Rapid oxidation of Fe2+ in adsorption tail liquid of in-situ uranium leaching by Acidithiobacillus ferrivorans

doi:10.16085/j.issn.1000-6613.2017-2356

Abstract

Abstract: To solve these problems, such as the slow growth and reproduction of mesophilic bacteria, slow oxidation rate of Fe²⁺ in the low temperature adsorption tail liquid, based on the field production practice of in-situ leaching uranium by acid method in Xinjiang, Acidithiobacillus ferrivorans was used as the oxidant and the bio-ceramsite was employed as the bio-carrier. Bacteria were immobilized on the surface of bio-ceramsite in the bioreactor. The law of rapid oxidation of Fe²⁺ in the tail fluid was also studied. The results showed that the selected bio-ceramsite had these characteristics of high porosity, high water permeability, high adsorption, and high specific surface area; and the bio-ceramsite had good adsorption and fixation effect on bacteria. These characteristics were beneficial to the growth and reproduction of bacteria. Therefore, the oxidation rate of bacteria on Fe²⁺ was also enhanced. Bio-ceramsite had strong acid-fastness and stability in the adsorption tail fluid with pH 1.6-1.7. When the aeration volume and temperature, flow rate of adsorption tail were 20L/h and 17-19℃, 500L/h, respectively, the continuous oxidation rate of Fe²⁺ in bioreactor could reach 0.85 g/(L·h). Compared to H₂O₂, Acidithiobacillus ferrivorans as the oxidant can save about 72.6% of the cost, which provides a powerful basis for the industrial application of bacteria as oxidant.

Key words: Acidithiobacillus ferrivorans, bioreactor, bio-ceramic, in-situ uranium leaching, oxidant, adsorption tail liquid

摘要： 为解决中温菌在低温吸附尾液中作氧化剂时生长繁殖慢、氧化Fe²⁺速率低等问题，实验结合新疆某酸法地浸采铀现场生产实际，选取耐冷嗜酸硫杆菌作为氧化剂，以生物陶粒为挂膜载体，在生物反应器内对细菌进行固定化培养，研究了细菌快速氧化吸附尾液中Fe²⁺规律。实验结果表明，选用的生物陶粒具有高孔隙率、高透水性、高吸附性和高比表面积等特点，对细菌有较好的吸附固着效果，有利于细菌的生长繁殖，提高了细菌氧化Fe²⁺速率；生物陶粒在pH 1.6~1.7的吸附尾液中具有较强的耐酸性和稳定性；当通气量为20L/h、吸附尾液温度为17~19℃、流量为500L/h时，生物反应器连续氧化Fe²⁺速率达到0.85g/（L·h）；与H₂O₂作氧化剂相比，可以节约费用约72.6%，为实现细菌作氧化剂的工业化应用提供了有力依据。

关键词: 耐冷嗜酸硫杆菌, 生物反应器, 生物陶粒, 地浸采铀, 氧化剂, 吸附尾液

CLC Number:

TF18

WANG Qingliang, CHEN Peng, HU Eming, LI Qiang, WANG Hongqiang, YANG Yihan, LI De, XU Yiqun. Rapid oxidation of Fe²⁺ in adsorption tail liquid of in-situ uranium leaching by Acidithiobacillus ferrivorans[J]. Chemical Industry and Engineering Progress, 2018, 37(10): 3995-4005.

王清良, 陈鹏, 胡鄂明, 李乾, 王红强, 阳奕汉, 李德, 徐屹群. 耐冷嗜酸硫杆菌快速氧化地浸采铀吸附尾液中Fe²⁺[J]. 化工进展, 2018, 37(10): 3995-4005.

