, 42(6): 2781-2798.
[5]	TAO Mengqi, LIU Meihong, KANG Yuchi. Analysis of fluid across a single cylinder and two parallel cylinders in a micro flow channel by micro-PIV [J]. Chemical Industry and Engineering Progress, 2023, 42(6): 2836-2844.
[6]	LYU Xuedong, LUO Faliang, LIN Haitao, SONG Danqing, LIU Yi, NIU Ruixue, ZHENG Liuchun. Recent progress of synthesis technology and gas barrier research of poly(butylene succinate) [J]. Chemical Industry and Engineering Progress, 2023, 42(5): 2546-2554.
[7]	PANG Nanjiong, WANG Xiaoling, LIAO Xuepin, SHI Bi. Separation of boron isotopes by collagen fibers-immobilized black wattle tannin [J]. Chemical Industry and Engineering Progress, 2023, 42(5): 2616-2625.
[8]	TIAN Yuan, LOU Shujie, MENG Shanru, YAN Jingru, XIAO Haicheng. Recent progress of Co-based catalysts for higher alcohols synthesis form syngas [J]. Chemical Industry and Engineering Progress, 2023, 42(4): 1869-1876.
[9]	ZHANG Chenguang, FENG Shuo, XING Yuye, SHEN Boxiong, SU Lichao. Research progress of isolated Cu²⁺ in copper based zeolite NH₃-SCR catalyst for diesel vehicles [J]. Chemical Industry and Engineering Progress, 2023, 42(3): 1321-1331.
[10]	SHANG Xiaobiao, LI Guangchao, XIAO Liping, BAI Yongzhen, XIAO Renyou, LI Jiajian, ZHANG Zhihao. Wave transmission performance of zirconium aluminum silicate fiberboard under large temperature gradient [J]. Chemical Industry and Engineering Progress, 2023, 42(3): 1551-1561.
[11]	DUAN Yihang, GAO Ningbo, QUAN Cui. Effect of hydrothermal treatment on pyrolysis characteristics and kinetics of oily sludge [J]. Chemical Industry and Engineering Progress, 2023, 42(2): 603-613.
[12]	HUANG Wei, CHU Zheng, REN Lei, LI Shan. Application of carbon-based solid acid in hydrogenation of nitrobenzene to p-aminophenol [J]. Chemical Industry and Engineering Progress, 2023, 42(1): 272-281.
[13]	TENG Xinyu, ZHANG Guohua, HU Chenshu, ZHU Cheng, YU Dan, LIU Di, LIU Sha. Analysis on hydrogen energy economy and low cost of hydrogen source in typical cities of China [J]. Chemical Industry and Engineering Progress, 2022, 41(12): 6295-6301.
[14]	WAN Nianfang. Research progress of membrane electrode assembly of proton exchange membrane water electrolysis for hydrogen production [J]. Chemical Industry and Engineering Progress, 2022, 41(12): 6385-6394.
[15]	CHEN Yu, WANG Jiajia, TANG Lin. Preparation and performance of floating carbon nitride photocatalyst CNx@mEP [J]. Chemical Industry and Engineering Progress, 2022, 41(12): 6477-6488.