References

[1] 周义朋, 沈照理, 史维浚, 等. 地浸采铀工艺分类方法的探讨[J]. 有色金属(冶炼部分), 2015(1):37-41. ZHOU Yipeng, SHEN Zhaoli, SHI Weijuan, et al. Discussion on technology classification of in-situ leaching uranium mining[J]. Nonferrous Metals(Extractive Metallurgy), 2015(1):37-41.
[2] 苏学斌, 刘乃忠, 沈红伟, 等. 新疆某铀矿空气预氧化矿层地浸采铀现场实验[J]. 金属矿山, 2006(12):33-36. SU Xuebin, LIU Naizhong, SHEN Hongwei, et al. Field test on in-situ leaching uranium mining by air pre-oxidation of ore stratum in Xinjiang[J]. Metal Mine, 2006(12):33-36.
[3] 王清良, 黄爱武, 杨金辉, 等. 过氧化氢的氧化特性及其在不同介质中的分解行为研究[J]. 矿冶, 2005, 14(1):59-61. WANG Qingliang, HUANG Aiwu, YANG Jinhui, et al. Study on decomposition characteristic of H₂O₂ in different mediums[J]. Mining and Metallurgy, 2005, 14(1):59-61.
[4] 史文革, 蔡萍莉. 硝酸盐作酸法地浸采铀氧化剂的分解机理探讨[J]. 南华大学学报(自然科学版), 2010, 24(3):40-43. SHI Wenge, CAI Pingli. Nitrate for acid in-situ leaching of uranium oxidant decomposition mechanism[J]. Journal of Nanhua University (Science & Technology), 2010, 24(3):40-43.
[5] 周义朋, 吉宏斌, 孙占学, 等. 酸性含Fe³⁺溶液作用下铀的溶解迁移特征[J]. 地质学报, 2016, 90(12):3554-3562. ZHOU Yipeng, JI Hongbin, SUN Zhanxue, et al. Uranium migration kinetics in acid solution containing ferric iron[J]. Acta Geologica Sinica, 2016, 90(12):3554-3562.
[6] 杨维涨, 伍云辉, 尹桂芳, 等. 512矿床微生物浸铀实验菌液培养技术研究[C]//全国铀矿大基地建设学术研讨会论文集(下). 2012. YANG Weizhang, WU Yunhui, YIN Guifang, et al. The reseach on bacterial culture technology of microbiogical leaching uranium test at 512 deposit[C]//The Construction of the National Conference on Uranium Mining Base Set. 2012.
[7] 王清良, 胡凯光, 刘迎九, 等. 伊宁铀矿512矿床地浸中细菌代替双氧水初步实验研究[J]. 铀矿冶, 1999, 18(4):262-268. WANG Qingliang, HU Kaiguang, LIU Yingjiu, et al. The study on bacteria instead of H₂O₂ for in-situ leaching uranium at.512 deposit of yining uranium mine[J]. Uranium Mining and Metallurgy, 1999, 18(4):262-268.
[8] 王清良, 胡鄂明, 余润兰, 等. 细菌低温驯化及其浸出砂岩型铀矿实验研究[J]. 矿冶工程, 2011, 31(6):6-8. WANG Qingliang, HU Eming, YU Runlan, et al. Low-temperature bacteria domestication and leaching of sandstone-type refractory uranium ore[J]. Mining and Metallurgical Engineering, 2011, 31(6):6-8.
[9] 朱宏飞, 李辉, 刘东奇. 三种浸矿细菌协同作用的回顾及展望[J]. 微生物学通报, 2016, 43(12):2730-2737. ZHU Hongfei, LI Hui, LIU Dongqi. A review of synergy development and prospect of three leaching bacteria[J]. Microbiology China, 2016, 43(12):2730-2737.
[10] 陈川, 孙占学, 李江. 浸铀菌群挂膜连续培养工艺研究[J]. 有色金属(矿山部分), 2014, 66(3):6-8. CHEN Chuan, SUN Zhanxue, LI Jiang. Bio-film continuous culture technology of uranium-leaching bacteria[J]. Nonferrous Metals(Mine Section), 2014, 66(3):6-8.
[11] CORAHUASANTO R, ECA A, ABANTO M, et al. Physiological and comparative genomic analysis of Acidithiobacillus ferrivorans PQ33 provides psychrotolerant fitness evidence for oxidation at low temperature[J]. Research in Microbiology, 2017, 168(5):482-492.
[12] LILJEQVIST M, VALDES J, HOLMES D S, et al. Draft genome of the psychrotolerant acidophile Acidithiobacillus ferrivorans SS3[J]. Journal of Bacteriology, 2011, 193(16):4304-4305.
[13] 邱冠周, 柳建设, 王淀佐, 等. 氧化亚铁硫杆菌生长过程铁的行为[J]. 中南工业大学学报, 1998, 29(3):226-228. QIU Guanzhou, LIU Jianshe, WANG Dianzuo, et al. Iron behavior in growth of Thiobacillus ferrooxidans.[J]. Journal of Central South University, 1998, 29(3):226-228.
[14] IUPAC manual of symbols and terminology[J]. Pure Appl. Chem., 1972, 31:578-638.
[15] 近藤精一, 石川达雄, 安部郁夫. 吸附科学[M]. 李国希, 译. 北京:化学工业出版社, 2006. KONDOU S, ISHIKAWA T, ABE I. Adsorption science[M]. LI G X, trans. Beijing:Chemical Industry Press, 2006.
[16] 辛勤, 罗孟飞.现代催化研究方法[M]. 北京:科学出版社. 2009:214-244. XIN Qin, LUO Mengfei. Modern catalytic research methods[M]. Beijing:Science Press, 2009:214-244.
[17] 叶茂友, 严苹方, 孙水裕, 等. 氧化亚铁硫杆菌生物浸出铅锌硫化矿尾矿及浸出过程中重金属形态分析研究[J]. 环境科学学报, 2016, 36(11):4102-4111. YE Maoyou, YAN pingfang, SUN Shuiyu, et al. Study on bioleaching of heavy metals in lead-zinc mine tailings by Acidithiobacillus ferrooxidans and the transformation in the speciation of heavy metals during the bioleaching[J]. Acta Scientiae Circumstantiae, 2016, 36(11):4102-4111.
[18] 许晓芳, 林海, 董颖博, 等. 阳离子对嗜酸氧化亚铁硫杆菌氧化活性的影响[J]. 稀有金属, 2016, 40(5):478-484. XU Xiaofang, LIN Hai, DONG Yingbo, et al. Oxidation activity of Acidthiobacillus Ferrooxidans with cation additives[J]. Chinese Journal of Rare Metals, 2016, 40(5):478-484.
[19] 张成桂, 夏金兰, 邱冠周. 嗜酸氧化亚铁硫杆菌亚铁氧化系统研究进展[J]. 中国有色金属学报, 2006, 16(7):1239-1249. ZHANG Chenggui, XIA Jinlan, QIU Guanzhou. Progress in research on Fe²⁺ oxidation system of Acidithiobacillus ferrooxidans[J]. Transactions of Nonferrous Metals Society of China, 2006, 16(7):1239-1249.
[20] VLAMAKIS H, CHAI Y, BEAUREGARD P, et al. Sticking together:building a biofilm the Bacillus subtilis way[J]. Nature Reviews Microbiology, 2013, 11(3):157-168.
[21] BREITENBUCHER K, SIEGL M, KNUPFER A, et al. Open-pore sintered glass as a high-efficiency support medium in bioreactors:new results and long-term experiences achieved in high-rate anaerobic digestion[J]. Water Science & Technology, 1990, 22:25-32.
[22] 丁文川, 曾晓岚, 王永芳, 等.生物炭载体的表面特征和挂膜性能研究[J]. 中国环境科学, 2011, 31(9):1451-1455. DING Wenchuan, ZENG Xiaolan, WANG Yonfang, et al. Characteristics and performances of biofilm carrier prepared from agro-based biochar.[J]. China Environmental Science, 2011, 31(9):1451-1455.
[23] CHEN C Y, KAO C M, CHEN S C, et al. Application of immobilized cells to the treatment of cyanide wastewater[J]. Water Science & Technology:A Journal of the International Association on Water Pollution Research, 2007, 56(7):99.
[24] ELBEHTI A, BRASSEUR G, LEMESLEMEUNIER D. First evidence for existence of an uphill electron transfer through the bc1 and NADH-Q oxidoreductase complexes of the acidophilic obligate chemolithotrophic ferrous ion-oxidizing bacterium Thiobacillus ferrooxidans.[J]. Journal of Bacteriology, 2000, 182(12):3602-3606.
[25] 韩洁, 马喜平, 罗螣.铁离子稳定剂的实验室评价[J]. 化学工程与装备, 2010(9):18-20. HAN Jie, MA Xiping, LUO Teng. Laboratory evaluation of iron ion stabilizer[J]. Chemical Engineering & Equipment, 2010(9):18-20.

Rapid oxidation of Fe²⁺ in adsorption tail liquid of in-situ uranium leaching by Acidithiobacillus ferrivorans

耐冷嗜酸硫杆菌快速氧化地浸采铀吸附尾液中Fe²⁺

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LU Shaojie, LIU Jia, JI Qianzhu, LI Ping, HAN Yueyang, TAO Min, LIANG Wenjun. Preparation of diatomaceous earth-based composite filler and its xylene removal performance by a biotrickling filter [J]. Chemical Industry and Engineering Progress, 2023, 42(7): 3884-3892.
[2]	OUYANG Sufang, ZHOU Daowei, HUANG Wei, JIA Feng. Research progress on novel anti-migration rubber antioxidants [J]. Chemical Industry and Engineering Progress, 2023, 42(7): 3708-3719.
[3]	ZHANG Han, ZHANG Xiaojing, MA Bingbing, NAI Can, LIU Shuoshuo, MA Yongpeng, SONG Yali. Feasibility of starting anammox process with municipal waste sludge as seed sludge [J]. Chemical Industry and Engineering Progress, 2023, 42(2): 1080-1088.
[4]	LIU Yajuan. Research status of membrane fouling mitigation by PAC in submerged PAC-AMBRs [J]. Chemical Industry and Engineering Progress, 2023, 42(1): 457-468.
[5]	XU Yabing, WANG Baoshan, WANG Guangzong, ZHANG Yang. Degradation of refractory organics in the pharmaceutical wastewater by bioelectrochemical system [J]. Chemical Industry and Engineering Progress, 2022, 41(9): 5055-5064.
[6]	WANG Na, SONG Xiulan, ZAN Botao. Synthesis of PHA by mixed microorganisms using simulative hydrolysate liquid from the excess sludge by APG combined with FNA pretreatment [J]. Chemical Industry and Engineering Progress, 2022, 41(2): 1017-1024.
[7]	GAO Hao, LU Jiasheng, ZHANG Wenming, DONG Weiliang, FANG Yan, YU Ziyi, XIN Fengxue, JIANG Min. Application of material-mediated cell immobilization technology in biological fermentation [J]. Chemical Industry and Engineering Progress, 2021, 40(7): 3923-3931.
[8]	DU Jiahui, LIU Jia, YANG Juping, QI Hongyi, DOU Xiaona, LI Jian. Recent advances in biological combined technology for VOCs treatment [J]. Chemical Industry and Engineering Progress, 2021, 40(5): 2802-2812.
[9]	MA Yueyuan, CHEN Jinchun, CHEN Guoqiang. Halophilic microorganisms as microbial chassis: applications and prospects [J]. Chemical Industry and Engineering Progress, 2021, 40(3): 1178-1186.
[10]	ZHANG Lanhe, YUAN Zhentao, ZHAO Haojie, ZHAO Juntian, ZHU Yining, CHEN Zicheng, JIA Yanping, TIAN Shulei. Effect of external electric current on denitrification efficiency and sludge flocculation of anoxic zone using AO process [J]. Chemical Industry and Engineering Progress, 2021, 40(11): 6369-6377.
[11]	Hao JIANG, Fan WU, Lei YU, Mingyu QIAN, Hongjun ZHOU, Yeqing LI. Characteristics of anaerobic fermentation in CSTR, VPF and BVPF for yellow storage of corn straw [J]. Chemical Industry and Engineering Progress, 2020, 39(8): 3256-3262.
[12]	Hailiang XING,Xunzan DONG,Benyong HAN,Shuxiang GENG,Delu NING,Ting MA,Xuya YU. Cell growth and lipid accumulation of Monoraphidium sp. QLZ-3 in walnut shell extracts with carbon dioxide [J]. Chemical Industry and Engineering Progress, 2020, 39(4): 1575-1582.
[13]	Hui LI, Ganlu LI, Feng HE, Xu XU, Weilong TIAN, Sheng XU, Kequan CHEN, Pingkai OUYANG. Research progress of microbubble coupled bioreactor [J]. Chemical Industry and Engineering Progress, 2020, 39(12): 4758-4765.
[14]	Wen TENG, Yong CHEN, Meng SUI, Fashe LI. Effect of TEPA and [MI][C₆H₂(OH)₃COO] compound on antioxidant property of biodiesel [J]. Chemical Industry and Engineering Progress, 2020, 39(11): 4427-4434.
[15]	Yunzhi QIAN,Huaji MA,Hongying YUAN,Yanmei DING,Yifan ZHANG,Sufeng TIAN,Yuyou LI,Yongzhi CHI,Bowen ZHANG. Partial nitrification start-up and operation process of internal circulation contact oxidation membrane bioreactor [J]. Chemical Industry and Engineering Progress, 2019, 38(9): 3995-4002.